Plant Flavonoids with Antimicrobial Activity against Methicillin-Resistant Staphylococcus aureus (MRSA).

Methicillin-resistant Staphylococcus aureus (MRSA) has become a serious threat to human public health and global economic development, and there is an urgent need to develop new antimicrobial agents. Flavonoids are the largest group of plant secondary metabolites, and the anti-S. aureus and anti-MRSA activities of flavonoids have now been widely reported. The aim of this Review is to describe plant-derived flavonoid active ingredients and their effects and mechanisms of inhibitory activity against MRSA in order to provide insights for screening novel antimicrobial agents. Here, 85 plant-derived flavonoids (14 flavones, 21 flavonols, 26 flavanones, 9 isoflavones, 12 chalcones, and 3 other classes) with anti-MRSA activity are reviewed. Among these flavonoids, flavones and isoflavones generally showed the most significant anti-MRSA activity (MICs: 1-8 µg/mL). The results of the present Review display that most of the flavonoids with excellent anti-MRSA activity were derived from Morus alba L. and Paulownia tomentosa (Thunb.) Steud. The antibacterial mechanism of flavonoids against MRSA is mainly achieved by disruption of membrane structures, inhibition of efflux pumps, and inhibition of ß-lactamases and bacterial virulence factors. We hope this Review can provide insights into the development of novel antimicrobials based on natural products for treating MRSA infections.

Vascularized fascial flap for reconstruction of combined oral mucosa-mandibular defects: The multi-modal biological assessment.

OBJECTIVE: The reconstruction of composite defects in the oral and maxillofacial region using vascularized fascial flaps, such as the fibular, iliac, and temporal fascial flaps, has gained increasing attention among surgeons. However, there remains uncertainty regarding the suitability of fascial flaps as transplants, as well as their healing processes and outcomes, due to their non-mucosal nature. This study aims to comprehensively assess the biological aspects of vascularized fascial flaps at clinical, histological, and genetic levels, with the goal of providing essential biological references for their clinical application. STUDY DESIGN: This study enrolled three patients who underwent reconstruction of combined oral mucosa-mandibular defects using fibular vascularized fascial flaps between 2020 and 2023. Data regarding changes in the appearance of the fascial flaps, bulk-RNA sequencing, and histological slices of initial fascia, initial gingiva, and transformed fascia were collected and analyzed. RESULTS: Within three months, the fascial flaps exhibited rapid epithelial coverage and displayed distinct characteristics resembling mucosa. High-throughput RNA sequencing analyses and histological slices revealed that the transformed fascia exhibited tissue structures similar to mucosa and demonstrated unique advantages in promoting blood vessel formation and reducing scarring through the high-level expression of relevant genes. CONCLUSION: These findings emphasize the potential and feasibility of utilizing vascularized fascial flaps for oral mucosa reconstruction, establishing their unique advantage as transplant materials, and providing significant biological information and references for their selection and clinical application.

Safety assessment of organic micropollutants in reclaimed water: Chemical analyses, ecological risk assessments, and in vivo endocrine-disrupting studies.

Reclaimed water from municipal wastewater has great potential in mitigating the water resource crisis, while the inevitable residue of organic micropollutants (OMPs) challenges the safety of reclaimed water reuse. Limited information was available regarding the overall adverse effects of mixed OMPs in reclaimed water, especially the endocrine-disrupting effects on living organisms. Herein, chemical monitoring in two municipal wastewater treatment plants showed that 31 of 32 candidate OMPs including polycyclic aromatic hydrocarbons (PAHs), phenols, pharmaceuticals and personal care products (PPCPs) were detected in reclaimed water, with a concentration ranging from ng/L to µg/L. Then, based on the risk quotient value, phenol, bisphenol A, tetracycline, and carbamazepine were ranked as high ecological risks. Most PAHs and PPCPs were quantified as medium and low risks, respectively. More importantly, using aquatic vertebrate zebrafish as an in vivo model, the endocrine-disrupting potentials of OMP mixtures were comprehensively characterized. We found that a realistic exposure to reclaimed water induced estrogen-like endocrine disruption and hyperthyroidism in zebrafish, abnormal expression of genes along the hypothalamus-pituitary-thyroid (-gonad) axes, reproductive impairment, and transgenerational toxicity. Based on the chemical analyses, risk quotient calculations, and biotoxicity characterization, this study contributed to understanding the ecological risks of reclaimed water and developing the control standards for OMPs. In addition, application of the zebrafish model in this study also highlighted the significance of in vivo biotoxicity test in water quality evaluation.

Risk assessment and validation of farmland abandonment based on time series change detection.

Farmland abandonment, a widespread phenomenon during land-use transition, leads to a cycling or vanishing evolution of farmland resources. As urbanization advances, an increasing number of agricultural laborers migrate from rural to urban areas, causing ongoing farmland abandonment. However, in contrast to the abandoned information extraction and driving mechanisms revelation, the potential risk of farmland abandonment has received insufficient attention. This study took Yangtze River Economic Belt of China as study area, selected multiple aspects to construct a risk assessment system for farmland abandonment, and applied time series change detection to verify the results. The results showed that (1) farmland abandonment risk, with a regional average value of 0.0978, has strong spatial heterogeneity, with high values clustering in Yunnan-Guizhou and Sichuan-Chongqing mountainous areas and low values distributed in the midstream and downstream plains and the Sichuan Basin. (2) The proportion of farmland area gradually decreased as the risk grade increased. Farmland, with low abandonment risk, occupied an area of 204,837 km2, constituting the highest percentage of 35.18% among the overall farmland, and was mainly distributed in the provinces of Jiangsu and Anhui. The area of farmland with high risk was 16,458 km2, only accounting for 2.83%, the majority of which was clustered in Sichuan and Yunnan provinces. (3) The Normalized Difference Vegetation Index (NDVI) time series change detection validated the reliability of the risk assessment system. Samples of farmland having low abandonment risk indeed had the lowest abandonment rate of 10%, and those which indicated high risk had the highest abandonment rate of 32%. We propose differentiated managements for farmland resources with high and low abandonment risk from the perspective of sustainable use. This study provides a more reasonable and scientific system for farmland abandonment risk assessment and helps to fill the research gap.

Influence of family resources on secondhand smoking in pregnant women: a cross-sectional study in the border and minority urban areas of Northwest China.

BACKGROUND: Pregnant women's exposure to secondhand smoke is a very serious health issue in China. The purpose of our research is to identify factors that predict the probability of exposure to secondhand smoke among pregnant women from the perspective of a family-based open system. METHODS: From September 2014 to August 2015, Urumqi City, Shihezi City, and Shawan County-level City were sampled according to population characteristics. A revised structured questionnaire based on family resources was adapted for use in this study. Questionnaires were collected via convenience sampling at the hospitals with the largest number of local antenatal clients. A total of 1249 pregnant women of age 18-51 years were investigated. Descriptive statistics were calculated to characterize the participants and study variables. Binary logistic regression was performed to assess the impact of family resources corresponding variables on the likelihood that participants would be exposed to SHS. Both unadjusted and adjusted odds ratios (OR/AOR) [with 95% confidence intervals (CI)] were reported. RESULTS: The secondhand smoke exposure rate found in this study was 54.6%. Having good knowledge of the dangers of secondhand smoke had no effect on reducing the prevalence of exposure (P > 0.05). Even pregnant women whose husbands who did not use tobacco or never smoked nearby had a risk of exposure to secondhand smoke [adjusted odds ratio (AOR) 1.568, 95% CI 1.205-2.041] when the data were adjusted for age, gravidity, gestational weeks, knowledge of the dangers of secondhand smoke, location, and work status. Home smoking bans were confirmed to be an important protective factor (AOR 1.710, 95% CI 1.549-1.918); however, only one-third (33.5%) of participants reported having a smoking ban at home. Religion (mainly Islam), as a special external family resource, was a protective factor that reduced secondhand smoke exposure in pregnant women (AOR 0.399, 95% CI 0.312-0.510). CONCLUSIONS: The effect of family resources on tobacco control should be considered in the development of effective and enduring strategies for indoor smoking bans and smoking cessation.

Assessment of arsenic (As) occurrence in arable soil and its related health risk in China.

Arsenic (As) is a major global environmental pollutant due to its high toxicity on human and animal health. This study collected 427 relevant papers to study As concentrations in Chinese arable soil and evaluate the health risk of exposure to As for humans. Results showed that the average of As concentration was 9.46 mg/kg in Chinese arable soil. Soil As concentrations in Hunan Province and Guangxi Zhuang Autonomous Region posed high carcinogenic and non-cancer risks on human health through diet, Yunnan, Guizhou, Guangdong, and Xinjiang provinces had relative high health risks, while As concentrations in the other provinces posed low health risks on humans. The physical factors controlled the spatial pattern of health risk on a provincial scale, but the As-related human activities introduced high health risk on people, particularly the agricultural activities such as sewage irrigation and fertilizer application should be given more attention due to its large area.

Assessment of cadmium (Cd) concentration in arable soil in China.

Cadmium (Cd) concentration in arable soil has drawn broad public attention due to its direct effect on Cd concentration in food. However, there have been few studies of surveying Cd accumulation on the national scale in China. This paper collected 486 studies of Cd concentrations in Chinese arable soil. The results showed that the average Cd concentration was 0.27 mg/kg, higher than its background value, indicating that Cd had been introduced into arable soil by human activity. The Cd concentrations in areas of mining and smelting, urban areas, and areas irrigated by wastewater were obviously higher than that in remote areas. Spatially, Cd concentrations were lower in the north than those in the south, and many hotspots existed throughout China due to mining and smelting activities. Most Cd in the arable soil were accumulated from external sources in all investigated provinces except Ningxia Hui Autonomous Region.