Enzymatic synthesis of lipophilic lutein-PUFA esters and assessment of their stabilization potential in EPA-DHA rich fish oil matrix.

The objective of the present study was to synthesize ω-3 polyunsaturated fatty acid esters of lutein and to evaluate if esterification can stabilize the both bioactive molecules. Both ω-3 polyunsaturated fatty acid and lutein are prone towards auto-oxidation in their free form. Free lutein extracted from the marigold petals was enzymatically esterified using Candida antarctica NS435 Lipase B, with the ω-3 long-chain polyunsaturated fatty acids. The lutein esters were purified, characterized and finally assessed for their protective role against oxidative degradation in bulk fish oil matrix. The antioxidative effect of these esters was compared with commercial antioxidants of natural origin, i.e., α-tocopherol and a synthetic antioxidant, i.e., tert-butylhydroquinone, at a dosage of 200 mg/L. Both free lutein and lutein-polyunsaturated fatty acid ester had significantly promoted the oxidative stability of bulk fish oil. But based on dose-response relationship, lutein-polyunsaturated fatty acid ester was found to be more efficient than free lutein, in protecting fish oil from secondary oxidation, thereby augmenting their shelf life. Given the high nutraceutical value, potent antioxidative potential and organic origin, it is only relevant to incorporate lutein esters as natural preservative and stabilizers in edible oils.

Studies on the fluidity of milk lipids of mothers from three socioeconomic groups of West Bengal, India.

This study evaluated the fluid character of human milk by determining the mean melting points (MMP) of fatty acids of milk lipid of Bengali mothers. Fatty acid methyl esters were analyzed by gas liquid chromatography. MMPs were calculated from the fatty acid concentration (% w/w) and their molar mass. Phospholipid content of samples was also determined. The MMPs of milk lipid of higher income group (n = 48), medium income group (n = 57) and lower income group (n = 112) mothers were found to be 31.33°C ± 0.61, 36.86°C ± 0.64 and 35.11°C ± 0.65, respectively, which showed a significant correlation with the fatty acid composition, with P < 0.0001, 0.003, 0.0001, respectively. The average MMP of milk lipid (34.43°C ± 0.63) of these three groups was below the core body temperature (37.4°C) of human beings, which perhaps helps in maintaining the milk fluidity as well as lipid digestion in breastfed infants.