Clustered organization and conservation of the Xiphophorus maculatus D locus, which includes two distinct gene sequences.
Chromosoma
; 105(4): 242-9, 1996 Oct.
Article
em En
| MEDLINE
| ID: mdl-8854884
ABSTRACT
Genomic organization and chromosomal localization of a previously uncharacterized D (Donor) locus in Xiphophorus and Poecilia species was investigated using fluorescence in situ hybridization (FISH) and Southern blot analysis. Part of this region is thought to be involved in the recombination event leading to formation of the Xmrk oncogene and it has recently been shown that this locus includes two different genes, one with high homology to a zinc finger protein of the Krüppel type, and the other an unknown gene with high similarity to a Caenorhabditis elegans gene. FISH to Xiphophorus chromosomes revealed that these two unrelated genes are closely linked and clustered at a unique chromosomal site. Southern blot hybridization patterns suggest that these genes exist in the genome as multiple copies. Furthermore, similar genomic organization profiles seem to prevail among other related fish. In particular, our FISH experiments reveal the existence of a conserved homologous chromosomal segment harboring the zinc finger protein sequence in several poeciliid fish.
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Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Ciprinodontiformes
/
Família Multigênica
/
Dedos de Zinco
Limite:
Animals
Idioma:
En
Revista:
Chromosoma
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Alemanha