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1.
Plant Physiol Biochem ; 199: 107745, 2023 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-37172402

RESUMO

BACKGROUND: Nanotechnology offers many benefits in the globally important field of food production and human nutrition, particularly by implementing agricultural nanoproducts. Of these, edible plant fertilizers enriched with nanosized forms of essential metals, Mn and Fe, are growing in importance with the advantages of enhanced action on plant roots. SCOPE AND APPROACH: This review focuses on the importance of tracking the bioaccumulation and biodistribution of these pertinent nanofertilizers. An emphasis is given to the critical analysis of the state-of-the-art analytical strategies to examine the Mn and Fe nanoparticles in edible plant systems as well as to shedding light on the vast gap in the methodologies dedicated to the speciation, in vitro simulation, and safety testing of these promising nanomaterials. Also provided are guidances for the food chemists and technologists on the lights and shadows of particular analytical approaches as a matter of authors' expertise as analytical chemists. KEY FINDINGS AND CONCLUSIONS: While the use of nanotechnology in agriculture seems to be growing increasingly, there is still a lack of analytical methodologies capable of investigating novel Mn- and Fe-based nanomaterials as potential fertilizers. Only the advent of reliable analytical tools in the field could bridge the gaps in our knowledge about processes in which those materials participate in the plant systems and their effects on crop production and quality of the produced food.


Assuntos
Fertilizantes , Plantas Comestíveis , Humanos , Fertilizantes/análise , Manganês , Distribuição Tecidual , Agricultura/métodos , Medição de Risco , Nanotecnologia/métodos
2.
Metallomics ; 11(12): 2010-2019, 2019 12 11.
Artigo em Inglês | MEDLINE | ID: mdl-31593199

RESUMO

The scientific interest in cadmium (Cd) as a human health damaging agent has significantly increased over the past decades. However, particularly the histological distribution of Cd in human tissues is still scarcely defined. Using inductively coupled plasma-mass spectrometry (ICP-MS), we determined the concentration of Cd in 40 different human tissues of four body donors and provided spatial information by elemental imaging on the microscopic distribution of Cd in 8 selected tissues by laser ablation (LA)-ICP-MS. ICP-MS results show that Cd concentrations differ by a factor of 20 000 between different tissues. Apart from the well know deposits in kidney, bone, and liver, our study provides evidence that muscle and adipose tissue are underestimated Cd pools. For the first time, we present spatially resolved Cd distributions in a broad panel of human soft tissues. The defined histological structures are mirrored by sharp cut differences in Cd concentrations between neighboring tissue types, particularly in the rectum, testis, and kidneys. The spatial resolution of the Cd distribution at microscopic level visualized intratissue hot spots of Cd accumulation and is suggested as a powerful tool to elucidate metal based toxicity at histological level.


Assuntos
Tecido Adiposo/química , Osso e Ossos/química , Cádmio/análise , Rim/química , Fígado/química , Músculos/química , Tecido Adiposo/metabolismo , Animais , Osso e Ossos/metabolismo , Cádmio/farmacocinética , Humanos , Rim/metabolismo , Fígado/metabolismo , Masculino , Espectrometria de Massas/métodos , Músculos/metabolismo , Reto/química , Reto/metabolismo , Reprodutibilidade dos Testes , Testículo/química , Testículo/metabolismo , Distribuição Tecidual
3.
J Pharm Biomed Anal ; 174: 300-304, 2019 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-31195318

RESUMO

Along with a growing interest in biomedical applications of metal-based nanoparticles, there is a compelling need in systematic information on their behavior in human body systems, preferably at the cellular level. However, in most of the in-vitro uptake experiments, the nanomaterial was applied in its native form that in reality can hardly reach the cell. In this work, we developed an improved procedure in which prior to addition to the cells the particles are converted into the protein conjugates by incubation in human serum. The procedure was tested for gold nanoparticles of different size, chosen as a representative nanomaterial on multifunctional medicinal use, and MCF-7 cell line. Using ICP-MS to measure intracellular metal concentration, it was shown that an original state has significant effect on particle internalization. The protein corona significantly inhibits the uptake amount by MCF-7 cells, with the greatest influence (a 15-fold decrease compared to uncoated particles) being exerted over the smallest, 5-nm particles (3 pg Au/cell). Conjugates of larger particles (20 and 50 nm) are taken up more effectively (45 and 34 pg Au/cell, respectively). The advanced protocol makes the uptake results more reliable and its implementation may accelerate the preclinical development of metal-based nanoparticles as a viable theranostic implement.


Assuntos
Ouro/farmacocinética , Espectrometria de Massas/métodos , Nanopartículas Metálicas/química , Coroa de Proteína , Adsorção , Linhagem Celular Tumoral , Humanos , Células MCF-7 , Micro-Ondas , Tamanho da Partícula , Reprodutibilidade dos Testes
4.
Anal Bioanal Chem ; 410(27): 7211-7220, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30155703

RESUMO

Quantitative screening for potential drug-protein binding is an essential step in developing novel metal-based anticancer drugs. ICP-MS approaches are at the core of this task; however, many applications lack in the capability of large-scale high-throughput screenings and proper validation. In this work, we critically discuss the analytical figures of merit and the potential method-based quantitative differences applying four different ICP-MS strategies to ex vivo drug-serum incubations. Two candidate drugs, more specifically, two Pt(IV) complexes with known differences of binding affinity towards serum proteins were selected. The study integrated centrifugal ultrafiltration followed by flow injection analysis, turbulent flow chromatography (TFC), and size exclusion chromatography (SEC), all combined with inductively coupled plasma-mass spectrometry (ICP-MS). As a novelty, for the first time, UHPLC SEC-ICP-MS was implemented to enable rapid protein separation to be performed within a few minutes at > 90% column recovery for protein adducts and small molecules. Graphical abstract Quantitative screening for potential drug-protein binding is an essential step in developingnovel metal-based anticancer drugs.


Assuntos
Antineoplásicos/metabolismo , Proteínas Sanguíneas/metabolismo , Compostos Organoplatínicos/metabolismo , Antineoplásicos/análise , Proteínas Sanguíneas/análise , Cromatografia em Gel/métodos , Cromatografia Líquida de Alta Pressão/métodos , Análise de Injeção de Fluxo/métodos , Humanos , Espectrometria de Massas/métodos , Metais/análise , Metais/metabolismo , Compostos Organoplatínicos/análise , Ligação Proteica , Ultrafiltração/métodos
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