A Cell-Based Assessment of the Muscle Anabolic Potential of Blue Whiting (Micromesistius poutassou) Protein Hydrolysates.

Blue whiting (BW) represents an underutilised fish species containing a high-quality protein and amino acid (AA) profile with numerous potentially bioactive peptide sequences, making BW an economic and sustainable alternative source of protein. This study investigated the impact of three different BW protein hydrolysates (BWPH-X, Y and Z) on growth, proliferation and muscle protein synthesis (MPS) in skeletal muscle (C2C12) myotubes. BWPHs were hydrolysed using different enzymatic and heat exposures and underwent simulated gastrointestinal digestion (SGID), each resulting in a high degree of hydrolysis (33.41-37.29%) and high quantities of low molecular mass peptides (86.17-97.12% <1 kDa). C2C12 myotubes were treated with 1 mg protein equivalent/mL of SGID-BWPHs for 4 h. Muscle growth and myotube thickness were analysed using an xCelligence™ platform. Anabolic signalling (phosphorylation of mTOR, rpS6 and 4E-BP1) and MPS measured by puromycin incorporation were assessed using immunoblotting. BWPH-X significantly increased muscle growth (p < 0.01) and myotube thickness (p < 0.0001) compared to the negative control (amino acid and serum free media). Muscle protein synthesis (MPS), as measured by puromycin incorporation, was significantly higher after incubation with BWPH-X compared with the negative control, but did not significantly change in response to BWPH-Y and Z treatments. Taken together, these preliminary findings demonstrate the anabolic potential of some but not all BWPHs on muscle enhancement, thus providing justification for human dietary intervention studies to confirm and translate the results of such investigations to dietary recommendations and practices.

Muscular contraction frequency does not affect plasma homocysteine concentration in response to energy expenditure- and intensity-matched acute exercise in sedentary males.

Acute exercise seems to increase total plasma homocysteine (tHcy); since this variable associated with cardiovascular risk, it is important to understand the determinants of its response to all types of exercise. The aim of this study was to examine the impact of cycling at 2 different rates of muscle contraction on the complete tHcy kinetics. Eight young sedentary males were required to complete 2 isocaloric (400 kcal) acute exercise trials at 50% peak oxygen uptake on separate occasions at 50 or 80 rpm. Blood samples were drawn at different points before (4 h before exercise and immediately before exercise), during (10, 20, 30, 45, and 60 min during exercise), and after exercise (immediately and 19 h after exercise). Dietary and lifestyle factors were controlled during the research. Maximum tHcy occurred during exercise for both conditions (50 rpm: 11.4 ± 2.7 µmol·L-1; 80 rpm: 10.8 ± 3.2 µmol·L-1). From this point onwards tHcy declined until the cessation of exercise and continued descending below pre-exercise values at 19 h postexercise (p < 0.05). No hyperhomocysteinemia were observed at any sampling point in both trials. In conclusion, the different muscular contraction frequency during exercise has no impact on tHcy during an acute bout of exercise in sedentary individuals, when at least 400 kcal are spent during exercise and the nutritional status for folate, B12, and B6 is adequate. This information is relevant to further inform healthy exercise prescription, not only in terms of duration and intensity of exercise, but also taking into account frequency of contraction.

The Effect of Strength Training on Performance Indicators in Distance Runners.

Beattie, K, Carson, BP, Lyons, M, Rossiter, A, and Kenny, IC. The effect of strength training on performance indicators in distance runners. J Strength Cond Res 31(1): 9-23, 2017-Running economy (RE) and velocity at maximal oxygen uptake (VV[Combining Dot Above]O2max) are considered to be the best physiological performance indicators in elite distance runners. In addition to cardiovascular function, RE and VV[Combining Dot Above]O2max are partly dictated by neuromuscular factors. One technique to improve neuromuscular function in athletes is through strength training. The aim of this study was to investigate the effect of a 40-week strength training intervention on strength (maximal and reactive strength), VV[Combining Dot Above]O2max, economy, and body composition (body mass, fat, and lean mass) in competitive distance runners. Twenty competitive distance runners were divided into an intervention group (n = 11; 29.5 ± 10.0 years; 72.8 ± 6.6 kg; 1.83 ± 0.08 m) and a control group (n = 9; 27.4 ± 7.2 years; 70.2 ± 6.4 kg; 1.77 ± 0.04 m). During week 0, 20, and 40, each subject completed 3 assessments: physiology (V2 mmol·L BLa, V2 mmol·L BLa [blood lactate], V4 mmol·L BLa, RE, VV[Combining Dot Above]O2max, V[Combining Dot Above]O2max), strength (1 repetition maximum back squat; countermovement jump and 0.3 m drop jump), and body composition (body mass, fat mass, overall lean, and leg lean). The intervention group showed significant improvements in maximal and reactive strength qualities, RE, and VV[Combining Dot Above]O2max, at weeks 20 (p ≤ 0.05) and 40 (p ≤ 0.05). The control group showed no significant changes at either time point. There were no significant changes in body composition variables between or within groups. This study demonstrates that 40 weeks of strength training can significantly improve maximal and reactive strength qualities, RE, and VV[Combining Dot Above]O2max, without concomitant hypertrophy, in competitive distance runners.

The Effect of Maximal- and Explosive-Strength Training on Performance Indicators in Cyclists.

Cycling economy (CE), power output at maximal oxygen uptake (WV̇O2max), and anaerobic function (ie, sprinting ability) are considered the best physiological performance indicators in elite road cyclists. In addition to cardiovascular function, these physiological indicators are partly dictated by neuromuscular factors. One technique to improve neuromuscular function in athletes is through strength training. The aim of this study was to investigate the effect of a 20-wk maximal- and explosive-strength-training intervention on strength (maximal strength, explosive strength, and bike-specific explosive strength), WV̇O2max, CE, and body composition (body mass, fat and lean mass) in cyclists. Fifteen competitive road cyclists were divided into an intervention group (endurance training and strength training: n = 6; age, 38.0 ± 10.2 y; weight, 69.1 ± 3.6 kg; height, 1.77 ± 0.04 m) and a control group (endurance training only: n = 9; age, 34.8 ± 8.5 y; weight, 72.5 ± 7.2 kg; height, 1.78 ± 0.05 m). The intervention group strength-trained for 20 wk. Each participant completed 3 assessments: physiology (CE, WV̇O2max, power at 2 and 4 mmol/L blood lactate), strength (isometric midthigh pull, squat-jump height, and 6-s bike-sprint peak power), and body composition (body mass, fat mass, overall leanness, and leg leanness). The results showed significant between- and within-group changes in the intervention group for maximal strength, bike-specific explosive strength, absolute WV̇O2max, body mass, overall leanness, and leg leanness at wk 20 (P < .05). The control group showed no significant within-group changes in measures of strength, physiology, or body composition. This study demonstrates that 20 wk of strength training can significantly improve maximal strength, bike-specific explosive strength, and absolute WV̇O2max in competitive road cyclists.

The accuracy of the SenseWear Pro3 and the activPAL3 Micro devices for measurement of energy expenditure.

Activity monitors such as the SenseWear Pro3 (SWP3) and the activPAL3 Micro (aP3M) are regularly used by researchers and practitioners to provide estimates of the metabolic cost (METs) of activities in free-living settings. The purpose of this study is to examine the accuracy of the MET predictions from the SWP3 and the aP3M compared to the criterion standard MET values from indirect calorimetry. Fifty-six participants (mean age: 39.9 (±11.5), 25M/31F) performed eight activities (four daily living, three ambulatory and one cycling), while simultaneously wearing a SWP3, aP3M and the Cosmed K4B2 (K4B2) mobile metabolic unit. Paired samples T-tests were used to examine differences between device predicted METs and criterion METs. Bland-Altman plots were constructed to examine the mean bias and limits of agreement for predicted METs compared to criterion METs. SWP3 predicted MET values were significantly different from the K4B2 for each activity (p ⩽ 0.004), excluding sweeping (p = 0.122). aP3M predicted MET values were significantly different (p < 0.001) from the K4B2 for each activity. When examining the activities collectively, both devices underestimated activity intensity (0.20 METs (SWP3), 0.95 METs (aP3M)). The greatest mean bias for the SWP3 was for cycling (-3.25 METs), with jogging (-5.16 METs) producing the greatest mean bias for the aP3M. All of the activities (excluding SWP3 sweeping) were significantly different from the criterion measure. Although the SWP3 predicted METs are more accurate than their aP3M equivalent, the predicted MET values from both devices are significantly different from the criterion measure for the majority of activities.