Risk assessment, fitness cost, cross-resistance, and mechanism of tetraniliprole resistance in the rice stem borer, Chilo suppressalis.

The rice stem borer (RSB), Chilo suppressalis, a notorious rice pest in China, has evolved a high resistance level to commonly used insecticides. Tetraniliprole, a new anthranilic diamide insecticide, effectively controls multiple pests, including RSB. However, the potential resistance risk of RSB to tetraniliprole is still unknown. In this study, the tetraniliprole-selection (Tet-R) strain was obtained through 10 continuous generations of selection with tetraniliprole 30% lethal concentration (LC30 ). The realized heritability (h2 ) of the Tet-R strain was 0.387, indicating that resistance of RSB to tetraniliprole developed rapidly under the continuous selection of tetraniliprole. The Tet-R strain had a high fitness cost (relative fitness = 0.53). We established the susceptibility baseline of RSB to tetraniliprole (lethal concentration at LC50  = 0.727 mg/L) and investigated the resistance level of 6 field populations to tetraniliprole. All tested strains that had resistance to chlorantraniliprole exhibited moderate- to high-level resistance to tetraniliprole (resistance ratio = 27.7-806.8). Detection of ryanodine receptor (RyR) mutations showed that the Y4667C, Y4667D, I4758M, and Y4891F mutations were present in tested RSB field populations. RyR mutations were responsible for the cross-resistance between tetraniliprole and chlorantraniliprole. Further, the clustered regularly interspaced palindromic repeats (CRISPR) / CRISPR-associated protein 9-mediated genome-modified flies were used to study the contribution of RyR mutations to tetraniliprole resistance. The order of contribution of a single RyR mutation to tetraniliprole resistance was Y4667D > G4915E > Y4667C ≈ I4758M > Y4891F. In addition, the I4758M and Y4667C double mutations conferred higher tetraniliprole resistance than single Y4667C mutations. These results can guide resistance management practices for diamides in RSB and other arthropods.

Assessment of the Dynamic Exposure to PM2.5 Based on Hourly Cell Phone Location and Land Use Regression Model in Beijing.

The spatiotemporal locations of large populations are difficult to clearly characterize using traditional exposure assessment, mainly due to their complicated daily intraurban activities. This study aimed to extract hourly locations for the total population of Beijing based on cell phone data and assess their dynamic exposure to ambient PM2.5. The locations of residents were located by the cellular base stations that were keeping in contact with their cell phones. The diurnal activity pattern of the total population was investigated through the dynamic spatial distribution of all of the cell phones. The outdoor PM2.5 concentration was predicted in detail using a land use regression (LUR) model. The hourly PM2.5 map was overlapped with the hourly distribution of people for dynamic PM2.5 exposure estimation. For the mobile-derived total population, the mean level of PM2.5 exposure was 89.5 µg/m3 during the period from 2013 to 2015, which was higher than that reported for the census population (87.9 µg/m3). The hourly activity pattern showed that more than 10% of the total population commuted into the center of Beijing (e.g., the 5th ring road) during the daytime. On average, the PM2.5 concentration at workplaces was generally higher than in residential areas. The dynamic PM2.5 exposure pattern also varied with seasons. This study exhibited the strengths of mobile location in deriving the daily spatiotemporal activity patterns of the population in a megacity. This technology would refine future exposure assessment, including either small group cohort studies or city-level large population assessments.

Economic and environmental feasibility of hydrometallurgical process for recycling waste mobile phones.

Waste mobile phones contain significant amounts of valuable metals and non-metallic materials. Consequently, the extraction of valuable materials from discarded phones, which is a more cost-effective method compared with primary mining, is an essential step for maximizing the recovery of secondary resources and minimizing e-waste pollution. We designed a green and efficient path for recovering valuable metals from waste mobile phones and explored its technical feasibility from both environmental and economic perspectives through life cycle assessment and revenue expenditure model. The results showed that the hydrometallurgical process had three characteristics of high recovery efficiency, significant environmental friendliness and economic feasibility. The recovery efficiencies of valuable metals were higher than 90%. Simultaneously, the return on investment was 29%, indicating that the recycling enterprises can achieve self-sufficiency. Thirdly, the environmental benefits were more significant compared to environmental damage released by hydrometallurgical process, representing a significant environmental friendliness. Within the overall recycling process, the core process made the greatest contribution to the environmental burden (45.38-65.68%), followed by manual disassembly process. A comparison of sub-processes in core process revealed that the mechanical crushing and sorting phase had the greatest environmental impacts that were primarily attributed to power consumption. Consequently, future research should focus on the development of energy-efficient pretreatment techniques and energy-saving equipment. The industrial practice of recycling waste mobile phones is still in its infancy in China. Future studies should also focus on the comparing different treatment processes, with the aim of providing technical support for the advancement of industry.

Water-dispersible astaxanthin-rich nanopowder: preparation, oral safety and antioxidant activity in vivo.

In this research, astaxanthin-rich nanopowder was prepared by nanoencapsulation and freeze-drying techniques with enhanced bioavailability and antioxidant activities. The nanopowder showed a maximum solubility of 230 mg mL-1 with an astaxanthin content as high as 2.9%. Compared with free astaxanthin, the astaxanthin-loaded nanopowder exhibited a more efficient antioxidant effect: an oral dose of 0.9 mg per kg BW significantly reduced the malondialdehyde and protein carbonyl contents, and increased the glutathione content as well as the superoxide dismutase activities in alcohol-induced acute hepatic injured mice, and maintained these oxidative stress indicators at a normal level for a longer period when treated with nanoencapsulated-astaxanthin than free astaxanthin. Simulated gastrointestinal tract studies demonstrated that the nanopowder with pH and DNase I-dependent dissociation properties delivered astaxanthin efficiently to the small intestine. Astaxanthin-rich nanopowder with a dose as high as 2.4 mg per kg BW (equivalent to astaxanthin) showed no chronic toxicity to mice in terms of hematology and pathological histology, indicating its impressive biocompatibility for biomedical applications. Pharmacokinetics and relative bioavailability (207%) of the nanopowder further proved that DNA/chitosan nanocarriers significantly improved the delivery efficiency of astaxanthin. With enhanced bioavailability and antioxidant activities, this novel type of astaxanthin-loaded nanopowder is expected to find broad application in the food and drug industry.

Recent advances in microbial production of mannitol: utilization of low-cost substrates, strain development and regulation strategies.

Mannitol has been widely used in fine chemicals, pharmaceutical industries, as well as functional foods due to its excellent characteristics, such as antioxidant protecting, regulation of osmotic pressure and non-metabolizable feature. Mannitol can be naturally produced by microorganisms. Compared with chemical manufacturing, microbial production of mannitol provides high yield and convenience in products separation; however the fermentative process has not been widely adopted yet. A major obstacle to microbial production of mannitol under industrial-scale lies in the low economical efficiency, owing to the high cost of fermentation medium, leakage of fructose, low mannitol productivity. In this review, recent advances in improving the economical efficiency of microbial production of mannitol were reviewed, including utilization of low-cost substrates, strain development for high mannitol yield and process regulation strategies for high productivity.

A thermodynamic model of microtubule assembly and disassembly.

Microtubules are self-assembling polymers whose dynamics are essential for the normal function of cellular processes including chromosome separation and cytokinesis. Therefore understanding what factors effect microtubule growth is fundamental to our understanding of the control of microtubule based processes. An important factor that determines the status of a microtubule, whether it is growing or shrinking, is the length of the GTP tubulin microtubule cap. Here, we derive a Monte Carlo model of the assembly and disassembly of microtubules. We use thermodynamic laws to reduce the number of parameters of our model and, in particular, we take into account the contribution of water to the entropy of the system. We fit all parameters of the model from published experimental data using the GTP tubulin dimer attachment rate and the lateral and longitudinal binding energies of GTP and GDP tubulin dimers at both ends. Also we calculate and incorporate the GTP hydrolysis rate. We have applied our model and can mimic published experimental data, which formerly suggested a single layer GTP tubulin dimer microtubule cap, to show that these data demonstrate that the GTP cap can fluctuate and can be several microns long.