Alpha-actinin accumulation in epithelial cells infected with attaching and effacing gastrointestinal pathogens.
J Infect Dis
; 172(5): 1393-6, 1995 Nov.
Article
em En
| MEDLINE
| ID: mdl-7594686
ABSTRACT
Fluorescent F-actin staining utilizing phalloidin, a highly toxic mushroom poison, is used as an indirect test to detect attaching and effacing (AE) bacteria. A study was done to determine if accumulation of alpha-actinin in infected tissue culture cells is a consistent feature and whether it corresponds with the AE response. Rearrangement of alpha-actinin was detected using immunofluorescence microscopy by incubation of infected cells with a murine monoclonal anti-alpha-actinin antibody. Foci of alpha-actinin-specific fluorescence corresponding to areas of bacterial adhesion were detected by transmission electron microscopy in HEp-2 and gastric KATO-III cells infected with only those bacterial strains that formed AE lesions. Therefore, this study shows that alpha-actinin accumulation is a consistent, specific manifestation of the AE phenotype and forms the basis for the development of a safe alternative test for detecting AE bacteria.
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Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Aderência Bacteriana
/
Actinina
/
Helicobacter pylori
/
Helicobacter
/
Escherichia coli
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Infect Dis
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Canadá