The state of fish parasite discovery and taxonomy: a critical assessment and a look forward.

Efforts to find and characterise new parasite species in fish hosts are crucial not just to complete our inventory of Earth's biodiversity, but also to monitor and mitigate disease threats in fisheries and aquaculture in the face of global climate change. Here, we review recent quantitative assessments of research efforts into fish parasite discovery and taxonomy. We address broad questions including: Are efforts aimed at finding new parasite species targeted at geographical hotspots of fish biodiversity, where there should be more parasite species to be found? Is the application of molecular tools to study parasite genetic diversity deployed strategically across regions of the world, or focused disproportionately on certain areas? How well coordinated is the search for new parasite species of fish among workers specialising on different higher helminth taxa? Are parasite discovery efforts in any geographical area consistent over time, or subject to idiosyncrasies due to the waxing and waning of highly prolific research careers? Is the quality of taxonomic descriptions of new species improving over time, with the incorporation of new tools to characterise species? Are taxonomic descriptions moving away from a focus on the adult stage only toward attempts to characterise the full life cycle of newly-discovered helminth species? By using empirical evidence to answer these questions, we assess the current state of research into fish parasite discovery and taxonomy. We also explore the far-reaching implications of recent research on parasite microbiomes for parasite taxonomy. We end with recommendations aimed at maximising the knowledge gained per fish sacrificed, and per dollar and time invested into research on fish parasite biodiversity.

A new species of Spauligodon (Nematoda: Oxyurida: Pharyngodonidae) in geckos from São Nicolau Island (Cape Verde) and its phylogenetic assessment.

A new nematode species, Spauligodon nicolauensis n. sp., is described from geckos Tarentola bocagei and Tarentola nicolauensis on the island of São Nicolau, Cape Verde. The new nematode was found in the pellets obtained directly from the geckos in a non-invasive fashion, and its identity was assessed both at morphologic and genetic levels. The new species has morphological similarities with Spauligodon tarentolae Spaul, 1926, also parasitizing geckos from the Canary Islands. However, the male cloacal region in the new species is distinct, presenting a different shape of the caudal papillae. The overall resemblance probably resulted from colonization via descent from an ancestor of S. tarentolae carried by the ancestor of Cape Verde Tarentola. The analysis of nuclear DNA sequences confirms that the new species is phylogenetically distinct from all other Spauligodon species already analyzed, forming a group clearly separated from species parasitizing lacertid lizards. The COI genetic distance suggests that the S. nicolauensis n. sp. found in the 2 species of geckos in São Nicolau Island may have resulted from a host-switching event, when they came into contact after the unification of the island.

A phylogenetic assessment of the colonisation patterns in Spauligodon atlanticus Astasio-Arbiza et al., 1987 (Nematoda: Oxyurida: Pharyngodonidae), a parasite of lizards of the genus Gallotia Boulenger: no simple answers.

Parasite taxonomy traditionally relies on morphometric and life-cycle characteristics which may not reflect complex phylogenetic relationships. However, genetic analyses can reveal cryptic species within morphologically described parasite taxa. We analysed the phylogenetic variation within the nematode Spauligodon atlanticus Astasio-Arbiza, Zapatero-Ramos, Ojeda-Rosas & Solera-Puertas, 1987, a parasite of the Canarian lizard genus Gallotia Boulenger, inferring the origin of their current association. We also attempted to determine its relationship with other Spauligodon spp. Three different markers, mitochondrial COI plus nuclear 18S and 28S ribosomal RNA, were used to estimate the evolutionary relationships between these nematodes. S. atlanticus was found to be paraphyletic, suggesting that Gallotia spp. were colonised by two independent lineages of Spauligodon. Additional analyses of other Spauligodon spp. are required for a more complete interpretation of the evolution of this genus from the Canarian archipelago and its closest taxa. Our results emphasise the importance of extensive sampling and phylogenetic studies at the intrageneric level, and highlight the limitations of a morphologically based taxonomy in these parasites.