RESUMO
A highly specific test system based on polyclonal rabbit antibodies targeting a conjugate of alternariol and bovine serum albumin prepared by formaldehyde condensation has been developed for the first time and shown to have the sensitivity of 0.4 ng/ml in the indirect competitive determination of alternariol. The possibility of applying the assay for assessment of the intensity of alternariol contamination of corn, animal feed, and raw materials used for the preparation of the latter has been demonstrated.
Assuntos
Anticorpos/metabolismo , Haptenos/análise , Técnicas Imunoenzimáticas/métodos , Lactonas/análise , Alternaria/metabolismo , Ração Animal/análise , Animais , Ligação Competitiva , Bovinos , Contaminação de Alimentos/análise , Formaldeído/química , Haptenos/química , Haptenos/metabolismo , Humanos , Lactonas/química , Lactonas/metabolismo , Masculino , Coelhos , Medição de Risco , Sensibilidade e Especificidade , Soroalbumina Bovina/química , Soroalbumina Bovina/metabolismo , Zea mays/químicaRESUMO
The use of an indirect competitive enzyme-linked immune sorbent assay (ELISA) involving polyclonal rabbit antibodies against BSA-conjugated PR-toxin (sensitivity, 1 ng/ml) established the ability to synthesize PR-toxin in 18 out of 35 morphologically identified strains of Penicillium roqueforti and P. chrysogenum. The results indicate that ELISA for PR-toxin may be used in assessing the taxonomical position of terverticillate penicillia in the presence of other micotoxins.