Assessment of protein adhesion behaviour and biocompatibility of magnesium/Co-substituted HA-based composites for orthopaedic application.

Protein adsorption has a great influence on Mg-based metallic implants, which affects cell attachment and cell growth. Adsorption of the proteins (via electrostatic interaction, hydrophobic/hydrophilic, and hydrogen-bonding) on the implant surface is greatly influenced by the surface chemistry of the implant. Hydroxyapatite (HA) is a class of CaP ceramic, beneficial for protein adsorption as it possesses Ca2+ and PO43- in it, which are believed to be the protein binding sites on the HA surface. Moreover, HA is the popular choice for reinforcement in the magnesium matrix owing to its similarity with bone mineral composition. However, negligible interaction between HA and Mg particles during sintering is the major limitation for frequent usage of Mg-HA implants. Doping of HA with Mg2+ and Zn2+ (CoHA) ions leads to its chemistry similar to natural apatite in human bone and facilitates comparatively better bonding with the MgZn matrix. This study mainly aims to delve into the protein adsorption behaviour of Magnesium/Co-substituted HA-based Composites (M3Z-CoHA) along with their biocompatibility. Qualitative and quantitative protein adsorption analysis shows that the addition of 15 wt% CoHA to Mg matrix enhanced protein adsorption by ~60% and renders cell viability >90% after day 1, supporting cellular growth and proliferation. The implants also initiated osteogenic differentiation of the cells after day 7. The leached-out products from all the composites showed no toxicity. The morphology of the cells in all the composites was found as healthy as the control cells. Overall, the composite with 15 wt% HA reinforcement (M3Z-15CoHA) has shown favourable protein adsorption behaviour and cytocompatibility.

Assessment of biomechanical stability and formulation of a statistical model on magnesium based composite in two different milieus.

Magnesium (Mg) based temporary implants are an appealing new solution to counter the problems associated with the currently available temporary orthopaedic implants, used in fracture fixing. To make the extensive use of Mg-based implants in-vivo, mechanical integrity in the physiological environment is a prerequisite. This study presents an insight into the biomechanical stability of Mg-3Zn/HA (0, 5, and 15 wt % of HA) composites in two different milieus (simulated body fluid (SBF) and serum contained SBF (m-SBF)). After 14 days of static immersion in SBF, ~65% mechanical strength was compromised in the case of 15 wt % HA reinforcement. However, the degradation rate was slowed down by ~35% with the addition of 15 wt % HA in Mg-3Zn. Mg-3Zn/HA composite, when soaked in both fluids, was found to induce apatite layer formation on the surfaces for several days. However, in the case of m-SBF immersion, 15 wt % HA facilitated less precipitation of apatite growth when compared to SBF immersion. Nevertheless, m-SBF immersed 15 wt % HA composite facilitated better corrosion resistance and excellent mechanical stability after 14 days of immersion. The approach thereby assists in establishing an effective mechanism between the degradation and mechanical stability in in-vitro immersion. In addition, this study has also developed a semi-empirical model for prediction of the compressive strength of these composites as a function of the number of days of immersion and the content of hydroxyapatite (HA). This semi-empirical model will help in predicting the biomechanical stability for long-term in-vitro exposures, which might be of use in evaluating the effect of the in-vivo environment.