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2.
Risk Anal ; 37(3): 408-420, 2017 03.
Artigo em Inglês | MEDLINE | ID: mdl-27088886

RESUMO

In this article, the performance objectives (POs) for Bacillus cereus group (BC) in celery, cheese, and spelt added as ingredients in a ready-to-eat mixed spelt salad, packaged under modified atmosphere, were calculated using a Bayesian approach. In order to derive the POs, BC detection and enumeration were performed in nine lots of naturally contaminated ingredients and final product. Moreover, the impact of specific production steps on the BC contamination was quantified. Finally, a sampling plan to verify the ingredient lots' compliance with each PO value at a 95% confidence level (CL) was defined. To calculate the POs, detection results as well as results above the limit of detection but below the limit of quantification (i.e., censored data) were analyzed. The most probable distribution of the censored data was determined and two-dimensional (2D) Monte Carlo simulations were performed. The PO values were calculated to meet a food safety objective of 4 log10 cfu of BC for g of spelt salad at the time of consumption. When BC grows during storage between 0.90 and 1.90 log10 cfu/g, the POs for BC in celery, cheese, and spelt ranged between 1.21 log10 cfu/g for celery and 2.45 log10 cfu/g for spelt. This article represents the first attempt to manage the concept of PO and 2D Monte Carlo simulation in the flow chart of a complex food matrix, including raw and cooked ingredients.


Assuntos
Bacillus cereus , Contagem de Colônia Microbiana , Manipulação de Alimentos/métodos , Gestão de Riscos/métodos , Verduras/microbiologia , Apium/microbiologia , Teorema de Bayes , Queijo/microbiologia , Contaminação de Alimentos/análise , Microbiologia de Alimentos , Inocuidade dos Alimentos , Listeria monocytogenes , Modelos Estatísticos , Método de Monte Carlo , Temperatura , Triticum/microbiologia
3.
Biomed Res Int ; 2017: 6353510, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29457031

RESUMO

A prototype decision support IT-tool for the food industry was developed in the STARTEC project. Typical processes and decision steps were mapped using real life production scenarios of participating food companies manufacturing complex ready-to-eat foods. Companies looked for a more integrated approach when making food safety decisions that would align with existing HACCP systems. The tool was designed with shelf life assessments and data on safety, quality, and costs, using a pasta salad meal as a case product. The process flow chart was used as starting point, with simulation options at each process step. Key parameters like pH, water activity, costs of ingredients and salaries, and default models for calculations of Listeria monocytogenes, quality scores, and vitamin C, were placed in an interactive database. Customization of the models and settings was possible on the user-interface. The simulation module outputs were provided as detailed curves or categorized as "good"; "sufficient"; or "corrective action needed" based on threshold limit values set by the user. Possible corrective actions were suggested by the system. The tool was tested and approved by end-users based on selected ready-to-eat food products. Compared to other decision support tools, the STARTEC-tool is product-specific and multidisciplinary and includes interpretation and targeted recommendations for end-users.


Assuntos
Contaminação de Alimentos , Inocuidade dos Alimentos , Armazenamento de Alimentos/normas , Indústria de Processamento de Alimentos , Ácido Ascórbico/química , Contagem de Colônia Microbiana , Manipulação de Alimentos , Microbiologia de Alimentos/normas , Humanos , Listeria monocytogenes/isolamento & purificação , Listeria monocytogenes/patogenicidade , Listeriose/microbiologia , Listeriose/prevenção & controle
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