Dissipation kinetics, residue determination and consumer risk assessment of acephate on green pea by gas chromatography-electron capture detector.

The present study on "acephate persistence on green pea" was conducted in SKUAST-Kashmir. The study aimed to determine the persistence, dissipation kinetics and waiting period of acephate on green pea. Acephate was sprayed at 75% soluble powder (SP) at 560 g a.i.ha-1 at the fruiting stage followed by another application at a 10 day interval. A rapid and accurate method (quick, easy, cheap, effective, rugged and safe, QuEChERS) was used for extraction and the residue was determined by gas chromatography-electron capture detection on a CPSIL-8CB capillary column (0.25um film thickness, 0.25 mm i.d, 30 m length). At the fortification levels of 0.05, 0.1 and 0.5 mg kg-1 , the percentage recovery of acephate on green pea was found in the range of 71-107%. The initial deposit of green pea was estimated to be 0.37 mg kg-1 . At the indicated dose, the residue of acephate on green pea dissipated below the limit of quantification of 0.05 mg kg-1 after 10 days. Acephate degradation was quick in green pea, with a half-life of 4.07 days. For safe eating of green peas, a 10 day waiting period is recommended. The gas chromatography-electron capture detection technique was validated by following the SANTE standards.

Health Risk Assessment of Pesticide Residues in Drinking Water of Upper Jhelum Region in Kashmir Valley-India by GC-MS/MS.

Globally growing demand for agricultural and farm foods has more or less become dependent on chemical pesticides to maintain the supply chain, which undoubtedly boosts agricultural production. However, pesticides not only impact the target pests but cause hazard to human health. Pesticides are ubiquitous and can be found in almost every component of the environment. They can therefore impair human and biota health when present over the threshold level. The present study assessed the concentration of commonly used pesticides for agricultural purposes but get mixed in different sources of water, as such fifteen sampling sites along the upper Jhelum basin of Kashmir valley were chosen. For the analysis, 60 water samples were obtained from different water sources. Gas chromatography coupled with tandem mass spectrometry (GC-MS/MS) was used to determine pesticide residues in water samples. Pesticide residues from 10 of the 26 commonly used pesticides were detected in water samples. Difenoconazole had the highest concentration among the pesticides detected, with a mean concentration of 0.412 ± 0.424 µg/L ranging from 0.0 µg/L to 0.8196 µg/L. The target hazards quotient (THQ) was used to quantify the possible noncarcinogenic health risks associated with drinking pesticide-contaminated water. Only chlorpyrifos and quinalphos were detected >1 in RWS3 (1.6571), RWS4 (1.0285), RWS14 (1.2571), and RWS15 (1.2000) sample sites, implying that the drinking water poses a health risk to humans. Hence, pesticide hazards should be mitigated and rigorous monitoring is needed to reduce pesticide residues in drinking water.

Risk assessment, development and validation of a GC-ECD-based method for the quantification of cypermethrin from green pea.

The present work describes the persistence, dissipation behaviour, half-life, risk assessment and novel gas chromatography method for the residue estimation of cypermethrin in green pea by spraying cypermethrin 10EC at 50 g a.i. ha-1 at fruiting stage followed by another application at a 10 day interval. The sample extraction and cleanup was followed bya modified quick, easy, cheap, effective, rugged, and safe method, and the residues of cypermethrin were determined using a validated gas chromatography method. The initial deposits were found to be 1.21 mg kg-1 following the application of insecticide at 50 g a.i. ha-1 . Cypermethrin residues declined to below the detection limit of 0.05 mg kg-1 after 15 days at the recommended dosage. The half-life of cypermethrin was 2.66 days at 50 g a.i. ha-1 . For risk assessment studies, the waiting period of 15 days is recommended as safe for consumption for the insecticide. The GC-ECD method was validated according to the SANTE guidelines by various analytical parameters including linearity, accuracy, detection and quantification limits. The developed method is simple, selective and repeatable, and can be used for the standardization of pesticides on fruits and vegetables.

Risk assessment, dissipation behavior and persistence of quinalphos in/on green pea by gas chromatography with electron capture detector.

Chemical investigation was carried out to examine the risk assessment, dissipation behavior, persistence, and half-life period of quinalphos in/on green pea fruit by spraying quinalphos at fruiting stage followed by another application after 10-day interval. The samples were extracted by using the quick, easy, cheap, effective, rugged, and safe method, and the residues of quinalphos were analyzed by gas chromatography with electron capture detector. Herein, we report a novel, accurate, and cost-effective gas chromatography method for the determination of average deposits of quinalphos in/on green pea. The initial deposits and half-life of quinalphos were found to be 1.20 mg/kg and 2.77 days, respectively, following the application of insecticide. Residues of quinalphos reached below detection limit of 0.05 mg/kg after 10 days at recommended dosage. For risk assessment studies, the tenth day will be safe for consumers for consumption of green pea. The developed method is simple, selective, and repeatable, and it can be extended for quinalphos-based standardization of herbal formulations containing green pea and its use in pesticide industries.

Quantification, dissipation behavior and risk assessment of ethion in green pea by gas chromatography-electron capture detector.

Residue investigation was carried out to scrutinize the persistence, dissipation behavior, half-life, and risk assessment of ethion on green pea fruit by spraying ethion at the fruiting stage followed by another application at 10 day intervals. The samples were extracted by using a quick, easy, low-cost, effective, rugged, and safe method, and the residues of ethion were analyzed by gas chromatography with electron capture detection. Here we report a novel, accurate, and cost-effective gas chromatography method for the determination of average deposits of ethion on green pea. The initial deposits were found to be 4.65 mg/kg following the application of insecticide. Residues of ethion reached below the detection limit of 0.10 mg/kg after 25 days at recommended dosage. The half-life of ethion was found to be 4.62 days. For risk assessment studies, the 25th day will be safe for consumers for the consumption of green peas. The developed method is simple, sensitive, selective, and repeatable and can be extended for ethion-based standardization of herbal formulations containing green pea and its use in pesticide industries.

Isolation, cytotoxic evaluation, and simultaneous quantification of eight bioactive secondary metabolites from Cicer microphyllum by high-performance thin-layer chromatography.

Chemical investigation of Cicer microphyllum resulted in the isolation and characterization of eight natural products viz. Stigmasterol, Oleanolic acid-3-acetate, Oleanolic acid, Biochanin A, Genistein, Pratensein, Chrysoeriol, and Luteolin. Herein, we report a novel, accurate, and cost-effective high-performance thin-layer chromatography method for the simultaneous quantification of the isolated natural products on silica-gel 60F254 plates using the solvent system n-hexane/ethyl acetate/formic acid (9.0:6.5:0.8, v/v/v). Natural products were quantified after postchromatographic derivatization with ceric ammonium sulfate. The method was validated as per the International Conference on Harmonization guidelines. All calibration curves showed a good linear relationship (r > 0.9943) within the test range. Precision was assessed by intra- and interday tests with relative standard deviations <1.82%, accuracy validation recovery 98.38-99.57% with relative standard deviations <1.00%. On quantification, Pratensein was a major constituent (0.921%). The screening for cytotoxic activity using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay resulted into identification of Luteolin as potent molecule with IC50 3.5 and 25.6 µg/mL against murine melanoma and human epidermoid carcinoma cell lines, respectively.