A novel spliced transcript of human CLAPS2 encoding a protein alternative to clathrin adaptor protein AP17.
Gene
; 220(1-2): 39-44, 1998 Oct 05.
Article
em En
| MEDLINE
| ID: mdl-9767099
ABSTRACT
Transcripts of genes encoding proteins of clathrin complexes have been reported to undergo tissue-specific alternative splicing. AP17, encoded by human CLAPS2 cDNA, is the small chain of the major clathrin adaptor complex AP-2 associated with mammalian plasma membranes. In this study, two cDNAs were isolated from a cDNA library of human blood cells. Whereas one cDNA encoded AP17, the other cDNA encoded a putative novel protein variant, termed AP17Delta. Both coding regions were completely sequenced. Consisting of 142aa residues, the predicted protein AP17Delta of 12kDa lacks 38aa residues of AP17. Using specific primers for RT-PCR, mRNAs for AP17Delta and AP17 were found in leukocytes and cultured leukemia cells. The finding of a putative intron in a human EST cDNA clone suggests that mRNAs for AP17 and AP17Delta are formed by alternative splicing. In addition, the identity of human and rat AP17 amino acid sequences is demonstrated.
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Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Fosfoproteínas
/
Clatrina
/
Processamento Alternativo
/
Complexo 2 de Proteínas Adaptadoras
/
Subunidades sigma do Complexo de Proteínas Adaptadoras
/
Proteínas do Tecido Nervoso
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Gene
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Áustria