Characteristics, sources, and health risk assessment of atmospheric particulate mercury in Guanzhong Basin.

Mercury (Hg) has received increasing public attention owing to its high toxicity and global distribution capability via long-range atmospheric transportation. Guanzhong Basin (GB) is vital for the industrial and economic development of Shaanxi Province. To determine the concentration, spatial distribution, seasonal variation, sources, and health risks of particulate-bound mercury (PBM), PM2.5 samples were collected at three sampling sites representing urban, rural, and remote areas during winter and summer in GB. The three sampling sites were in Xi'an (XN), Taibai (TB), and the Qinling Mountains (QL). The mean PBM concentrations in XN, TB, and QL in winter were 130 ± 115 pg m-3, 57.5 ± 47.3 pg m-3, and 53.6 ± 38.5 pg m-3, respectively, higher than in summer (13.7 ± 7.11 pg m-3, 8.01 ± 2.86 pg m-3, and 7.75 ± 2.85 pg m-3, respectively). PBM concentrations are affected by precipitation, meteorological conditions (temperature and mixed boundary layer), emission sources, and atmospheric transport. During the sampling period, the PBM dry deposition in XN, TB, and QL was 1.90 µg m-2 (2 months), 0.835 µg m-2 (2 months), and 0.787 µg m-2 (2 months), respectively, lower than the range reported in national megacities. According to backward trajectory and potential source contribution factor (PSCF) analysis, mercury pollution in XN is mainly affected by local pollution source emissions, whereas the polluted air mass in TB and QL originates from local anthropogenic emissions and long-distance atmospheric transmission. The non-carcinogenic health risk values of PBM in XN, TB, and QL in winter and summer were less than 1, indicating that the risk of atmospheric PBM to the health of the residents was negligible.

Assessment of tolerance limits of petroleum residues in soil organic matter: sorption of dichlorobenzene by soil.

Soil organic matter can protect plants and microorganisms from toxic substances. Beyond the tolerance limit, the toxicity of petroleum pollution to soil organisms may increase rapidly with the increase of petroleum content. However, the method for evaluating the petroleum tolerance limit of soil organic matter (SOM) is still lacking. In this study, the petroleum saturation limit in SOM was first evaluated by the sorption coefficient (Kd) of 1,2-dichlorobenzene (DCB) from water to soils containing different petroleum levels. The sorption isotherm of dichlorobenzene in several petroleum-contaminated soils with different organic matter content and the microbial toxicity test of several petroleum-contaminated soils were determined. It is found that when the petroleum content is about 5% of the soil organic matter content, the sorption of petroleum to organic matter reached saturation limit. When organic matter reaches petroleum saturation limit, the sorption coefficient of DCB by soil particles increased linearly with the increase of petroleum content (R2 > 0.991). The results provided important insights into the understanding the fate of petroleum pollutants in soil and the analysis of soil toxicity.

The change of healthcare service in Chinese patients with inflammatory bowel disease during the pandemic: a national multicenter cross-sectional study.

The pandemic of COVID-19 was a major public health events and had a deeply impact on the healthcare acquired by patients with inflammatory bowel disease (IBD). The purpose of this study was to evaluate the long-term impacts on healthcare service in Chinese IBD patients under the dynamic zero-COVID strategy. The study was performed in the Inflammatory Bowel Disease Quality of Care Centers in mainland China in 2021. The data about the healthcare was collected by a 44-item questionnaire. Totally 463 were from ulcerative colitis (UC) patients and 538 from Crohn's disease (CD) patients were included in the study. The pandemic impacted 37.5% patients on their treatment, and the biggest problem was unable to follow up timely (77.9%). There was a significant increase in healthcare costs in CD (P < 0.001) and no significant change in UC (P = 0.14) after the outbreak. Both UC and CD had an increase in the frequency of outpatient visits (UC 5.07 vs. 4.54, P = 0.001; CD 6.30 vs. 5.76, P = 0.002), and hospitalizations (UC 1.30 vs. 1.02, P < 0.001; CD 3.55 vs. 2.78, P < 0.001). The hospitalization rate in UC reduced slightly (40.2% vs. 42.8%, P = 0.423) after the outbreak, but it significantly increased in CD (75.8% vs. 67.8%, P = 0.004). The rate of biologics had significant increased (UC 11.2% vs. 17.7%, P = 0.005; CD 53.2% vs. 71.0%, P < 0.001). Besides, the proportion of people using telemedicine also increased from 41.6% to 55.1% (P < 0.001). However, 82.8% patients still preferred face-to-face visits. Recurrent outbreaks and the regular pandemic prevention and control policy had a long-term impact on medical care service for IBD patients. The preferred mode of healthcare was still face-to-face visit. It will be a long way to go in the construction of telemedicine in China.

Global, regional, and national burdens of atrial fibrillation/flutter from 1990 to 2019: An age-period-cohort analysis using the Global Burden of Disease 2019 study.

Background: Atrial fibrillation/flutter (AF/AFL) significantly impacts countries with varying income levels. We aimed to present worldwide estimates of its burden from 1990 to 2019 using data from the Global Burden of Disease (GBD) study. Methods: We derived cause-specific AF/AFL mortality and disability-adjusted life-year (DALY) estimates from the GBD 2019 study data. We used an age-period-cohort (APC) model to predict annual changes in mortality (net drifts), annual percentage changes from 50-55 to 90-95 years (local drifts), and period and cohort relative risks (period and cohort effects) between 1990 and 2019 by sex and sociodemographic index (SDI) quintiles. This allowed us to determine the impacts of age, period, and cohort on mortality and DALY trends and the inequities and treatment gaps in AF/AFL management. Results: Based on GBD data, our estimates showed that 59.7 million cases of AF/AFL occurred worldwide in 2019, while the number of AF/AFL deaths rose from 117 000 to 315 000 (61.5% women). All-age mortality and DALYs increased considerably from 1990 to 2019, and there was an increase in age risk and a shift in death and DALYs toward the older (>80) population. Although the global net drift mortality of AF/AFL decreased overall (-0.16%; 95% confidence interval (CI) = -0.20, 0.12 per year), we observed an opposite trend in the low-middle SDI (0.53%; 95% CI = 0.44, 0.63) and low SDI regions (0.32%; 95% CI = 0.18, 0.45). Compared with net drift among men (-0.08%; 95% CI = -0.14, -0.02), women had a greater downward trend or smaller upward trend of AF/AFL (-0.21%; 95% CI = -0.26, -0.16) in mortality in middle- and low-middle-SDI countries (P < 0.001). Uzbekistan had the largest net drift of mortality (4.21%; 95% CI = 3.51, 4.9) and DALYs (2.16%; 95% CI = 2.05, 2.27) among all countries. High body mass index, high blood pressure, smoking, and alcohol consumption were more prevalent in developed countries; nevertheless, lead exposure was more prominent in developing countries and regions. Conclusions: The burden of AF/AFL in 2019 and its temporal evolution from 1990 to 2019 differed significantly across SDI quintiles, sexes, geographic locations, and countries, necessitating the prioritisation of health policies based on risk-differentiated, cost-effective AF/AFL management.

Improved neural network for predicting blood donations based on two emergent factors.

BACKGROUND: Blood donation forecasting is a critical part of blood supply chain management. However, few studies have focused on modeling blood donation with different emergency factors. The purpose of this study was to investigate the effects of different emergency events on blood donation and to build a suitable blood volume prediction model. MATERIALS AND METHODS: The amount of blood donation from 2015 to December 2021 at Beijing Tongzhou District Central Blood Station was selected as the time series data. First, statistical methods were employed to analyze the effect of weather and epidemic factors on blood donation. Second, a hybrid model of SARIMAX and a neural network was built to predict the blood donation in the next two weeks with two factors. RESULTS: We identified significant differences in blood donations under different emergency conditions and a high correlation between epidemic status and blood donations. In addition, the decision coefficient improved by 60.7%, and the Root Mean Square Error(RMSE) decreased by 1.668 when using the hybrid model of SARIMAX and the neural network, indicating that the model was effective in reducing the prediction error of blood donation. CONCLUSION: The hybrid model approach allows long-term forecasting of blood donations under emergency conditions and provides reliable and accurate forecasting results for blood stations up to 2 weeks in advance, facilitating warnings on the blood supply to relevant hospitals and improving hospital treatment rates while reducing blood transportation costs.

Sequential damage assessment of the posterolateral complex of the knee joint: a finite element study.

BACKGROUND: The posterolateral complex (PLC), which consists of the popliteus tendon (PT), lateral collateral ligament (LCL), and popliteofibular ligament (PFL), is an indispensable structure of the knee joint. The aim of this study was to explore the functionality of the PLC by determining the specific role of each component in maintaining posterolateral knee stability. METHODS: A finite element (FE) model was generated based on previous material property data and magnetic resonance imaging of a volunteer's knee joint. The injury order of the PLC was set as LCL, PFL, and PT. A combined compressive load of 1150 N and an anterior tibial load of 134 N was applied to the tibia to investigate tibial displacement (TD). Tibial external rotation (TER) and tibial varus angulation (TVA) were measured under bending motions of 5 and 10 Nm. The instantaneous axis of rotation (IAR) of the knee joint under different rotation motions was also recorded. RESULTS: The TD of the intact knee under a combined compressive load of 1150 N and an anterior tibial load of 134 N matched the values determined in previous studies. Our model showed consistent increases in TD, TVA, and TER after sequential damage of the PLC. In addition, sequential disruption caused the IAR to shift superiorly and laterally during varus rotation and medially and anteriorly during external rotation. In the dynamic damage of the PLC, LCL injury had the largest effect on TD, TVA, TER, and IAR. CONCLUSIONS: Sequential injury of the PLC caused considerable loss of stability of the knee joint according to an FE model. The most significant structure of the PLC was the LCL.

Immobilization of Cr(VI) in Soil Using a Montmorillonite-Supported Carboxymethyl Cellulose-Stabilized Iron Sulfide Composite: Effectiveness and Biotoxicity Assessment.

A novel composite of montmorillonite-supported carboxymethyl cellulose-stabilized nanoscale iron sulfide (CMC@MMT-FeS), prepared using the co-precipitation method, was applied to remediate hexavalent chromium (Cr(VI))-contaminated soil. Cr(VI)-removal capacity increased with increasing FeS-particle loading. We tested the efficacy of CMC@MMT-FeS at three concentrations of FeS: 0.2, 0.5, and 1 mmol/g, hereafter referred to as 0.2 CMC@MMT-FeS, 0.5 CMC@MMT-FeS, and 1.0 CMC@MMT-FeS, respectively. The soil Cr(VI) concentration decreased by 90.7% (from an initial concentration of 424.6 mg/kg to 39.4 mg/kg) after 30 days, following addition of 5% (composite-soil mass proportion) 1.0 CMC@MMT-FeS. When 2% 0.5 CMC@MMT-FeS was added to Cr(VI)-contaminated soil, the Cr(VI) removal efficiency, as measured in the leaching solution using the toxicity characteristic leaching procedure, was 90.3%, meeting the environmental protection standard for hazardous waste (5 mg/kg). The European Community Bureau of Reference (BCR) test confirmed that the main Cr fractions in the soil samples changed from acid-exchangeable fractions to oxidable fractions and residual fractions after 30 days of soil remediation by the composite. Moreover, the main complex formed during remediation was Fe(III)-Cr(III), based on BCR and X-ray photoelectron spectroscopy analyses. Biotoxicity of the remediated soils, using Vicia faba and Eisenia foetida, was analyzed and evaluated. Our results indicate that CMC@MMT-FeS effectively immobilizes Cr(VI), with widespread potential application in Cr(VI)-contaminated soil remediation.

Safety and effectiveness of the da Vinci robot with the "3+2" mode for distal pancreatectomy.

BACKGROUND: Recently, no relevant research has focused on the relationship between the clinical efficacy of da Vinci robotic distal pancreatectomy (RDP) and the number of mechanical arms and assistants used for RDP. The aim of this study was to evaluate the safety, efficacy, and advantages of RDP with the "3 + 2" mode. METHODS: Clinical data from 53 patients (observation group) who received RDP using the "3 + 2" mode in our department, from March 2016 to September 2018, were reviewed. An additional 53 patients who received RDP using the classical mode were chosen at random for the control group. Short-term outcomes for the two groups were compared. RESULTS: There were no statistically significant differences between the two groups for estimated blood loss, postoperative day of flatus passage, postoperative hospital stay, and postoperative complication (P > 0.05). Compared with the control group, the observation group had a significantly shorter operative time (166.9 ± 13.3 vs 192.6 ± 11.1 minutes, P < 0.001), lower surgical costs ($2827.79 ± $173.02 vs $3900.63 ± $317.29, P < 0.001). CONCLUSIONS: The RDP using the "3 + 2" mode can increase the exposure of surgical field, improve cooperation between assistants, lower the surgical costs, and shorten the operative time and learning curve. Moreover, the clinical effect is equal to that of RDP using the classical mode. These findings indicate that RDP using the "3 + 2" mode is safe and feasible for institutions that are equipped for robot-assisted surgery.

Effectiveness and safety of robotic-assisted versus laparoscopic hepatectomy for liver neoplasms: A meta-analysis of retrospective studies.

This meta-analysis aimed to investigate the effectiveness and safety of RAH and LLR for liver neoplasms. A systematic search was performed in PubMed, EMbase, the Cochrane Library, Web of science, and China Biology Medicine disc up to July 2016 for studies that provided comparisons between the surgical outcomes of RAH and LLR for liver neoplasms. WMD, OR and 95% CI were calculated and data combined using the random-effect model. The quality of the evidence was assessed using GRADE methods. A total of 17 studies were included in the meta-analysis, in which 487 patients were in the RAH group and 902 patients were in the LLR group. The meta-analysis results indicated: compared to LLR, RAH was associated with more estimated blood loss, longer operative time, and longer time to first nutritional intake (p < 0.05). There was no significant difference in length of hospital stay, conversion rate during operation, R0 resection rate, complications and mortality (p > 0.05). Three studies reported the total cost, and the result showed a higher cost in the RAH group when compared with the LLR group (p < 0.05). This meta-analysis indicated that RAH and LLR display similar effectiveness and safety in hepatectomy. Considering the lack of high quality original studies, prospective clinical trials should be conducted to provide strong evidence for clinical guidelines formation, and the insurance coverage policies should be established to promote the application of robotic surgery in the future.

Assessment of epidermal growth factor receptor mutation/copy number and K-ras mutation in esophageal cancer.

BACKGROUND: The molecular status of epidermal growth factor receptor (EGFR) in esophageal cancer has not been well elucidated. The purpose of the study was to investigate the prevalence of EGFR and K-ras mutation, and EGFR gene copy number status as well as its association with clinicopathologic characteristics, and also to identify the prognostic value of EGFR gene copy number in esophageal cancer. METHODS: EGFR mutation in exon 19/exon 21 and K-ras mutation in codon 12/codon 13 were detected by real-time PCR method, while EGFR gene copy number status was analyzed by fluorescent in situ hybridization (FISH). EGFR gene amplification and high polysomy were defined as high EGFR gene copy number status (FISH-positive), and all else were defined as low EGFR gene copy number status (FISH-negative). The relationship between EGFR gene copy number status and clinicpathologic characteristics was analyzed. Kaplan-Meier method and Cox proportional hazards regression model were employed to evaluate the effects of EGFR gene copy number status on the patients' survival. RESULTS: A total of 57 esophageal squamous cell carcinoma (ESCC) patients and 9 esophageal adenocarcinoma (EADC) patients were enrolled in the study. EGFR mutation was identified in one patient who was diagnosed as ESCC with stage IIIC disease. K-ras mutation was identified in one patient who was diagnosed as EADC. In all, 34 of 66 (51.5%) samples were detected as FISH-positive, which includes 30 ESCC and 4 EADC tumor samples. The correlation analysis showed that FISH-positive was significantly associated with the tumor stage (P=0.019) and lymph node metastasis (P=0.005) in esophageal cancer patients, and FISH-positive was also significantly associated with the tumor stage (P=0.007) and lymph node metastasis (P=0.008) in ESCC patients. Cox regression analysis showed that high EGFR gene copy number was not a significant predictor of a poor outcome for esophageal cancer patients (P=0.251) or for ESCC patients (P=0.092), but esophageal cancer patients or ESCC patients with low EGFR gene copy number may have longer survival than those with high EGFR gene copy number according to the survival curve trends. CONCLUSIONS: The results indicated that EGFR or K-ras mutation was rare in esophageal cancer, but high EGFR gene copy number is frequent, and correlated with advanced pathologic stage and more number of the metastatic regional lymph nodes, especially in ESCC. In addition, high EGFR gene copy number is likely to have a deleterious effect on prognosis of esophageal cancer patients or ESCC patients, although no statistical significance was reached in the study.

Investigation into atmospheric PM2.5-borne PAHs in Eastern cities of China: concentration, source diagnosis and health risk assessment.

This study investigated PM2.5-PAHs associations collected in Beijing, Jinan, and Shanghai in Eastern China. The results indicated that PM2.5 concentrations in Beijing, Jinan, and Shanghai were 125.7 µg m(-3) (18.6-355.5 µg m(-3)), 115.9 µg m(-3) (44.2-345.4 µg m(-3)), and 85.1 µg m(-3) (24.3-232.8 µg m(-3)), respectively. The PAH concentrations in terms of PM2.5 in Beijing, Jinan, and Shanghai ranged from 23.2 to 819.8 ng m(-3), 25.7 to 727.1 ng m(-3), and 8.5 to 133.9 ng m(-3), respectively. PAH concentrations were found to be positively correlated with PM2.5 concentration in Beijing and Shanghai. The compositions of PAHs in PM2.5 in Beijing and Jinan were almost the same: 11% low ring, 80-82% middle ring, and 7-9% high ring. However, Shanghai had a different composition. Source apportionment indicated that the incomplete combustion of coal and diesel and gasoline emissions were the main sources of PAHs in PM2.5 in all three cities, whereas Shanghai had a greater contribution from liquid fossil fuels. The values for the health risk assessment estimated by the benzo[a]pyrene equivalent concentration in Beijing and Jinan were 2.39 × 10(-6) and 2.57 × 10(-6), respectively, thus both exceeding the 1 × 10(-6) limit (USEPA) considered likely to pose an inhalation cancer risk to people. Shanghai, however, had a risk estimate of 5.05 × 10(-7), which is still in a safe range. This study is the first to simultaneously monitor the PAHs in PM2.5 in three cities in Eastern China and may point to a long-range transportation of PM2.5-PAHs from Beijing to Jinan and partially to Shanghai.

Driving risk assessment using near-crash database through data mining of tree-based model.

This paper considers a comprehensive naturalistic driving experiment to collect driving data under potential threats on actual Chinese roads. Using acquired real-world naturalistic driving data, a near-crash database is built, which contains vehicle status, potential crash objects, driving environment and road types, weather condition, and driver information and actions. The aims of this study are summarized into two aspects: (1) to cluster different driving-risk levels involved in near-crashes, and (2) to unveil the factors that greatly influence the driving-risk level. A novel method to quantify the driving-risk level of a near-crash scenario is proposed by clustering the braking process characteristics, namely maximum deceleration, average deceleration, and percentage reduction in vehicle kinetic energy. A classification and regression tree (CART) is employed to unveil the relationship among driving risk, driver/vehicle characteristics, and road environment. The results indicate that the velocity when braking, triggering factors, potential object type, and potential crash type exerted the greatest influence on the driving-risk levels in near-crashes.

Development of a general method for detection and quantification of the P35S promoter based on assessment of existing methods.

The Cauliflower mosaic virus (CaMV) 35S promoter (P35S) is a commonly used target for detection of genetically modified organisms (GMOs). There are currently 24 reported detection methods, targeting different regions of the P35S promoter. Initial assessment revealed that due to the absence of primer binding sites in the P35S sequence, 19 of the 24 reported methods failed to detect P35S in MON88913 cotton, and the other two methods could only be applied to certain GMOs. The rest three reported methods were not suitable for measurement of P35S in some testing events, because SNPs in binding sites of the primer/probe would result in abnormal amplification plots and poor linear regression parameters. In this study, we discovered a conserved region in the P35S sequence through sequencing of P35S promoters from multiple transgenic events, and developed new qualitative and quantitative detection systems targeting this conserved region. The qualitative PCR could detect the P35S promoter in 23 unique GMO events with high specificity and sensitivity. The quantitative method was suitable for measurement of P35S promoter, exhibiting good agreement between the amount of template and Ct values for each testing event. This study provides a general P35S screening method, with greater coverage than existing methods.

A human health risk assessment of rare earth elements in soil and vegetables from a mining area in Fujian Province, Southeast China.

Contaminated food through dietary intake has become the main potential risk impacts on human health. This study investigated concentrations of rare earth elements (REEs) in soil, vegetables, human hair and blood, and assessed human health risk through vegetables consumption in the vicinity of a large-scale mining area located in Hetian Town of Changting County, Fujian Province, Southeast China. The results of the study included the following mean concentrations for total and bio-available REEs of 242.92 ± 68.98 (135.85-327.56)µg g(-1) and 118.59 ± 38.49 (57.89-158.96)µg g(-1) dry weight (dw) in agricultural soil, respectively, and total REEs of 3.58 ± 5.28 (0.07-64.42)µg g(-1) dw in vegetable samples. Concentrations of total REEs in blood and hair collected from the local residents ranged from 424.76 to 1274.80 µg L(-1) with an average of 689.74 ± 254.25 µg L(-1) and from 0.06 to 1.59 µg g(-1) with an average of 0.48 ± 0.59 µg g(-1) of the study, respectively. In addition, a significant correlation was observed between REEs in blood and corresponding soil samples (R(2)=0.6556, p<0.05), however there was no correlation between REEs in hair and corresponding soils (p>0.05). Mean concentrations of REEs of 2.85 (0.59-10.24)µg L(-1) in well water from the local households was 53-fold than that in the drinking water of Fuzhou city (0.054 µg L(-1)). The health risk assessment indicated that vegetable consumption would not result in exceeding the safe values of estimate daily intake (EDI) REEs (100-110 µg kg(-1)d(-1)) for adults and children, but attention should be paid to monitoring human beings health in such rare earth mining areas due to long-term exposure to high dose REEs from food consumptions.

Assessment of brain maturation in the preterm infants using diffusion tensor imaging (DTI) and enhanced T2 star weighted angiography (ESWAN).

PURPOSE: To assess the brain maturation of preterm infants using diffusion tensor imaging (DTI) and enhanced T2 star weighted angiography (ESWAN). MATERIALS AND METHODS: Conventional magnetic resonance imaging (MRI), DTI and ESWAN were performed in 60 preterm infants and 21 term controls. 60 preterm infants were subgrouped to two groups according to the age at imaging: before and at term-equivalent age (TEA). Fractional anisotropy (FA), apparent diffusion coefficient (ADC) map from DTI, T2 and R2 maps from ESWAN were post-processed at an off-line workstation. The values of FA, ADC, T2 and R2 from the posterior limb of internal capsule (PLIC), frontal white matter (FWM), occipital white matter (OWM) and lentiform nuclei (LN) were determined. These parameters were compared between preterm and term infants. Correlations of DTI and ESWAN parameters with the gestational age, postmenstrual age and postnatal age were analyzed. RESULTS: ADCs of FWM, OWM and LN, and T2 values of the PLIC and LN were higher in the preterm infants at TEA compared with the term controls. The correlations were existed between the postmenstrual age and the values of FA, ADC, T2, R2 from the PLIC, values of ADC, T2, R2 from the LN, T2 value from the OWM. The correlations were also found between the postnatal age and the values of FA, ADC, T2 from the PLIC, and T2 value from the LN. CONCLUSION: The maturity of preterm brain around TEA was different from that of term controls and appeared to be independent of the prematurity at birth. T2 was one of valuable indices to evaluate brain maturation in preterm infants.