The Immunology Quality Assessment Proficiency Testing Program for CD3⁺4⁺ and CD3⁺8⁺ lymphocyte subsets: a ten year review via longitudinal mixed effects modeling.

Since 1999, the National Institute of Allergy and Infectious Diseases Division of AIDS (NIAID DAIDS) has funded the Immunology Quality Assessment (IQA) Program with the goal of assessing proficiency in basic lymphocyte subset immunophenotyping for each North American laboratory supporting the NIAID DAIDS HIV clinical trial networks. Further, the purpose of this program is to facilitate an increase in the consistency of interlaboratory T-cell subset measurement (CD3(+)4(+)/CD3(+)8(+) percentages and absolute counts) and likewise, a decrease in intralaboratory variability. IQA T-cell subset measurement proficiency testing was performed over a ten-year period (January 2003-July 2012), and the results were analyzed via longitudinal analysis using mixed effects models. The goal of this analysis was to describe how a typical laboratory (a statistical modeling construct) participating in the IQA Program performed over time. Specifically, these models were utilized to examine trends in interlaboratory agreement, as well as successful passing of proficiency testing. Intralaboratory variability (i.e., precision) was determined by the repeated measures variance, while fixed and random effects were taken into account for changes in interlaboratory agreement (i.e., accuracy) over time. A flow cytometer (single-platform technology, SPT) or a flow cytometer/hematology analyzer (dual-platform technology, DPT) was also examined as a factor for accuracy and precision. The principal finding of this analysis was a significant (p<0.001) increase in accuracy of T-cell subset measurements over time, regardless of technology type (SPT or DPT). Greater precision was found in SPT measurements of all T-cell subset measurements (p<0.001), as well as greater accuracy of SPT on CD3(+)4(+)% and CD3(+)8(+)% assessments (p<0.05 and p<0.001, respectively). However, the interlaboratory random effects variance in DPT results indicates that for some cases DPT can have increased accuracy compared to SPT. Overall, these findings demonstrate that proficiency in and among IQA laboratories have, in general, improved over time and that platform type differences in performance do exist.

Assessment of magnification of digital pelvic radiographs in total hip arthroplasty using templating software.

INTRODUCTION: Templating of pelvic radiographs traditionally involved using implant-company provided acetates which assumed a magnification of 115-120%. With the introduction of digital imaging, many departments are becoming filmless. Templating software has been designed to allow on-screen templating of digital images. Knowledge of the true magnification of the image is required for accurate measurement. PATIENTS AND METHODS: Fifty consecutive postoperative pelvic radiographs were analysed using templating software. The implanted component was measured using an assumed magnification factor of 115%. The template image was then reset to the known component size, and the magnification factor was adjusted until the template covered the true component. RESULTS: An assumed magnification factor of 115% oversized the acetabular component by a mean of 6 mm (three component sizes) in all 50 components. The mean true magnification in our department was 127%. CONCLUSIONS: Validation of the true magnification produced by a radiology department using templating software is a simple and reproducible technique. It is recommended to all departments using digital images and templating software. Assumption of a magnification factor of 115% risks oversizing components by 6 mm.