Technical and clinical assessment of the use of a new material-based splint in orthognathic surgery.

Heat or auto-cured acrylic resins are materials that are commonly used in splint construction. Newly developed light-cured resins hold promise in view of the altered characteristics of the material. The aim of this study was to evaluate how far light-curing resin-based splints can be used in orthognathic surgery. Over a period of 1 year, 141 orthognathic surgeries were planned and performed using randomly chosen light-cured splints versus auto-polymerized splints. The performance of splint fabrication, the model planning and the clinical use were assessed by different measurements. The dental technician, the orthodontist and the surgeon evaluated objective parameters (model damage, fabrication time, accuracy of registration, adjustment time) as well as subjective criteria (handling). This study revealed that the use of light-curing splints significantly improved the accuracy (97.7% versus 81.2% in the case of acrylic materials). Less time was needed for the fabrication and adjustment of the splint. There was concomitantly less model damage to be observed when light-curing resins were used (9.2% versus 83.5%) and subjective measurements revealed significantly better handling of light-curing resin-based splints. Light-curing resin splints used in orthognathic surgery seem to be advantageous.

[Experimental and finite-element models for the assessment of mandibular deformation under mechanical loading]. / Experimentelle und Finite-Elemente-Analyse der Biomechanik des Unterkiefers unter Belastung.

Biomechanical investigations of the mandible are difficult to perform due to a variety of conditions involved. For the appropriate reconstruction of biomechanical properties, a geometrically correct body model has to be established which fits to complex in vivo conditions. The aim of our study was to evaluate the use of finite-element models (FEM) for the assessment of mandibular deformation under mechanical loading. Explanted human mandibles (n = 5) were investigated by strain gauges to determine the individual strain distribution under mechanical loading. FEM analysis based on a computed tomograph (CT) was performed and the results were matched with the test data. Our study demonstrates only minor interindividual differences in the strain distribution for each load studied. The mechanical response in terms of deformation was found to depend mainly on gross geometrical properties and to a minor extent on the various other variables. At all positions the maximum principal strain was tensile, the minimum principal strain was compressive, and the absolute strain values were correlated with the magnitude of the applied force. CT-based FEM analysis revealed the utility of mathematical models to approximate simulated data our experimental results. Hence, FEM analysis is a non-invasive tool in the prediction of biomechanical behaviour of individual mandibles and therefore may help in trauma reconstruction and treatment planning.

Heart transplants--assessment of dental procedures.

The object of this study was to evaluate the effects of dental foci on survival rates and rejection episodes in heart transplant recipients. Therefore, in a retrospective longitudinal study we studied 74 heart transplant recipients at the Department of Maxillofacial Surgery and Department of Thoracic and Cardiovascular Surgery, University of Münster. Study patients were divided into groups: those in which dental foci had been verified (n = 31) and those without dental foci (n = 43). Statistical analysis was performed using the chi-square test, Kaplan-Meier life table analysis, and the log-rank test. Before heart transplantation, patients were screened clinically and radiographically to determine the extent of dental foci. Postoperatively, patients were evaluated dentally and medically to identify the impact of dental foci on the incidence of systemic and oral infections, frequency and severity of rejection episodes, mortality, and complications arising during dental treatment. By comparing the mortality, infection and rejection rates in the various groups no statistically significant differences (P > 0.05) were found between patients. Despite immunosuppression, extended inflammatory processes such as abscess formation or viral stomatitis were not found in the oral cavity. We therefore suggest that patients suffering from the symptoms of severe heart failure need not be subjected to rigorous preoperative dental treatment.

Molecular assessment of p53 abnormalities at the invasive front of oral squamous cell carcinomas.

BACKGROUND: The prognostic significance of the invasive tumor front in squamous cell carcinomas has recently been recognized. The aim of the present study was to investigate possible molecular mechanisms underlying the significance of this area in oral squamous cell carcinomas. METHODS: We used immunohistochemical and molecular genetic techniques to investigate whether p53 alterations at the invasive tumor front could determine the aggressiveness of oral cancers. RESULTS: p53 Overexpression was detected in 52% to 56% (four different p53 antibodies) of 100 carcinomas studied. The concordance rate between results of immunohistochemistry and genetic analysis was 60%. No correlation was found between p53 status of the tumors and clinicopathologic parameters analyzed statistically. CONCLUSIONS: p53 Alterations have no prognostic impact in oral squamous cell carcinomas and apparently do not represent a molecular basis for the biologic significance of the invasive tumor front. The detection of discordant p53 aberrations between primary and second primary carcinomas in some patients provide evidence for their independent origin, with possible impact on prevention and therapy.

Ultrasonographic versus conventional diagnostic procedures in dislocated subcondylar mandibular fractures.

In patients with severe craniomaxillofacial trauma, immediate attention is directed primarily to the early diagnosis of cervical spine injuries, intracranial injuries, respiratory difficulties, and vascular compromises. Diagnostic studies, such as computed tomography scans, are sometimes not useful for the evaluation of mandibular fractures, since cuts are performed too superiorly. The plane radiographs obtained in emergency settings are frequently of minimal diagnostic value. Fractures of the mandibular symphysis, body, and angles are easily identified clinically; subcondylar fractures, however, are not directly accessible for clinical examination. The evaluation of suspected dislocated subcondylar fractures with the aid of ultrasonography is reliable, highly sensitive and specific, and cost-effective.

In situ assessment of cell proliferation at the invasive front of oral squamous cell carcinomas.

In oral squamous cell carcinoma (OSCC) the histopathological malignancy grading of the invasive front has been found to offer the most reliable prognostic parameter. In the present study we compared such tumour front grading of 100 OSCCs with the in situ growth fraction demonstrated by MIB1 immunostaining following wet autoclave antigen retrieval. MIB1 labelling indices (LIs) were estimated both at the invasive front and in the central parts of OSCCs using two different evaluation methods (overall and random counting) to investigate whether MIB1 LIs represent a possible biological background for the tumour front grading. Statistically highly significantly increased MIB1 LIs were found at the invasive tumour fronts with both counting methods compared with the centres of the same tumours. For LI estimation the classic overall counting procedure proved to be superior. However, in contrast to tumour front grading, MIB1 LIs revealed no correlation with the clinical outcome of the patients concerned. Our results demonstrate that the invasive tumour front of an OSCC is composed of (a) tumour subpopulation(s) with higher proliferative activity. However, determination of the proliferative activity by MIB1 of this tumour area offers no prognostic information.