Spatial and temporal evolution of urban resilience in China and analysis of barriers: Based on a sustainable development perspective.

With the increasing uncertainty of urban security, urban resilience construction with risk awareness and bottom-line thinking has become essential for promoting sustainable urban development. This paper measures China's urban resilience development index (CRDI) based on 284 cities in China (except Tibet) using the entropy method from four dimensions: economic, social, environmental, and infrastructure, and analyzes it by combining coupling coordination degree and barrier factor analysis. We find that: (1) At the national level, CRDI and its sub-dimensions show an increasing trend in time, a decreasing spatial layout from coastal to inland, and a "high-high-low-low" clustering feature in space. (2) At the regional level, the CRDI is from high to low in the east, middle, and west order. The sub-dimensions are from high to low in the order of east, middle, and west for economic, social, and infrastructure resilience and from high to low in the order of east, west, and middle for environmental resilience. (3) To coupling coordination degree, the CRDI index coupling coordination is increasing in time trend but is still on the verge of dissonance. (4) Social resilience is the main obstacle factor. In the indicator layer, human resources, innovation, education, security, living, and environmental protection are the areas where CRDI coordinated development is the key to improvement. Based on the above empirical evidence, this paper proposes countermeasures to optimize urban resilience construction from four perspectives: economic, social, environmental, and infrastructure.

Metabolic and immune costs balance during natural acclimation of corals in fluctuating environments.

Epigenetic modifications based on DNA methylation can rapidly improve the potential of corals to adapt to environmental pressures by increasing their phenotypic plasticity, a factor important for scleractinian corals to adapt to future global warming. However, the extent to which corals develop similar adaptive mechanisms and their specific adaptation processes remain unclear. Here, to reveal the regulatory mechanism by which DNA methylation improves thermal tolerance in Pocillopora damicornis under fluctuating environments, we analyzed genome-wide DNA methylation signatures in P. damicornis and compared the differences in the methylation and transcriptional responses of P. damicornis from fluctuating and stable environments using whole-genome bisulfite sequencing and nanopore-based RNA sequencingtranscriptome sequencing. We discovered low methylation levels in P. damicornis (average methylation 4.14%), with CpG accounting for 74.88%, CHH for 13.27%, and CHG for 11.85% of this methylation. However, methylation levels did not change between coral samples from the fluctuating and stable environments. The varied methylation levels in different regions of the gene revealed that the overall methylation level of the gene body was relatively high and showed a bimodal methylation pattern. Methylation occurs primarily in exons rather than introns within the gene body In P. damicornis, there was only a weak correlation between methylation and transcriptional changes at the individual gene level, and the methylation and gene expression levels generally exhibited a bell-shaped relationship, which we speculate may be due to the specificity of cnidarian species. Correlation analysis between methylation levels and the transcriptome revealed that the highest proportion of the top 20 enriched KEGG pathways was related to immunity. Additionally, P. damicornis collected from a high-temperature pool had a lower metabolic rate than those collected from a low-temperature pool. We hypothesize that the dynamic balance of energy-expenditure costs between immunity and metabolism is an important strategy for increasing P. damicornis tolerance. The fluctuating environment of high-temperature pools may increase the heat tolerance in corals by increasing their immunity and thus lowering their metabolism.

Spatial and temporal evolution of urban economic development efficiency in China's Yangtze River economic belt from the perspective of sustainable development.

Urban economic development is crucial to regional economy and people's life, and enhancing the efficiency of urban economic development is of great significance to boost sustainable and healthy economic and social development. In this paper, from the perspective of sustainable development, data of 104 cities in China's Yangtze River Economic Belt (YREB) from 2004 to 2019 are selected, and the urban resource consumption index and urban pollutant emission index are synthesized as new input-output indicators using the Time Series Global Principal Component Analysis (GPCA), combined with the Global Malmquist-Luenberger (GML) Index Model, Standard Deviation Ellipse (SDE) Model to measure the total factor productivity index of urban economic development in China's YREB and analyze its spatial and temporal evolution. The results show that from 2004 to 2019, the total factor productivity index of urban economic development in China's YREB showed an overall fluctuating upward trend with an average annual growth of 5.8%, and the analysis by decomposing indicators shows that the growth of total factor productivity of urban economic development in China's YREB is mainly influenced by the growth of technological progress. Meanwhile, there are obvious regional differences in the efficiency of urban economic development in China's YREB, with the largest difference in the middle reaches of the Yangtze River, the second largest in the upper reaches, and the smallest in the lower reaches. From 2004 to 2019, the efficiency center of gravity of urban economic development efficiency in the YREB has always been located in the middle reaches of the Yangtze River region. The spatial distribution pattern of urban economic development efficiency in the YREB is dominated by the northeast-southwest direction and tends to be concentrated in the study time period.

Research on coupling coordination and influencing factors between Urban low-carbon economy efficiency and digital finance-Evidence from 100 cities in China's Yangtze River economic belt.

China is a large country with rapid economic expansion and high energy consumption, which implies that the country's overall carbon emissions are enormous. It is vital to increase urban low-carbon economy efficiency (ULEE) to achieve sustainable development of China's urban economy. Digital finance is a significant tool to boost ULEE by providing a convenient and effective funding channel for urban low-carbon economic transformation. Analyzing the coupled and coordinated relationship between ULEE and digital finance is of vital importance for the sustainable development of the urban economy. This paper selects panel data of 100 cities in China's Yangtze River Economic Belt (YEB) in 2011-2019, and analyzes the research methods such as the Global Malmquist-Luenberger index model, coupling coordination degree (CCD) model, standard deviation ellipse model, gray model, and geographic detector by The spatial and temporal distribution, dynamic evolution characteristics and influencing factors of the CCD between ULEE and digital finance are analyzed. The study shows that: (1) the CCD of ULEE and digital finance grows by 3.42% annually, reflecting the increasingly coordinated development of the two systems; (2) The CCD of ULEE and digital finance shows a distribution pattern of gradient increase from the upstream region of Yangtze River to the downstream region, meanwhile, the spatial center of gravity moves mainly in the midstream region; (3) The spatial center of gravity of CCD of ULEE and digital finance is expected to move 22.17 km to the southwest from 2019 to 2040; (4) In terms of influencing factors, the influence of informatization and industrial structure on the CCD increases over time, while the influence of factors such as population development, greening, transportation, and scientific research decreases over time. Finally, this paper proposes policy recommendations for improving the CCD of ULEE and digital finance based on the empirical results.

Simple, fast, and efficient process for producing and purifying trehalulose.

A new property of recombinant trehalose synthase (GTase) from Thermus thermophilus HB-8 (ATCC 27634) was found and described in this study. GTase can act on sucrose and catalyze trehalulose formation without isomaltose, isomaltulose, or isomelezitose, releasing small amounts of glucose and fructose as byproducts. Maximum trehalulose yield (approximately 81%) was obtained at an optimum temperature of 65°C and was independent of substrate concentration. A simple, fast, and efficient method of producing and purifying trehalulose is then described. In the first step, GTase catalyzed trehalulose formation using a 20% sucrose substrate. Miscellaneous sugars were then rapidly removed, while trehalulose was completely preserved by Saccharomyces cerevisiae cells. Finally, the cells were separated by centrifugation, and salt ions were removed by an ion-exchange resin, subsequently obtaining a high-purity trehalulose solution. A trehalulose recovery rate of over 95% was achieved using this process. This method has a simple process, fast separation efficiency, and low investment in production equipment, so greatly to improve production efficiency and reduce production costs.