Some properties of cytochrome b5 from liver microsomes of man, monkey, pig and chicken.
Biochem J
; 115(4): 849-56, 1969 Dec.
Article
em En
| MEDLINE
| ID: mdl-4982419
ABSTRACT
1. Cytochrome b(5) was released from liver microsomes of man, monkey, pig and chicken by incubation with a crude lipase preparation. 2. By using DEAE-cellulose chromatography, ammonium sulphate fractionation, Sephadex-gel filtration and a final gradient elution on DEAE-Sephadex A-50, cytochromes b(5) were obtained from the four species studied, all possessing similar spectral properties. 3. Stokes radii of the cytochromes were measured by gel filtration. 4. N-Terminal amino acids for the different cytochromes were serine for man and monkey, alanine for pig and glycine for chicken. 5. Amino acid analyses of the cytochromes are presented. 6. Peptide ;fingerprint' patterns of tryptic digests of the different cytochromes are discussed and clearly show increasing similarity for more closely related species.
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Microssomos Hepáticos
/
Citocromos
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Biochem J
Ano de publicação:
1969
Tipo de documento:
Article