RESUMO
The characterization of impurities present in micronomicin sulfate injection by liquid chromatography (LC) coupled with mass spectrometry (MS) is described. A reversed phase (RP)-LC method using a C18 column resistant to an alkaline (pH 11) aqueous mobile phase was developed and coupled to MS with an electrospray ionization (ESI) source in the positive ion mode which provides MS(n) capability. A total of thirty six impurities were detected in commercial samples: five impurities were identified by comparison of their fragmentation patterns with those of available related substances, eleven of them were identified in accordance with relevant literature, while the other twenty impurities were newly identified using the MS/MS spectra of the available related reference substances as interpretative templates combined with knowledge of the nature of functional group fragmentation behaviors. This work was applied to evaluate the quality of micronomicin sulfate injection from different manufacturers.
Assuntos
Aminoglicosídeos/química , Antibacterianos/química , Contaminação de Medicamentos , Aminoglicosídeos/administração & dosagem , Aminoglicosídeos/economia , Antibacterianos/administração & dosagem , Antibacterianos/economia , China , Cromatografia Líquida de Alta Pressão , Cromatografia de Fase Reversa , Gentamicinas , Injeções , Estrutura Molecular , Controle de Qualidade , Espectrometria de Massas por Ionização por Electrospray , Ésteres do Ácido Sulfúrico/administração & dosagem , Ésteres do Ácido Sulfúrico/química , Ésteres do Ácido Sulfúrico/economia , Espectrometria de Massas em TandemRESUMO
OBJECTIVE: To analyze LC-MS fingerprints of Aristolochia manshuriensis for quality assessment with two different chemical pattern recognition models. METHOD: LC-MS fingerprints of A. manshuriensis were established from 24 batches of samples from different habitats. SIMCA and Clustering analysis were used to compare the parameters of the 29 common peaks. RESULT: Two methods had good consistency, while they reflected the inherent sample information from different perspectives, respectively. CONCLUSION: Modern equipment analysis technology and multivariable chemical pattern recognition would be an efficient way for quality control and variety identification of A. manshuriensis.