An assessment of sampling methods and microbiological hygiene indicators for process verification in poultry slaughterhouses.

Studies to determine the appropriateness of the use of populations of indicator bacteria on poultry carcasses for process verification were undertaken in commercial slaughterhouses. Samples were collected from neck skin by excision or from whole carcass rinses and were examined for a range of presumptive process hygiene indicator bacteria. Coefficients of variation were calculated for each bacterial indicator and were significantly lower in excised samples, indicating more reproducible bacterial recovery by this sampling method. Total viable counts of aerobic bacteria, Enterobacteriaceae, and Pseudomonas in samples collected by excision had the lowest coefficients of variation when compared with other indicators and were therefore used for further study. The uncertainties associated with the quantification of each bacterial indicator were calculated and were lowest overall for total viable counts of aerobic bacteria. In general, uncertainty was higher for lower bacterial numbers. Results of microbiological testing on pooled excised neck skin samples were not significantly different from the mean of individually analyzed samples. Bacterial numbers increased by 1 log unit when cultures were stored under chilled conditions typical of those used for transporting samples to external laboratories, but the increases were not significant for Pseudomonas and aerobic bacteria when storage time was less than 17 h. Weak relationships were identified between bacterial indicator numbers and duration of processing, although cleanliness of the processing environment diminished visibly during this time. In the plants visited for this study, there was a poor relationship between presumptive bacterial indicator numbers and process hygiene. Consequently, bacterial analyses for process verification purposes may be of limited value.

Assessment and development of procedures and apparatus to reduce contamination of lamb carcasses during pelt removal in low-throughput abattoirs.

Two series of experiments were carried out to investigate methods of reducing contamination of lamb carcasses in low-throughput abattoirs, where cradle dressing is normally employed. In the first series, cradle design and pelt removal procedure were investigated, and a method was developed for assessing gross visible contamination. Significant improvements in microbiological and gross visible contamination (P < 0.01) were achieved by procedural changes only; modifications to the cradle design had no effect. In the second series of experiments, two improved methods of pelt removal and the effect of hand washing prior to carcass contact during the pelt removal procedure were investigated. The improved methods comprised a Frame system, in which the pelt was removed in a manner similar to that in a high-throughput inverted line, and a Hybrid system, in which the pelt was removed from the forequarters on a conventional cradle before the carcass was suspended in an "inverted" vertical position for removal of the pelt from the abdomen and hindquarters. The results of microbiological and gross visible contamination from these methods, with and without hand washing, were compared with the conventional Cradle method of pelt removal. Both the Hybrid and Frame systems had significantly less microbiological and gross visible contamination (P < 0.01). However, hand washing had no significant effect on the level of carcass contamination for all three methods of pelt removal. Greatest reductions in microbiological and gross visible contamination were achieved using techniques that minimized hand contact with the carcass during pelt removal by adoption of inverted dressing procedures. Equipment redesign did not reduce carcass contamination.

Validation of an automated technique for determining the mechanical characteristics of coronary arteries during balloon angioplasty: laboratory assessment with necropsy segments.

OBJECTIVES: To develop a technique for automatic inflation of a percutaneous transluminal coronary angioplasty (PTCA) balloon, with continuous measurement of the balloon pressure and volume; to validate the technique for determining the mechanical characteristics of coronary arteries. METHODS: During necropsy examination of the hearts of nine patients, 17 coronary artery samples were obtained for histological examination. A PTCA balloon was inserted into each artery, and the balloon pressure and volume were measured continuously during four repeat automatic inflations of the balloon. RESULTS: Of the 17 arteries, eight showed elastic, six plastic, and three fracture pressure-volume deformation characteristics. For the plastic deformations, the first inflation required a higher pressure than subsequent repeat inflations of 82 (61) kPa (mean (SD), range 25 to 175 kPa). For the three in the fracture group, the pressure drop because of the fracture occurred between 210 and 540 kPa. Two of these three showed a tear on visual inspection, and the other showed disruption of the intimal plaque on blinded histological examination. Of the six with plastic deformation characteristics alone, one showed a tear, and on histological examination two others showed splitting of the internal and external elastic lamina and one showed separation of intima and media. None in the elastic group showed any of these characteristics. CONCLUSIONS: Plastic and fracture deformation characteristics could be differentiated from elastic characteristics. Visual or histological evidence of fracturing was present in all three arteries identified during angioplasty as having pressure-volume fracture characteristics.

Influence of grazing cattle and sheep together and separately on animal performance and forage quality.

Cattle and sheep grazed together and separately from April to October during 3 yr. Initial forage composition was 29% Kentucky bluegrass (Poa pratensis L.), 11% white clover (Trifolium repens L.), and 60% weeds. There were six Angus cow-calf pairs or six ewes (1/2 Dorset x 1/4 Finn x 1/4 Rambouillet) with 11 lambs per each of three pasture replications for single animal species. Six cow-calf pairs plus six ewes and 11 lambs grazed in each of three replications of the mixed animal species treatment. There were approximately one cow and calf or five ewes with lambs per .44 ha. Lambs were weaned at 41 kg or by September 1. Calves were weaned approximately October 10. Lamb daily gain (.23 kg/d), total gain (23 kg), and weaning weights (43 kg) were greater (P < .01) and target weaning weights were reached 14 d earlier in the grazing season when both animal species grazed together than when lambs were in pastures with sheep alone (.18 kg/d, 19 and 38 kg, respectively). Calf gains were not influenced by treatment. Grazing pressure increased throughout the grazing season for cattle alone, peaked in midsummer, and then decreased by autumn for sheep alone, whereas grazing pressure initially increased then remained relatively constant with mixed-species grazing. Forage quality in pastures where sheep grazed alone was lower (P < .05) than in pastures where cattle grazed alone until lambs were weaned in late summer, whereas forage quality with mixed-species grazing was generally intermediate. Mixed grazing resulted in earlier weaning and increased lamb performance and BW of ewes, but not in increased animal production per hectare.

A model for assessing health needs of the rural elderly: methodology and results.

The purpose of this health needs assessment of the rural elderly was to determine the actual conditions, opportunities, activities, and attitudes of older citizens living in a rural area and to assess their specific psychological, social, and medical needs. This was accomplished through a survey developed by an interdisciplinary team and distributed to individuals aged 55 years or older in a rural community of Georgia. Survey responses were used to plan and implement a series of four workshops to improve health education in the rural community surveyed. In addition, referrals to health care and social agencies were initiated where indicated.