Radiographic assessment of splenic size and correlation with splenic measurements estimated by use of computed tomography in healthy cats.

OBJECTIVE: To investigate radiographic variables for correlation with splenic size as estimated with CT in cats. ANIMALS: 38 healthy adult cats. PROCEDURES: The width and height of the splenic head and total length, segmental length, and width of the spleen were measured on radiographic and CT images obtained from 10 cats in prospective, exploratory experiments. Distance between the splenic head and left kidney, anatomic locations of the head and tail of the spleen, and CT-derived splenic volume were also assessed. Correlation and agreement between radiographic and CT measurements and interobserver agreement for measurements with each method were determined. A retrospective evaluation of radiographs obtained without sedation or anesthesia for 28 cats was performed to establish preliminary guidelines for the measurement deemed the most reliable estimator of splenic size. RESULTS: Radiographic measurements of total and segmental splenic length were significantly correlated with the respective CT measurements and with splenic volume. Agreement between radiographic and CT measurements of segmental length was good; interobserver agreement was excellent for all variables. In retrospective evaluations, median segmental length of the spleen was 57.87 mm (range, 34.72 to 105.44 mm) on radiographs; the caudal border of the splenic head on lateral views was located from the cranial part of L1 to the caudal part of L2, and the caudal border of the splenic tail on ventrodorsal views was located from the caudal part of L2 to the caudal part of L5. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated that segmental length of the spleen on radiographs is a reliable estimator of splenic size in healthy cats.

Assessment of neovascularization during bone healing using contrast-enhanced ultrasonography in a canine tibial osteotomy model: a preliminary study.

Blood perfusion of skeletal muscle and callus was evaluated using contrast-enhanced ultrasonography (CEUS) in a canine osteotomy model to determine the applicability of CEUS in the assessment of neovascularization during fracture healing and to compare the vascular signals on CEUS between external skeletal fixation and cast-applied dogs. In 6 Beagle dogs, a simple transverse osteotomy was performed at the left tibial shaft and external skeletal fixation (n = 3) or a cast (n = 3) was applied. Radiography, power Doppler ultrasonography (power Doppler), and CEUS were performed until complete union was achieved. On CEUS, vascular changes were quantitatively evaluated by measuring peak intensity (PI) and time to PI in the soft tissue and callus and by counting the vascular signals. Vascular signals from the soft tissue were detected on power Doppler and CEUS on day 2. Significantly more vascular signals were detected by CEUS than by power Doppler. On CEUS, PI in the surrounding soft tissue was markedly increased after the fracture line appeared indistinctively changed on radiography in all dogs. In the cast-applied dogs, vascular signals from the periosteal and endosteal callus were detected on CEUS before mineralized callus was observed on radiography. CEUS was useful in assessing the vascularity of soft tissue and callus, particularly in indirect fracture healing, and provided indications of a normally healing fracture.

Assessment of a split-bolus computed tomographic enterography technique for simultaneous evaluation of the intestinal wall and mesenteric vasculature of dogs.

OBJECTIVE: To investigate the diagnostic usefulness of split-bolus CT enterography in dogs. ANIMALS: 6 healthy Beagles. PROCEDURES: CT enterography was performed in all dogs in a nonrandomized crossover study design involving 3 techniques: a dual-phase technique and 2 techniques involving splitting of the administered contrast agent dose (ie, split technique and split-bolus tracking technique). For the 2 techniques involving dose splitting (ie, split CT enterography), contrast agent was injected twice, with the first injection consisting of 60% of the total dose, followed by injection of the remaining 40%. Then, a single set of CT images was obtained when the arterial and venous phases matched (dual-phase and split techniques) or when enhancement of the abdominal aorta reached 100 HU (split-bolus tracking technique). Enhancement of the intestinal wall and mesenteric vessels was assessed qualitatively and quantitatively. RESULTS: The total number of images required for interpretation was significantly lower for the split technique than for the dual-phase technique. The amount of time needed to complete CT enterography was significantly less for the split-bolus tracking technique than for the other 2 techniques. For all 3 techniques, adequate contrast enhancement of the mesenteric vessels and intestinal wall was achieved. The split technique provided contrast enhancement of the intestinal wall and mesenteric vessels similar to that provided with the dual-phase technique, whereas contrast enhancement of these structures was lowest for the split-bolus tracking technique. CONCLUSIONS AND CLINICAL RELEVANCE: Split-bolus CT enterography at a contrast agent allocation ratio of 60:40 enabled simultaneous evaluation of the enhanced intestine wall and mesenteric vessels and yielded image quality similar to that of dual-phase CT enterography in healthy dogs.

Comparison of elastography, contrast-enhanced ultrasonography, and computed tomography for assessment of lesion margin after radiofrequency ablation in livers of healthy dogs.

OBJECTIVE To assess by use of various diagnostic imaging modalities acute changes in livers of healthy dogs after radiofrequency ablation (RFA) and determine the capability of each imaging modality to monitor ablation lesion changes. ANIMALS 6 healthy Beagles. PROCEDURES 12 ablation lesions were created in the liver of the dogs (2 lesions/dog). Ablation lesions were evaluated by use of conventional ultrasonography, strain elastography, and contrast-enhanced ultrasonography immediately after (time 0), 30 to 60 minutes after, and 3 days after RFA, and by use of CT 30 minutes and 3 days after RFA. Three dogs were euthanized shortly after RFA, and the other 3 dogs were euthanized on day 3. Lesion size measured by each imaging modality was compared with necropsy findings. RESULTS Immediately after RFA, clear margins were more visible with elastography and contrast-enhanced ultrasonography than with conventional ultrasonography, which had acoustic shadowing. On triphasic contrast CT, the ablation zone, which indicated necrosis and hemorrhage, was not enhanced and could be measured. Marked enhancement of the periablation rim was observed during the venous phase and was identified as granulation tissue. Size of the ablation area measured on enhanced CT images was strongly correlated with actual lesion size. CONCLUSIONS AND CLINICAL RELEVANCE For dogs of this study, CT was the most reliable method for lesion size determination. Although ultrasonographic imaging measurements underestimated lesion size, all modalities could be used to provide additional real-time guidance for RFA procedures of the liver as well as for other RFA procedures.