RESUMO
Aflatoxins (AFs) are secondary metabolites mainly produced by Aspergillus flavus and A. parasiticus and their contamination of red peppers can cause hepatocellular carcinoma, growth retardation in children, immune suppression, and death. In addition, their presence in the red peppers can affect international trade and cause significant economic burdens. Thus, the objective of this study was to assess the level of AFs contamination in packed powder (from supermarkets) and raw red pepper samples commercially available in the towns of Fiche and Mukaturi. Furthermore, this study aimed to determine the potential health and cancer risks associated with the consumption of red pepper contaminated with AFs. Red pepper samples (raw and packed powder) were collected randomly from the Fiche and Mukaturi open markets. Then AFs in the samples were extracted using methanol: water (80:20, v/v). These extract samples were then cleaned up using an immunoaffinity column (IAC) and determined with a high-performance liquid chromatography-fluorescence detector (HPLC-FLD). The finding showed that the amount of AFB1, AFB2, and AFG1 in raw red pepper was found to be 3.19 ± 0.01, 0.19 ± 0.001, and 4.07 ± 0.01 µg kg-1, respectively. The raw red pepper samples had a total of 7.66 ± 0.01 µg kg-1 of AFs. On the other hand, the amount of AFB1, AFB2, and AFG1 in Afiya-packed red pepper was found to be 7.04 ± 0.03, 2.15 ± 0.06, and 0.50 ± 0.01 µg kg-1, while Mudayi packed red pepper contained 31.60 ± 0.22, 24.40 ± 0.17, 3.37 ± 0.02 and 2.48 ± 0.004 µg kg-1 of aflatoxins, respectively. Afiya and Mudayi packed powder peppers had a total AFs content of 10.4 ± 0.07 and 61.90 ± 0.28 µg kg-1, respectively. The total AFs concentrations in packed pepper powder samples were higher than maximum toleratable limits (MTLs) set by the European Commission Regulation (EU) 2023/915 (5.00 µg kg-1 for AFB1 and 10 µg kg-1 for total AFs). AFB1 (31.60 ± 0.22 µg kg-1) had the highest level of contamination, followed by AFB2 (24.40 ± 0.17 µg kg-1) in packed pepper powder. In the adult population, the estimated daily intake (EDI) of AFB1, AFB2, AFG1, and AFG2 ranged from 0.80 to 7.90, 0.04 to 6.10, 0.02 to 1.02, and 0.05 to 0.62 µ g kg-1 body weight (bw) per day, respectively. However, the Margins of Exposure (MOE) values and combined Margin of Exposure (MoET) for these chemicals were significantly lower than the safe margin (<10 000). Therefore, this study highlights the potential health risks associated with consuming AFs-contaminated red peppers and the need for stricter regulations and monitoring to ensure food safety.
RESUMO
Cow milk is a complete and highly nutritious source of food for humans. However, the quality of milk products has become a significant health concern for consumers, particularly infants and children, in many developing nations, including Ethiopia. The objective of this study was to determine the heavy metal levels in raw cow milk collected from dairy producers and collection centers in Mukaturi town, Ethiopia. Sixty raw cow milk samples (40.0 milk samples from dairy farms and 20.0 milk samples from collection centers) were randomly collected and digested using a mixture of nitric acid (HNO3), hydrogen peroxide (H2O2) and perchloric acid (HClO4) on a hot plate. The amounts of heavy metals (Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, and Zn) in the milk samples were determined using inductively coupled plasma atomic emission spectroscopy (ICP-OES). The findings showed that the average concentrations of Pb (2.31 ± 0.127), Fe (0.566 ± 0.130), Ni (0.210 ± 0.0189), Cd (0.0372 ± 0.0230), Cr (0.369 ± 0.0162), and Co (0.225 ± 0.0150) in mgL-1 were higher than the allowable limits. This could pose a health risk to the public. However, the concentrations of Mn (0.044 ± 0.0369), Cu (0.195 ± 0.0450) and Zn (2.90 ± 0.0570) in mg L-1 were lower than or within the recommended limits and cannot pose any threat to consumers. The validity of the digestion processes was checked by the recovery test. The percentage recoveries of Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, and Zn were found to be in the range of 80.7-122 %, which is within the acceptable range. Therefore, special attention should be given to the continuous monitoring of heavy metal levels in raw milk among dairy producers and collection centers to minimize economic losses and the risk to consumer safety.