Destabilization of rbcS sense transcripts by antisense RNA.
Plant Mol Biol
; 25(3): 569-76, 1994 Jun.
Article
em En
| MEDLINE
| ID: mdl-8049381
ABSTRACT
Steady-state rbcS mRNA levels are drastically reduced in transgenic tobacco plants that express rbcS antisense RNAs. We have found that these reductions are not due to an effect of the antisense RNA at the level of rbcS transcription; rather, the sense mRNAs are more actively degraded in the mutant than wild-type plants. We have examined the kinetics of this turnover process by inhibiting transcription with cordycepin, and have found that rbcS sense mRNA decay is accelerated about five-fold in the antisense plants. This provides direct evidence that antisense RNAs can serve to destabilize sense transcripts in plants.
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Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Ribulose-Bifosfato Carboxilase
/
RNA Mensageiro
/
RNA Antissenso
Idioma:
En
Revista:
Plant Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
/
BOTANICA
Ano de publicação:
1994
Tipo de documento:
Article