Establishment of breast cell cultures and lines from peoples of African origin.
Am J Obstet Gynecol
; 176(6): S240-5, 1997 Jun.
Article
em En
| MEDLINE
| ID: mdl-9215215
ABSTRACT
OBJECTIVES:
The purpose of our study was to establish breast epithelial cell cultures and cell lines from peoples of African origin (blacks). It is presumed that the biology of breast cancer in women of African origin has unique aspects that can be explored using cultured breast epithelial cells. STUDYDESIGN:
Biopsy specimens were obtained from black women undergoing radical or modified mastectomies. Normal cell cultures were established using tissue from reduction mammaplasties or the milk of lactating mothers. The tissue specimens were lacerated, digested with collagenase solution, and plated on tissue culture plates. To extend the life of the epithelial cells in culture, they are transformed with SV40 virus.RESULTS:
We have maintained breast tumor cells in culture from a 27-year-old black woman for more than 1 month.CONCLUSION:
Despite the difficulty of establishing epithelial cell cultures, we have maintained breast tumor cells from blacks in culture for an extended period to allow characterization.
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Coleções:
01-internacional
Temas:
Geral
/
Tipos_de_cancer
/
Outros_tipos
Base de dados:
MEDLINE
Assunto principal:
Mama
/
Neoplasias da Mama
/
Técnicas de Cultura de Células
/
População Negra
Limite:
Adult
/
Female
/
Humans
País/Região como assunto:
Africa
Idioma:
En
Revista:
Am J Obstet Gynecol
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Estados Unidos