The potential of Euglena species as a bioindicator for soil ecotoxicity assessment.

Currently, there are no standard international test methods for assessing aquatic and soil toxicity, with aquatic toxicity tests based on limited Euglena species. Here, we proposed Euglena species as extended test species, especially as new soil test species for a paper-disc soil method, considering its ecologically important roles in providing highly bioavailable in-vivo nutrients to upper trophic level organisms. We conducted experiments to identify the optimal exposure duration for two Euglena species (Euglena viridis and Euglena geniculata). We demonstrated the toxic effects of nickel (model contaminant) on their photosynthetic parameters and growth in freshwater. The growth and photosynthetic activity of three Euglena species were significantly inhibited in nickel-contaminated soil during paper-disc soil tests, especially the test species adsorbed onto paper-disc soil. Euglena gracilis was more sensitive to nickel than E. viridis and E. geniculata in freshwater and soil. Thus, E. viridis and E. geniculata have potential as additional test species for improving test species diversity, while all three species have potential as new soil test species for soil toxicity assessment. Thus, results these species may be suitable for routine aquatic toxicity testing and new soil toxicity testing, addressing the current paucity of test species in freshwater and soil toxicity assessment.

Toxicity assessment of tire particles released from personal mobilities (bicycles, cars, and electric scooters) on soil organisms.

Tire particles are generated by the abrasion of tire treads on roads and are major contributors to microplastics in soil environments. Contamination by tire wear particles worsens annually as the use of personal mobilities increases. Tire particles (112-541 µm) were obtained from three types of personal mobility tires (bicycle, car, and electric scooter) and exposed to plants (Vigna radiata) and springtails (Folsomia candida) for 28 d to assess the toxicity of each tire-particle type. The laboratory-generated tire particles exhibit adverse effects depending on the origin of the tire or test species. Particles from bicycle or electric-scooter tires changed the soil's bulk density and water holding capacity and adversely affected plant growth. Car tire particles had leached various organic compounds and induced detrimental effects on springtails (adult and offspring growth). We concluded that laboratory-generated tire particles (frow new tires) can affect the soil environment by changing soil properties and leaching chemicals; thus, causing adverse effects on soil organisms. Since this study found tire particle toxicity on soil organisms, it would be possible to compare the various contamination levels in areas near road soil and other clean soils.

Site-specific ecological risk assessment of metal-contaminated soils based on the TRIAD approach.

Ecological risk assessment based on scientific data is crucial for understanding causal relationships between chemical pollution and environmental risks. Simultaneously, a balance is required between socioeconomic factors and scientific evidence. The TRIAD approach, which incorporates three lines of evidence (LoE)-chemical (Chem-LoE), ecotoxicological (Ecotox-LoE), and ecological (Eco-LoE)-was applied in five sites of an abandoned mine for site-specific soil ecological risk assessment (SERA). In combination, the three LoEs showed that two sites had extremely high risks, one site had moderate risk, and the other site had low risk. At all sites, Chem-LoE exhibited high-integrated risk values. In Ecotox-LoE and Eco-LoE, some species were not affected despite high metal concentrations in the soil samples collected from the sites, indicating that the bioavailability of metals differed according to the physiochemical properties of the soil medium. This study is significant as multiple analyses were performed considering ecosystem structure to reduce uncertainty in SERA. The results provide information to support effective decision-making risk management to protect the soil ecosystem. Moreover, these findings will be useful in establishing policies and priorities for soil risk management.

Toward Sustainable Environmental Quality: Priority Research Questions for Asia.

Environmental and human health challenges are pronounced in Asia, an exceptionally diverse and complex region where influences of global megatrends are extensive and numerous stresses to environmental quality exist. Identifying priorities necessary to engage grand challenges can be facilitated through horizon scanning exercises, and to this end we identified and examined 23 priority research questions needed to advance toward more sustainable environmental quality in Asia, as part of the Global Horizon Scanning Project. Advances in environmental toxicology, environmental chemistry, biological monitoring, and risk-assessment methodologies are necessary to address the adverse impacts of environmental stressors on ecosystem services and biodiversity, with Asia being home to numerous biodiversity hotspots. Intersections of the food-energy-water nexus are profound in Asia; innovative and aggressive technologies are necessary to provide clean water, ensure food safety, and stimulate energy efficiency, while improving ecological integrity and addressing legacy and emerging threats to public health and the environment, particularly with increased aquaculture production. Asia is the largest chemical-producing continent globally. Accordingly, sustainable and green chemistry and engineering present decided opportunities to stimulate innovation and realize a number of the United Nations Sustainable Development Goals. Engaging the priority research questions identified herein will require transdisciplinary coordination through existing and nontraditional partnerships within and among countries and sectors. Answering these questions will not be easy but is necessary to achieve more sustainable environmental quality in Asia. Environ Toxicol Chem 2020;39:1485-1505. © 2020 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

Ecological risk assessment for perfluorooctanoic acid in soil using a species sensitivity approach.

Perfluorooctanoic acid (PFOA) is one of the persistent organic pollutants that has been listed in Annex A of the Stockholm Convention and has attracted attention owing to its endocrine-disrupting properties. However, there is currently little information available regarding the soil ecotoxicity of PFOA and the associated ecological risks. Accordingly, in this study, we sought to assess the soil ecological risk of PFOA based on a probabilistic approach using data obtained from multispecies bioassays and soil toxicity assessments, from which we generated soil species sensitivity distributions and estimated soil protective concentrations for PFOA. Using the latter distributions, we also undertook a probabilistic ecological risk assessment. On the basis of acute and chronic toxicity estimates obtained from bioassays involving eight soil-associated organisms from six diverse taxonomic groups, we could deduce that PFOA poses a negligible risk to soil ecosystems. However, we also found that this chemical may be more toxic than some of the established endocrine-disrupting chemicals such as bisphenol A, nonylphenol, and methylparaben, thereby indicating that further in-depth studies would be necessary to obtain a better understanding of the toxic potential of this chemical in the soil environment.

Application of a soil quality assessment system using ecotoxicological indicators to evaluate contaminated and remediated soils.

The deterioration of soil quality owing to human activities results in adverse effects on the soil ecosystem. This study developed a systematic method to quantitatively evaluate soil quality based on physical, chemical, biological, and ecotoxicological indicators and proposed the soil quality assessment and management system. This system consists of step-by-step processes, including indicator classification, indicator measurement, scoring and weighting, and soil quality index (SQI) calculation. The novel strategy included the usage of authentic ecotoxicological indicators for realistically interpreting soil quality assessment results. This study used five ecotoxicological indicators, including earthworm survival, enzyme activities, nematode reproduction, plant germination and growth, soil algal biomass, and soil algal photosynthetic capacity. Relatively higher SQI values than those corresponding to the actual soil quality status would be obtained without considering the ecotoxicological indicators. We conclude that the use of ecotoxicological indicator can help in soil quality assessment even under extreme soil quality conditions, such as highly contaminated or physically and chemically remediated soils.

Quantitative assessment of photosynthetic activity of Chlorella (Class Trebouxiophyceae) adsorbed onto soil by using fluorescence imaging.

In the present study, we evaluate our previously developed non-destructive soil algal toxicity method using species from a different class of algae; Class Trebouxiophyceae (Chlorella vulgaris and Chlorella sorokiniana), and directly measure the photosynthetic activity of these species adsorbed onto the soil as a new toxicity endpoint. This study shows that non-destructive soil algal toxicity method is applicable to non-specific test species, including those of Class Trebouxiophyceae as well as Class Chlorophyceae (Chlorococcum infusionum and Chlamydomonas reinhardtii). Furthermore, by performing photosynthesis image analysis, we verify that it is possible to measure the photosynthetic activity of soil algae Chlorella vulgaris adsorbed onto soils without the need to extract algal cells from the soil. We propose that the non-destructive soil algal toxicity method represents a novel technique for 1) evaluating pollutants in soil using non-specific algae and 2) conveniently and rapidly assessing the photosynthetic activity of soil algae Chlorella vulgaris adsorbed onto soil as a new toxicity endpoint.

Ecological hazard assessment of methyl ethyl ketone using the species sensitivity distribution approach in a soil ecosystem.

Methyl ethyl ketone (MEK) is a common and widely used industrial solvent. However, few studies have investigated its toxicity, or its effects as a contaminant in soil ecosystems. In this study, acute and chronic toxicity data for MEK were generated, and ecological risk based on a species sensitivity distribution was assessed. Seven soil organisms from six taxonomic groups were used for acute toxicity tests and five soil organisms from four taxonomic groups were used for chronic toxicity tests. Acute and chronic soil HC5 (hazardous concentration for 5% of species) values for MEK were estimated as 53.04 and 2.593 mg MEK/kg dry soil, respectively. This is the first study to conduct battery testing for MEK; it specifies hazardous concentrations, warns of the need for accident preparedness, and points to serious potential hazards of MEK at various levels of the soil ecosystem which can translate into greater environmental damage with implications for human health. The specific sensitivity levels determined may serve as a benchmark for establishing soil standards and strategies for ecosystem protection in the face of accidental contamination.

Development of a nematode offspring counting assay for rapid and simple soil toxicity assessment.

Since the introduction of standardized nematode toxicity assays by the American Society for Testing and Materials (ASTM) and International Organization for Standardization (ISO), many studies have reported their use. Given that the currently used standardized nematode toxicity assays have certain limitations, in this study, we examined the use of a novel nematode offspring counting assay for evaluating soil ecotoxicity based on a previous soil-agar isolation method used to recover live adult nematodes. In this new assay, adult Caenorhabditis elegans were exposed to soil using a standardized toxicity assay procedure, and the resulting offspring in test soils attracted by a microbial food source in agar plates were counted. This method differs from previously used assays in terms of its endpoint, namely, the number of nematode offspring. The applicability of the bioassay was demonstrated using metal-spiked soils, which revealed metal concentration-dependent responses, and with 36 field soil samples characterized by different physicochemical properties and containing various metals. Principal component analysis revealed that texture fraction (clay, sand, and silt) and electrical conductivity values were the main factors influencing the nematode offspring counting assay, and these findings warrant further investigation. The nematode offspring counting assay is a rapid and simple process that can provide multi-directional toxicity assessment when used in conjunction with other standard methods.

Estimation of daily fish intake values for use with water quality criteria for human health assessments in Korea.

Daily fish intake is a key parameter of water quality criteria for protecting human health. Daily fish intake values should be representative of consumption patterns and must be practical for regulatory purposes. Thus, values must be scientifically verified and regularly updated for inclusion in water quality criteria. In Korea, four different fish intake values have been identified from food balance sheets (KREI 2000), the Korea National Health and Nutrition Examination Survey (KNHANES) II (Ministry of Health and Welfare 2002), Korean Exposure Factors Handbook (MOE 2007a), and KNHANES IV-V (CDC 2008; 2009; 2010), which have been applied to water quality standards and related national projects and regulations. This paper reviews the estimation methodologies of previous daily fish intake values from multiple sources and improvements in these values between 2000 and 2012. Finally, limitations associated with each value were examined to assist future research and regulatory management. This review provides information on changes in the daily fish intake values and their application in water quality standards in Korea.

Rapid in situ assessment for predicting soil quality using an algae-soaked disc seeding assay.

The soil quality of remediated land is altered and this land consequently exerts unexpected biological effects on terrestrial organisms. Therefore, field evaluation of such land should be conducted using biological indicators. Algae are a promising new biological indicator since they are a food source for organisms in higher soil trophic levels and easily sampled from the soil. Field evaluation of soil characteristics is preferred to be testing in laboratory conditions because many biological effects cannot be duplicated during laboratory evaluations. Herein, we describe a convenient and rapid algae-soaked disc seeding assay for assessing soil quality in the field based on soil algae. The collection of algae is easy and rapid and the method predicts the short-term quality of contaminated, remediated, and amended farm and paddy soils. The algae-soaked disc seeding assay is yet to be extensively evaluated, and the method cannot be applied to loamy sand soil in in situ evaluations. The algae-soaked disc seeding assay is recommended for prediction of soil quality in in situ evaluations because it reflects all variations in the environment. The algae-soaked disc seeding assay will help to develop management strategies for in situ evaluation.

Derivation of Ecological Protective Concentration using the Probabilistic Ecological Risk Assessment applicable for Korean Water Environment: (I) Cadmium.

Probabilistic ecological risk assessment (PERA) for deriving ecological protective concentration (EPC) was previously suggested in USA, Australia, New Zealand, Canada, and Netherland. This study suggested the EPC of cadmium (Cd) based on the PERA to be suitable to Korean aquatic ecosystem. First, we collected reliable ecotoxicity data from reliable data without restriction and reliable data with restrictions. Next, we sorted the ecotoxicity data based on the site-specific locations, exposure duration, and water hardness. To correct toxicity by the water hardness, EU's hardness corrected algorithm was used with slope factor 0.89 and a benchmark of water hardness 100. EPC was calculated according to statistical extrapolation method (SEM), statistical extrapolation methodAcute to chronic ratio (SEMACR), and assessment factor method (AFM). As a result, aquatic toxicity data of Cd were collected from 43 acute toxicity data (4 Actinopterygill, 29 Branchiopoda, 1 Polychaeta, 2 Bryozoa, 6 Chlorophyceae, 1 Chanophyceae) and 40 chronic toxicity data (2 Actinopterygill, 23 Branchiopoda, 9 Chlorophyceae, 6 Macrophytes). Because toxicity data of Cd belongs to 4 classes in taxonomical classification, acute and chronic EPC (11.07 µg/l and 0.034 µg/l, respectively) was calculated according to SEM technique. These values were included in the range of international EPCs. This study would be useful to establish the ecological standard for the protection of aquatic ecosystem in Korea.

Assessment of toxic heavy metals in urban lake sediments as related to urban stressor and bioavailability.

A suite of heavy metals was monitored at sediments and overlying water in three urban lakes located in Seoul, Korea during spring season 2006. Metals measured were zinc, arsenic, chromium, copper, nickel, and cadmium. All metal concentrations in urban lake sediments were much higher than those in natural lake sediments. Elevated metal levels in urban lake sediments are associated with urban runoff, including street dust polluted by heavy metals. Metals in sediments from urban lakes were extracted with a weak electrolyte solution (0.1 M Ca(NO(3))(2)) to predict the toxicity of metals. Among the six heavy metals studied, Cu was the most extractable, followed by Ni and Zn. Ca(NO(3))(2)-extractable metal recoveries has a good relationship with metal toxicity based on Chironomus riparius bioassay. This study showed that urban stressors such as vehicle emissions could increase the concentration of heavy metals in urban lake sediments. In addition, there is a positive relationship between sediment toxicity by using C. riparius bioassay and Ca(NO(3))(2)-extractability of heavy metals from sediments.

Fridericia peregrinabunda (Enchytraeidae) as a new test species for soil toxicity assessment.

The present study proposes Fridericia peregrinabunda (Michaelsen, 1913) as a new test species for assessment of soil ecotoxicity. Fridericia is the richest genus in the Enchytraeidae family (Oligochaeta: Annelida: Clitellata: Enchytraeidae), and widely distributed. The acute toxicity of cadmium (Cd) and antimony (Sb) on the survival of F. peregrinabunda were investigated in laboratory experiments. Adult survival of F. peregrinabunda in metal spiked soil was reduced. Cadmium was more toxic to F. peregrinabunda than Sb. In the artificial soil toxicity tests, the LC50 values for F. peregrinabunda exposed to Cd and Sb for 48 h were 37, and 446 mg kg(-1), respectively. The NOEC values for Cd and Sb were 20 and 100 mg kg(-1) up to 48 h exposure, respectively. This is the first report on the ecotoxicological assay of contaminated soils using the enchytraeids F. peregrinabunda as a test species. It can be concluded that F. peregrinabunda is a suitable test species to measure the acute toxicity of heavy metals, and this species is more sensitive to Cd and Sb than earthworm. The filter paper contact test and 2 day-artificial soil toxicity test appear to be rapid and cost-effective protocols for the potworm assay.

Assessment of comparative toxicities of lead and copper using plant assay.

The acute toxicities of lead (Pb) and copper (Cu) to important crop plants Sorghum bicolor, Cucumis sativus, Triticum aestivum, and Zea mays were compared. The EC50 values (the concentration of metals in the soil that reduces the growth of shoots and roots by 50%) were derived using the Trimmed Spearman-Karber method. The EC50s-shoot (root) in mg Pb kg-1 dry soil and mg Cu kg-1 dry soil were in the range of 519 to >1280 (285-445), and 48-232 (<40-110), respectively. Those concentrations are likely to occur in some abandoned mine areas in Korea. The figures indicate that Cu is more toxic than Pb to the plants in this study, and that root growth is more sensitive to the toxicity endpoint than shoot growth in Cu- or Pb-amended soils. On the other hand, seed germination is insensitive to both Pb and Cu toxicities. The Pb- and Cu-sensitive plants were also identified. Among the plants tested, T. aestivum and S. bicolor were most sensitive to Pb and Cu, respectively. Z. mays was most resistant to both Pb and Cu. The combined effects of Pb and Cu depend on the plant species, and no general phenomenon was observed. Bioaccumulations of Pb and Cu were observed in all test species, and they are concentration-dependent. These differences in the toxicities of Pb and Cu in plant species should be taken into account in biomonitoring and ecological risk assessment.

Soil ecotoxicity assessment using cadmium sensitive plants.

Four crop plant species (sweet corn, Zea may; wheat, Triticum aestivum; cucumber, Cucumis sativus; and sorghum, Sorghum bicolor) were tested to assess an ecotoxicity in cadmium-amended soils. The measurement endpoints used were seed germination and seedling growth (shoot and root). The presence of cadmium decreased the seedling growth. The medium effective concentration values (EC50) for shoot or root growth were calculated by the Trimmed Spearman-Karber method. Due to the greater accumulation of Cd to the roots, root growth was a more sensitive endpoint than shoot growth. Bioavailability and transport of Cd within plant were related to concentration and species. The ratio of bioaccumulation factor (BAF) in the shoots to the roots indicated high immobilization of Cd in the roots. Seed germination was insensitive to Cd toxicity, and is not recommended for a suitable assay. Among the test plants and test endpoints, root growth of sorghum and cucumber appears to be a good protocol to assess ecotoxicity of soils contaminated by Cd.