Histologically validated scoring system for the assessment of hock burn in broilers.

The assessment of bird-based welfare indicators plays an important role in the evaluation of bird welfare. The aim of the study was to histologically validate a visual scoring system for hock burn in broilers and to detect threshold values of a visual score to define welfare-relevant alterations in terms of mild lesions or ulcers of the hock. We collected 200 hocks of 39- to 42-day-old Ross 308 broilers after the slaughter process. Each hock was scored visually ("macro scores" 0-4) and evaluated histologically ("micro scores" 0-3), with high scores representing more severe lesions. Although we found a tendency for higher micro scores with increasing macro scores, an exact allocation of macro to micro scores was not possible. For example, macro score 1 could represent micro scores 1, 2 and 3, whereas macro scores 3 and 4 always represented micro score 3 (ulcer). The conditional probability of certain micro scores for given macro scores was estimated using a multinomial logistic regression model. Ulcer showed the highest probability at macro score 1, whereas mild lesions were not found to have an estimated highest probability at any macro score. The depth of inflammation of hock burn lesions increased with increasing macro scores up to macro score 3 with an average depth of 1019 µm. Visually more severe and deeper lesions were also histologically rated with higher scores. Thus, considering limitations, the herein validated macroscopic assessment scheme for hock burn allows an estimation of histological alterations in hocks of broilers.RESEARCH HIGHLIGHTS Histological validation of a visual assessment scheme for hock burn in broilers.Tendency for higher micro scores with increasing macro scores.Estimation of histological score via macro score possible with limitations.Histological depth of inflammation increased with an increasing macro score.

Simulation of neutron production using MCNPX+MCUNED.

In standard MCNPX, the production of neutrons by ions cannot be modelled efficiently. The MCUNED patch applied to MCNPX 2.7.0 allows to model the production of neutrons by light ions down to energies of a few kiloelectron volts. This is crucial for the simulation of neutron reference fields. The influence of target properties, such as the diffusion of reactive isotopes into the target backing or the effect of energy and angular straggling, can be studied efficiently. In this work, MCNPX/MCUNED calculations are compared with results obtained with the TARGET code for simulating neutron production. Furthermore, MCUNED incorporates more effective variance reduction techniques and a coincidence counting tally. This allows the simulation of a TCAP experiment being developed at PTB. In this experiment, 14.7-MeV neutrons will be produced by the reaction T(d,n)(4)He. The neutron fluence is determined by counting alpha particles, independently of the reaction cross section.

[Behaviour therapy in the practice management of a veterinary practice. Survey among practicing veterinarians and veterinarians specializing in behaviour therapy]. / Verhaltenstherapie im Praxismanagement einer Tierarztpraxis. Befragung praktischer und auf Verhaltenstherapie spezialisierter Tierärzte.

OBJECTIVE: of the study was to investigate whether behaviour therapy may be an economic niche within practice management. MATERIAL AND METHODS: A questionnaire was used to analyze to what extent veterinarians have already applied behaviour therapy (BT) and how they evaluate economic efficiency and patient owners' attitude. The descriptive analysis included the data from 312 practicing veterinarians (167 randomly selected, interviewed and 145 signed on for training sessions, veterinarians) and of 23 veterinarians specialized in BT. RESULTS: Two-thirds (67% of n=288) of the practicing veterinarians offered BT in their practices. The economic efficiency of BT was evaluated as positive by 64% (of n=281) of the practicing veterinarians and by 83% (of n=23) of specialists. 32% (of n = 146) of practitioners who offered behavioural therapy confirmed an increase in sales through the application of BT. Among the specialists, 84% (of n=19) confirmed this experience. In comparison to the specialists and literature data, most of the practicing veterinarians spent insufficient time (61% of n=180) for a behaviour consultation. Furthermore, most of them (86% of n=162) charged less for BT than the amounts fixed by the German Payment Regulations (Gebührenordnung für Tierärzte), as compared to the specialists. CONCLUSION: The specialized veterinarians offered, in contrast to most practitioners, a consultation of BT with sufficiently calculated time and accurate payment planning to realise a therapeutic and commercial outcome. Therefore, the assessment of the economic efficiency of BT and their sales increase through BT were better than the estimation of the practicing veterinarians. Behaviour therapy cannot be managed by providing advice free of charge or charging less. Veterinarians have to become aware that BT is a veterinary area of specialization for which an adequate qualification is necessary. If the veterinarian is not specialized in BT he should refer to a qualified colleague.

Assessment of colostral transfer and systemic availability of immunoglobulin G in new-born foals using a newly developed enzyme-linked immunosorbent assay (ELISA) system.

To measure the immunoglobulin G (IgG) concentration in colostrum, milk and serum samples, a sandwich enzyme-linked immunosorbent assay (ELISA) detection system was developed. The system provided high reproducibility and sensitivity for routine diagnostic purposes. The period of fluctuating serum concentrations of IgG was monitored in new-born foals and their mares for a period of 6 weeks postnatum and postpartum, respectively. All foals received colostrum from their mares. The mean IgG concentration in the precolostral mare serum was approximately 19.0 mg/ml and decreased significantly to 13.8 mg/ml within the first 24 h postpartum. The IgG value fell to a minimum of 11.2 mg/ml by day 21 and increased to 21.6 mg/ml by day 42 postpartum. Within the first 4 h postpartum, mean IgG concentrations of 54.5 mg/ml were measured in the colostrum. A significant decrease to 10.1 mg IgG/ml colostrum was then noted 9-12 h postpartum. The mean IgG concentrations in foal serum increased from 0.3 mg/ml (precolostral value) to 9.6 mg/ml within 5-8 h postnatum. After 13-16 h postnatum, the highest IgG value of 15.7 mg/ml was reached. Over time the mean IgG concentration decreased significantly to 7.9 mg/ml at day 35. At the end of the observation period (day 42 postnatum) the mean IgG concentration once again increased to 11.2 mg/ml serum. In addition, the possible influence of various parameters on IgG concentration were examined. No significant influences could be shown by the breed, mare age, number of pregnancies, days of gestation, month foaled, foal sex, or the different farms. Finally, the cumulative incidence of failure of passive transfer (FPT) defined as IgG levels < 4 mg/ml foal serum, and partial FPT (PFPT) at levels ranging from 4 to 8 mg/ml foal serum was determined. From a total of 70 foals, 10.0% showed FPT and 18.6% showed PFPT.