The metabolism and dissipation behavior of tolfenpyrad in tea: A comprehensive risk assessment from field to cup.

The metabolites of pesticides usually require rational risk assessment. In the present study, the metabolites of tolfenpyrad (TFP) in tea plants were identified using UPLC-QToF/MS analysis, and the transfer of TFP and its metabolites from tea bushes to consumption was studied for a comprehensive risk assessment. Four metabolites, PT-CA, PT-OH, OH-T-CA, and CA-T-CA, were identified, and PT-CA and PT-OH were detected along with dissipation of the parent TFP under field conditions. During processing, 3.11-50.00 % of TFP was further eliminated. Both PT-CA and PT-OH presented a downward trend (7.97-57.89 %) during green tea processing but an upward trend (34.48-124.17 %) during black tea manufacturing. The leaching rate (LR) of PT-CA (63.04-101.03 %) from dry tea to infusion was much higher than that of TFP (3.06-6.14 %). As PT-OH was no longer detected in tea infusions after 1 d of TFP application, TFP and PT-CA were taken into account in the comprehensive risk assessment. The risk quotient (RQ) assessment indicated a negligible health risk, but PT-CA posed a greater potential risk than TFP to tea consumers. Therefore, this study provides guidance for rational TFP application and suggests the sum of TFP and PT-CA residues as the maximum residual limit (MRL) in tea.

Residue behavior and risk assessment of afidopyropen and its metabolite M440I007 in tea.

Afidopyropen, a novel insecticide, is highly effective against piercing insects such as the tea leafhopper. The residual levels of afidopyropen and M440I007 in tea cultivation, processing, and brewing were studied. During tea cultivation, afidopyropen dissipated faster in fresh tea shoots in the rainy season (T1/2 of 1.2-2.5 d) than that in the dry season (T1/2 of 3.1-4.4 d); afidopyropen was metabolized into M440I007, the level of which peaked in 1 d, and degraded rapidly (over 90 %) afterward 3 d. The green tea processing steps had little effect on decreasing the afidopyropen residue (PF of 0.90-1.18). Low infusion rates of afidopyropen (16.7 %-17.7 %) and M440I007 (4.1 %-6.2 %) were observed from dry green tea to infusion; furthermore, the risk of ingesting afidopyropen from drinking tea was low, with the risk quotient values < 0.0001. This study can offer guidance on the rational application of afidopyropen in tea plants.

Dissipation behavior and risk assessment of tolfenpyrad from tea bushes to consuming.

The dissipation behavior of tolfenpyrad, a widely used pyrazole insecticide in tea plantations, was investigated during tea bushes growing, manufacturing and brewing. Tolfenpyrad dissipated fast on the tea bushes with the half-lives of 1.8-2.3 days. Manufacturing processes of green tea and black tea further reduced the tolfenpyrad residue by 3.5%-36.4%. The average processing factors (PFs) of tolfenpyrad ranged from 0.68 to 1.40 and 0.84 to 1.30 during the processing of green tea and black tea, respectively. Then a low infusion factor of 9.8%-19.9% was observed during the brewing of made tea, as the water solubility of tolfenpyrad was only 0.087 mg/L. Therefore, more than 96% of the initial deposition of tolfenpyrad was dissipated and not accessible for consuming. Results of the risk quotient (RQ) assessment also indicated a negligible health risk by tea consumption. Results from this study indicated that the residue of tolfenpyrad can be reduced by proper field management, manufacturing and brewing processes, where field dissipation and brewing were key steps to minimize its risks. Data of this study could also provide guidance for rational application of tolfenpyrad in tea plantations.

Residue analysis and dietary exposure risk assessment of acibenzolar-S-methyl and its metabolite acibenzolar acid in potato, garlic, cabbage, grape and tomato.

Acibenzolar-S-methyl (ASM) is one of the most effective plant resistance activators and protects against a broad spectrum of fungal, bacterial and viral pathogens. A rapid, efficient and high-throughput analysis method for ASM and its metabolite acibenzolar acid in fruits and vegetables was developed using potato, garlic, cabbage, grape and tomato as representative commodities by modified QuEChERS and UPLC-MS/MS. The modified procedure showed satisfying recoveries (70-108%) fortified in the range of 0.01-1 mg/kg with relative standard deviations (RSDs) lower than 17.7%. With the established analytical method, the dietary risk of ASM in fruits and vegetables from Chinese markets were further monitored using risk quotient (RQ) method. The RQ value based on ASM residue in China are far less than 1, elucidating that the potential health risk induced by ASM ingestion for Chinese population is not significant. Comparing the residue and risk assessment results of ASM in agricultural products in China to those in Codex, the maximum residue limits (MRLs) for ASM on garlic, cabbage and tomato established by CAC (Codex Alimentarius Commission) can be safely adopted in China, whereas the MRLs on potato and grape in China should be proposed as 0.01 mg/kg.

The degradation and metabolism of chlorfluazuron and flonicamid in tea: A risk assessment from tea garden to cup.

Degradation and metabolism of chlorfluazuron and flonicamid from tea garden to cup were simultaneously investigated by a modified QuEChERS method coupled with UPLC-MS/MS quantification. The dissipation half-lives of chlorfluazuron, flonicamid, and total flonicamid (the sum of flonicamid and its metabolites TFNG, TFNA, and TFNA-AM) in fresh tea leaves during tea growth were 6.0 d, 4.8 d, and 8.1 d, respectively. TFNG and TFNA were generated during tea growth. After tea processing, the residues of chlorfluazuron, flonicamid, and its metabolites in black tea were higher than those in green tea. The average processing factors of chlorfluazuron, flonicamid, and total flonicamid in black tea were 2.54, 3.02, and 2.87, respectively, while in green tea they were 2.40, 2.93, and 2.79, respectively. TFNG, TFNA, and TFNA-AM were formed rapidly during the drying step. Considering the influence of water content at various processing steps, the average loss rates of chlorfluazuron, flonicamid, and total flonicamid residue from fresh tea leaves to black tea were 16.7%, 33.8%, and 20.7%, respectively, and 29.6%, 14.0% and 18.2%, respectively, in the case of green tea. The highest leaching rates of chlorfluazuron, flonicamid, and total flonicamid during tea brewing were 6.8%, 97.0%, and 97.4%, respectively, in black tea infusion, and 6.0%, 98.9%, and 98.6%, respectively, in green tea infusion. The metabolites, especially TFNG, had a higher leaching rate during tea brewing. The migration of chlorfluazuron from fresh leaves to tea infusion was low, and the migration of flonicamid was high. The RQc and RQa of chlorfluazuron and total flonicamid were less than 1. This result indicates that the potential dietary intake risk of chlorfluazuron from tea is negligible. However, the risk of total flonicamid intake is three times higher than that of chlorfluazuron. There is a potential risk of intake of flonicamid and its metabolites in tea for human consumption.

Residue dissipation and dietary exposure risk assessment of methoxyfenozide in cauliflower and tea via modified QuEChERS using UPLC/MS/MS.

BACKGROUND: Methoxyfenozide possesses efficacy against a variety of lepidopteron pests, including the major pests in cauliflower and tea, so it is of great importance to generalize the practical use of methoxyfenozide in the field. RESULTS: An efficient method was developed and validated in both vegetable matrix and extract-rich matrix (cauliflower and tea) using modified QuEChERS combined with UPLC/MS/MS analysis. The recoveries in cauliflower, made tea and tea shoots ranged from 94.5 to 108.0%, from 85.0 to 91.6% and from 77.3 to 82.0% respectively, with relative standard deviations (RSDs) below 17.3% in all cases. The field results showed that methoxyfenozide dissipated in cauliflower with half-life (t1/2 ) at 2.5-3.5 days and in tea with t1/2 at 1.2 days. Combining the above experimental data and statistical food intake values, the risk quotient (RQ) values were significantly lower than 1. CONCLUSION: The quantification method of methoxyfenozide in cauliflower or tea has not been established until this study. The dissipation and dietary exposure risk assessment of methoxyfenozide in cauliflower and tea were investigated in the field. Methoxyfenozide dissipated rapidly in cauliflower despite different climates, and it dissipated faster in tea. The dietary risk of methoxyfenozide through cauliflower or tea was negligible to humans. This study not only provides guidance for the safe use of methoxyfenozide but also serves as a reference for the establishment of maximum residue limits (MRLs) in China. © 2019 Society of Chemical Industry.

Dissipation and Risk Assessment of Multiresidual Fungicides in Grapes under Field Conditions.

Grapes are among the most popular fruits globally, and various fungicides are widely applied to grape crops. As such, the presence of multiple fungicide residues and dietary risks in grapes has become the focus of significant attention. In this study, an easy-to-implement and sensitive UPLC-MS/MS approach was developed to simultaneously determine pyraclostrobin, dimethomorph, cymoxanil, cyazofamid and its metabolite CCIM in grapes via QuEChERS. This approach achieved 78.1-106.0% recovery and a 0.01 mg kg-1 limit of quantitation (LOQ). Field trials revealed that these compounds had degradation half-lives ranging from 0.9 to 13.3 days. And their terminal residues ranging from < LOQ to 1.36 mg kg-1 were below the official maximum residue limit (MRL) in China. The short-term risk for each tested fungicide was below 54%. The long-term risk of individual chemicals ranged from 0.0086% to 3.1%, and their cumulative risk was 4.4%. Results indicated that the dietary risk of these fungicides in grapes was minor.

Residue transfer and risk assessment of carbendazim in tea.

BACKGROUND: Carbendazim (methyl 1H-benzimidazol-2-ylcarbamate) residue in tea is a public concern. The large gap in the maximum residue limits (MRLs) for carbendazim in tea makes it difficult to conduct pesticide management. Therefore, a systemic evaluation of the residue and the health risk of carbendazim from the tea garden to brewed tea was investigated. RESULTS: The dissipation of carbendazim in tea shoots followed first-order rate kinetics, with a half-life (t1/2 ) of 2.6 days. In green tea manufacturing, the carbendazim decrease of 2.81-26.74% was concentration-positive. The infusion factor of carbendazim was > 0.8 from dry tea to brewed tea and this was related to the brewing temperature and the size of the dry tea. All of the risk quotient values were significantly less than 1 in the evaluation of carbendazim residue in real tea samples and MRL. CONCLUSION: Residue transfer and risk assessment were evaluated for carbendazim in green tea. Carbendazim revealed a less persistent nature in tea plants. Green tea processing played a small role in decreasing carbendazim residue. Almost all of the carbendazim in dry tea leached into the brewed tea. However, the possible health risk induced by residual carbendazim in green tea was not significant. These findings are helpful when reconsidering the MRLs of carbendazim in tea. © 2018 Society of Chemical Industry.