Chronic ethanol exposure alters leukocyte subsets in repopulating spleens, but does not alter negative selection in thymuses of sublethally irradiated mice.
Alcohol Clin Exp Res
; 21(8): 1520-9, 1997 Nov.
Article
em En
| MEDLINE
| ID: mdl-9394126
ABSTRACT
Results from previous in vitro experiments in this laboratory suggested that ethanol may affect selection processes in the thymus. To determine whether ethanol allows escape of potentially autoreactive T-cell clones from negative selection, we fed ethanol to sublethally irradiated, young, adult C57BR mice during the time of thymic and splenic repopulation as a new model of human third trimester fetal alcohol exposure. The mice received a whole-body, sublethal dose (6 Gy) of gamma irradiation at 5 to 6 weeks of age. Feeding of a liquid diet providing 25% of calories as ethanol (EDC) or an isocaloric control liquid diet was begun 3 days after irradiation and was continued for 5 weeks. Each EDC mouse had 2 weight- and age-matched controls, 1 pair-fed (PF), and 1 fed ad libitum (AD LIB). Average blood alcohol concentrations (90 to 440 mg/100 ml) were higher than those reported previously for neonatal mice exposed to ethanol through lactation. At 5 weeks after irradiation, the EDC mice had lower total thymocyte numbers (p < 0.05) and a higher proportion of CD4-CD8-thymocytes than either the PF or AD LIB mice (p < 0.05), which is consistent with findings using in utero models of ethanol exposure. Ethanol exposure also altered the proportion of leukocyte subsets in repopulating spleens. B cells were the most sensitive to the detrimental effects of ethanol and, as a percentage of total nucleated cells in the spleen, B cells were decreased in the EDC group, compared with both the PF and AD LIB groups (p < 0.05). C57BR mice normally delete by negative selection thymocytes bearing v beta 17 T-cell receptors. There was no discernible effect of ethanol exposure during thymic and splenic repopulation on the expression of V beta 17a on thymocytes and splenic T lymphocytes, indicating that ethanol does not affect negative selection.
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Coleções:
01-internacional
Temas:
Geral
/
Prevencao_e_fatores_de_risco
/
Alcoolismo
/
Tratamento
Base de dados:
MEDLINE
Assunto principal:
Baço
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Timo
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Subpopulações de Linfócitos B
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Subpopulações de Linfócitos T
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Leucopoese
/
Etanol
/
Alcoolismo
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Female
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Humans
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Newborn
/
Pregnancy
Idioma:
En
Revista:
Alcohol Clin Exp Res
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Estados Unidos