Safety of Special Waveform of Transcranial Electrical Stimulation (TES): In Vivo Assessment.

Intermittent theta burst (iTBS) powered by direct current stimulation (DCS) can safely be applied transcranially to induce neuroplasticity in the human and animal brain cortex. tDCS-iTBS is a special waveform that is used by very few studies, and its safety needs to be confirmed. Therefore, we aimed to evaluate the safety of tDCS-iTBS in an animal model after brain stimulations for 1 h and 4 weeks. Thirty-one Sprague Dawley rats were divided into two groups: (1) short-term stimulation for 1 h/session (sham, low, and high) and (2) long-term for 30 min, 3 sessions/week for 4 weeks (sham and high). The anodal stimulation applied over the primary motor cortex ranged from 2.5 to 4.5 mA/cm2. The brain biomarkers and scalp tissues were assessed using ELISA and histological analysis (H&E staining) after stimulations. The caspase-3 activity, cortical myelin basic protein (MBP) expression, and cortical interleukin (IL-6) levels increased slightly in both groups compared to sham. The serum MBP, cortical neuron-specific enolase (NSE), and serum IL-6 slightly changed from sham after stimulations. There was no obvious edema or cell necrosis seen in cortical histology after the intervention. The short- and long-term stimulations did not induce significant adverse effects on brain and scalp tissues upon assessing biomarkers and conducting histological analysis.

Environmental risk assessment of the emerging EDCs contaminants from rural soil and aqueous sources: Analytical and modelling approaches.

The emerging endocrine disrupting chemicals posed high risk and much uncertainty to eco-environment and human health. An analytical method, developed for the simultaneous determination of five steroid estrogens in groundwater and soil based upon solid phase extraction and gas chromatography-mass spectrometry, was applied to investigate the distribution of estrone and 17ß-estradiol around Shenyang City with particular focus on penetrating from surface to groundwater in this study. Mean concentrations of the estrone and 17ß-estradiol were 55.1 ng L-1 and 56.1 ng L-1 in groundwater, 32.5 ng g-1 and 23.1 ng g-1 in soil, respectively. The distribution of estrone and 17ß-estradiol were similar in groundwater, the concentration in the west of the site center was relatively low, and the surroundings were relatively high. The concentration of estrone was changed less, but 17ß-estradiol was significantly increased in silt and silty sand layers in vadose zone profiles. Both estrone and 17ß-estradiol concentrations changed abruptly at the interface of layers. Incorporating the temporal and spatial evolution of physical-chemical-biological environmental parameters at the sites, sorption and biodegradation were suggested the controlling roles in the fate and transport of SEs in the soil-groundwater system. The Ecological risk quotients values of both soil and groundwater indicated a very high ecological risk associated with SEs, but the non-carcinogenic harm quotients did not exceed the acceptable level of non-carcinogenic human health risk.