Low-cost and convenient fabrication of polymer micro/nanopores with the needle punching process and their applications in nanofluidic sensing.

Solid-state micro/nanopores play an important role in the sensing field because of their high stability and controllable size. Aiming at problems of complex processes and high costs in pore manufacturing, we propose a convenient and low-cost micro/nanopore fabrication technique based on the needle punching method. The thin film is pierced by controlling the feed of a microscale tungsten needle, and the size variations of the micropore are monitored by the current feedback system. Based on the positive correlation between the micropore size and the current threshold, the size-controllable preparation of micropores is achieved. The preparation of nanopores is realized by the combination of needle punching and chemical etching. First, a conical defect is prepared on the film with the tungsten needle. Then, nanopores are obtained by unilateral chemical etching of the film. Using the prepared conical micropores, resistive-pulse detection of nanoparticles is performed. Significant ionic current rectification is also obtained with our conical nanopores. It is proved that the properties of micro/nanopores prepared by our method are comparable to those prepared by the track-etching method. The simple and controllable fabrication process proposed here will advance the development of low-cost micro/nanopore sensors.

An intelligent quantification system for fetal heart rhythm assessment: A multicenter prospective study.

BACKGROUND: The motion relationship and time intervals of the pulsed-wave Doppler (PWD) spectrum are essential for diagnosing fetal arrhythmia. However, few technologies currently are available to automatically calculate fetal cardiac time intervals (CTIs). OBJECTIVE: The purpose of this study was to develop a fetal heart rhythm intelligent quantification system (HR-IQS) for the automatic extraction of CTIs and establish the normal reference range for fetal CTIs. METHODS: A total of 6498 PWD spectrums of 2630 fetuses over the junction between the left ventricular inflow and outflow tracts were recorded across 14 centers. E, A, and V waves were manually labeled by 3 experienced fetal cardiologists, with 17 CTIs extracted. Five-fold cross-validation was performed for training and testing of the deep learning model. Agreement between the manual and HR-IQS-based values was evaluated using the intraclass correlation coefficient and Spearman's rank correlation coefficient. The Jarque-Bera test was applied to evaluate the normality of CTIs' distributions, and the normal reference range of 17 CTIs was established with quantile regression. Arrhythmia subset was compared with the non-arrhythmia subset using the Mann-Whitney U test. RESULTS: Significant positive correlation (P <.001) and moderate-to-excellent consistency (P <.001) between the manual and HR-IQS automated measurements of CTIs was found. The distribution of CTIs was non-normal (P <.001). The normal range (2.5th to 97.5th percentiles) was successfully established for the 17 CTIs. CONCLUSIONS: Using our HR-IQS is feasible for the automated calculation of CTIs in practice and thus could provide a promising tool for the assessment of fetal rhythm and function.

Connotation, status, and governance of land ecological security in China's new urbanization: recent advances and future prospects.

The rapid development of China's new urbanization has created favorable conditions for economic growth and social development. Urbanization includes population urbanization and land urbanization, among which land urbanization leads to land ecological security problems. At present, there is a lack of comprehensive understanding of land ecological security in China's new urbanization construction. This paper aims to fill the gap by systematically combing relevant literature on the connotation, status, and governance of land ecological security in China's new urbanization. Literature review shows that China's land ecological security is still at a low level, and the new urbanization construction has significant impacts on land ecological security. Land contamination is the most critical factor threatening land ecological security, and there are differences in the levels of land contamination and types of pollutants in different new urbanization construction forms. According to an example of land ecological security governance with enterprises as the main body and multiple subjects cooperating, the governance of land ecological security needs to integrate a variety of different subjects to coordinate governance. Future research directions should focus on the construction of land ecological security assessment index system, development of land contamination multi-level control technology, and construction of multi-subject collaborative governance model with "government-enterprise-social organization-residents."

[Distribution Characteristics and Risk Assessment of Microplastics in Soil in Danjiangkou Reservoir Area of South-to-North Water Diversion Project].

To explore the occurrence characteristics and influencing factors of microplastics in soil in the Danjiangkou Reservoir area, this study collected soil samples from orchards, dryland, paddy fields, and wetland and identified microplastics in soil via density sorting, microscope observation, and Raman spectrometry. The results showed that microplastics were detected in 64 samples collected in the study area, and the abundance ranged from 645 to 15161 n·kg-1. The spatial distribution of microplastics in the bottom of the reservoir was higher than those in the middle and head of the reservoir, and the abundance of microplastics in the surface soil(0-20 cm) was significantly lower than that in the bottom soil(20-40 cm). The main types of microplastics were polypropylene(26.4%) and polyamide(20.2%), the particle size was mainly concentrated between 50-500 µm(75%), and the common shape was fragments(66.2%). Correlation analysis showed that soil microplastic abundance was closely related to land use, distance from water and housing, population density, and soil properties. From the perspective of microplastic pollution risk, 72.1% of the region's microplastic polymer risk index was at level Ⅲ and level Ⅳ, and there was a certain risk of microplastic pollution in the Danjiangkou Reservoir area. The results of this study can provide support for risk assessment of microplastics.

Disparity in hospital beds' allocation at the county level in China: an analysis based on a Health Resource Density Index (HRDI) model.

BACKGROUND: As approximately 3/4 of the population lives in county-level divisions in China, the allocation of health resources at the county level will affect the realization of health equity. This study aims to evaluate the disparity in hospital beds at the county level in China, analyze its causes, and discuss measures to optimize the allocation. METHODS: Data were drawn from the Chinese County/City Statistical Yearbook (2001-2020). The health resource density index (HRDI) was applied to mediate between the influence of demographic and geographical factors on the allocation of hospital beds. The trends of HRDI allocation were evaluated through the growth incidence curve and the probability density function. The regional disparity in the HRDI was examined through the Lorenz curve, and Dagum Gini coefficient. The contribution of the Gini coefficient and its change were assessed by using the Dagum Gini decomposition method. RESULTS: From 2000 to 2019, the number of hospital beds per thousand people at the county level in China increased dramatically by 1.49 times. From the aspect of the HRDI, there were large regional disparities at the national level, with a Gini coefficient of 0.367 in 2019 and in the three subregions. In 2019, the Gini coefficient of the HRDI exhibited regional variations, with the highest value observed in the western region, followed by the central region and the eastern region. Decomposition reveals that the contribution of interregional disparity changed from the dominant factor to the least important factor, accounting for 29.79% of the overall disparity and the contribution of trans-variation intensity increased from 29.19% to 39.75%, whereas the intraregional disparity remained stable at approximately 31% and became the second most important factor. CONCLUSION: The regional disparity in hospital beds allocation at the county level in China was large and has not improved substantially. Trans-variation intensity was the main reason for the overall disparity and changes, and the intraregional disparity was more important than the interregional disparity for the overall disparity.

Study on sewage characteristics in rural China and pollutants removal performance of biologically enhanced internal circulation treatment system.

With rapid economic development and urbanization in China, rural wastewater treatment has become an important issue. This study investigated 63 rural sewage treatment stations in northern, central and southern Shaanxi, China for a 1-year period, 2021 to 2022. The main purpose of the research was to investigate the quality and discharge characteristics of rural sewage, along with current problems in rural wastewater treatment, in order to provide evidence for the optimal construction and operation of rural sewage treatment stations. We found that the biodegradability of rural wastewater is adequate, and BOD5/COD ratio in sewage was 0.4, which is suitable for biological treatment. It has obvious intermittent flow cut-off characteristics, and the range of cut-off duration of sewage was 6-16 h/d, which leads to poor pollutant removal efficiency (COD: 50.0 ± 29.2%, NH4+-N: 46.0 ± 26.1%, TN: 38.5 ± 24.9% and TP: 38.3 ± 23.8%) in sewage treatment stations. In response to the above characteristics, the rural sewage biologically enhanced internal circulation treatment (BEICT) system was constructed. After 97 days of operation, the system has a stable removal effect on TN and TP with an average removal rate of 77.42% and 89.69%, respectively, under the condition of influent interruption for 12 h per day. The activated sludge of system maintained good activity and stable sedimentation performance during the whole experiment, with MLVSS/MLSS and SVI of 0.72 and 128 mL/g, respectively. This study can provide the basis and technical support for the accurate design of rural sewage treatment facilities, and has important significance for guiding the treatment of rural domestic sewage in China.

Distribution, sources, and risk assessment of polycyclic aromatic hydrocarbons (PAHs) in Kaokaowusu river sediments near a coal industrial zone.

This study systematically analyzed the contents, compositions, and sources of polycyclic aromatic hydrocarbons (PAHs) in river sediments near an important energy and chemical base in northwest China. In addition, their possible adverse effects on the ecology and human health were assessed. The PAH concentrations in this study area ranged from 2641.28 to 16783.72 (ng/g dw). PAHs of medium molecular weight (3-ring and 4-ring) showed the largest proportion, followed by PAHs of higher molecular weight (5-ring and 6-ring). The results of molecular diagnostic ratios and principal component analysis revealed that PAHs in the region have complex sources, with incomplete combustion of local fossil fuels and traffic exhaust factors being the main sources. The total toxic equivalent concentration of PAHs varied from 10.05 to 760.26 ng/g, and according to the sediment quality guidelines, PAHs have high potential ecological risk in the lower reaches of the river. The mean effect range-median quotient for the region was 0.46, and the combined ecological risk was at moderate to high levels (21% probability of toxicity). The lifetime carcinogenic risks for adults and children exposed to PAHs were 2.95 × 10-3 and 1.87 × 10-2, respectively, which are much higher than the limit of 10-4, indicating moderate to high potential cancer risks. Therefore, the local government should consider taking some environmental remediation measures. This study can provide theoretical support for pollution prevention measures and ecological restoration strategies for rivers in resource-rich areas.

How does public environmental supervision affect the industrial structure optimization?

Based on the panel data of 278 prefecture-level cities in China from 2005 to 2017, this study takes the environmental information disclosure policy implemented in 2008 as a quasi-natural experiment and uses the difference-in-difference method to examine the impact of public environmental supervision on industrial structure upgrading. This study found that public environmental supervision has indeed significantly promoted the industrial structure optimization. It is noteworthy that, the impact of public environmental supervision on industrial structure optimization is heterogeneous across regions and corresponding to the intensity of environmental regulations. Public environmental supervision in the eastern region has significantly promoted the optimization of the industrial structure, while the promotion effect in the central and western regions is not obvious. Besides, the effect of public environmental supervision is more significant in the cities with relatively tight traditional environmental regulations. The mechanism analysis shows that public environmental supervision promotes industrial structure upgrading mainly by influencing the level of urban technological innovation, and the level of technological innovation plays an intermediary role between public environmental supervision and industrial structure optimization. This study has important reference significance for further improving the environmental information disclosure system and constructing an environmental governance system with the government as the leading body, enterprises as the main body, and the public as the common participation.

Assessing the Views and Needs of People at High Risk of Gestational Diabetes Mellitus for the Development of Mobile Health Apps: Descriptive Qualitative Study.

BACKGROUND: Early prevention of gestational diabetes mellitus (GDM) can reduce the incidence of not only GDM, but also adverse perinatal pregnancy outcomes. Moreover, it is of great significance to prevent or reduce the occurrence of type 2 diabetes. Mobile health (mHealth) apps can help pregnant women effectively prevent GDM by providing risk prediction, lifestyle support, peer support, professional support, and other functions. Before designing mHealth apps, developers must understand the views and needs of pregnant women, and closely combine users' needs to develop app functions, in order to better improve user experience and increase the usage rate of these apps in the future. OBJECTIVE: The objective of this study was to understand the views of the high-risk population of gestational diabetes mellitus on the development of mobile health apps and the demand for app functions, so as to provide a basis for the development of gestational diabetes mellitus prevention apps. METHODS: Fifteen pregnant women with at least one risk factor for gestational diabetes were recruited from July to September 2021, and were interviewed via a semistructured interview using the purpose sampling method. The transcribed data were analyzed by the traditional content analysis method, and themes were extracted. RESULTS: Respondents wanted to develop user-friendly and fully functional mobile apps for the prevention of gestational diabetes mellitus. Pregnant women's requirements for app function development include: personalized customization, accurate information support, interactive design, practical tool support, visual presentation, convenient professional support, peer support, reasonable reminder function, appropriate maternal and infant auxiliary function, and differentiated incentive function.These function settings can encourage pregnant women to improve or maintain healthy living habits during their use of the app. CONCLUSIONS: This study discusses the functional requirements of target users for gestational diabetes mellitus prevention apps, which can provide reference for the development of future applications.

Polycyclic aromatic hydrocarbons (PAHs) in gas, PM2.5, and frost samples in a severely polluted rural site of the North China Plain: Distribution, source, and risk assessment.

The process of frost formation has been studied as an important deposition pathway for removing atmospheric pollutants. However, the removal of organic species during frost formation remains unknown. Gas, PM2.5 and frost samples were collected in Wangdu, a severely polluted rural site in the North China Plain, during the winter of 2018. The concentrations, distributions, sources, and ecological and health risks of the 16 PAHs in the samples were determined. The 24 h-averaged concentrations of PAHs in the air (gas + PM2.5) reached 719 ng m-3, indicating that the local atmosphere was heavily contaminated by PAHs in winter. The average concentrations of PAHs in the frost insoluble reached 27.5 µg g-1, indicating the strong ambient PAHs deposition during the frost process. The distribution patterns of individual PAH species indicated that the PAHs in the gas influenced the frost samples, and the sources of PAHs in frost samples were highly similar to those in PM2.5 samples. The calculated lifetime lung cancer risk (LLCR) via inhalation of ambient PAHs was classified as high risks. The average total Benzo(a)pyrene toxic equivalent quotient (TEQ) of PAHs in the frost samples was estimated as 2.50 µg TEQ g-1, posing a high threat to the ecological environment and health. After the melting of frost, the PAHs are released into the soil. Although the concentrations are diluted, the PAH concentrations could adversely affect the growing vegetation or agricultural activities. The extremely high PAH concentrations and their ecological and health risks require special attention and strict regulation.

IoT terminal security assessment system based on improved assessment method.

The Internet of Things (IoT) technology is widely used and has been improved in research. However, due to the extensiveness of IoT technology, the heterogeneity and diversity of the device structure, the number of attacks against IoT has increased dramatically, so we need a method that can effectively and actively determine safety. Considering the diversity of the terminal structure of IoT, a security method for the IoT terminal based on structural balance, method objectivity, and reliability is currently a challenging task. This paper introduces the idea of rate of change in mathematics into trust analysis, and forms three attribute sets based on trust interval and rate of change: discrete interval, change range, and change frequency. By calculating the above attributes of the entity's trust value, the entity's trust situation is obtained, and an overall assessment of the terminal entity's trust situation is made from the three levels of completeness, accuracy and objectivity. Under the premise of reducing encryption and other means, the above method can evaluate the trust state of the IoT terminal from the perspective of the data, and this evaluation method can provide a basis for the judgment of the IoT terminal more objectively and accurately.

[Risk assessment of excess mortality attribute to PM_(2.5) exposure in 5 cities in China in 2018].

OBJECTIVE: To assess the risk of excess mortality caused by exposure to PM_(2.5) in 5 cities in China. METHODS: Surveillance data on daily concentration of PM_(2.5), meteorology and death-causes were collected from 2015 to 2018 in Harbin, Xi& apos; an, Nanjing, Chengdu and Guangzhou cities. Generalized additive model(GAM) was used to analyze acute exposure-response coefficients of PM_(2.5) exposure on the mortality. Using daily 24-hour average concentration of PM_(2.5) in 2018 to estimate the exposure levels of the population, with PM_(2.5) 24-hour average concentration limit of the ambient air quality standard(GB 3095-2012) for reference concentration(75 µg/m~3), and applicating the health risk assessment model, the excess deaths attribute to PM_(2.5) exposure in the 5 cities in 2018 was estimated. RESULTS: Attribute to PM_(2.5) exposure, the excess cases of death caused by circulatory system diseases in Harbin and Guangzhou were 35 and 92, the excess cases caused by respiratory diseases in Xi& apos; an was 70, the excess cases of caused by non-accident in Nanjing was 69, and the excess cases caused by non-accidental, respiratory and circulatory diseases in Chengdu were 588, 210 and 134 in 2018. CONCLUSION: PM_(2.5) exposure could increase the excess mortality risk, which varies among different cities.

Spatial and Temporal Differences in the Green Efficiency of Water Resources in the Yangtze River Economic Belt and Their Influencing Factors.

Using panel data from 11 regions (9 provinces and two cities) in the Yangtze River Economic Belt (YREB) during 2002-2017, the regional differences in and spatial characteristics of the green efficiency of water resources along the YREB were analyzed. The undesirable outputs slacks-based measure-data envelopment analysis, Malmquist index, and social network analysis models were employed. A dynamic panel using a system generalized method of moments model was established to empirically examine the main factors influencing green efficiency. The results show the following. First, temporally, green efficiency fluctuates while showing an overall decreasing trend; spatially, green efficiency generally decreases in this order: downstream, upstream, then midstream. Second, the change in the total factor productivity (TFP) index shows an overall increasing trend, with TFP improvement mainly attributable to technology. Third, green efficiency shows a significant spatial correlation. All provinces are in the spatial correlation network, and the network, as a whole, has strong stability. Finally, water resource endowment, water prices, government environmental control strength, and the water resources utilization structure have a significant impact on green efficiency.

Assessment of conbercept therapy for high myopia macular neovascularization by optical coherence tomography angiography.

This study aimed to evaluate the efficacy and safety of the intravitreal injection of conbercept in the treatment of macular neovascularization (MNV) secondary to high myopia and to observe the application of optical coherence tomography angiography (OCTA) in the treatment follow-up. We reviewed the medical records of 20 patients (21 eyes) with MNV secondary to high myopia who were enrolled in the Department of Ophthalmology of the First Hospital of China Medical University between May 2018 and January 2020. Each patient received one or more intravitreal injections of conbercept (0.5 mg/0.05 mL). The treatment was conducted according to a 1 + PRN (pro re nata) regimen. The changes of best corrected visual acuity (BCVA), central macular thickness (CMT), and selected MNV and flow areas measured by OCTA were observed over a 6-month follow-up period. The mean logarithm of the minimum angle of resolution (logMAR) BCVA was 1.03 ± 0.61 before treatment and improved to 0.83 ± 0.59 (P = 0.007), 0.78 ± 0.62 (P = 0.001), 0.81 ± 0.73 (P = 0.027), and 0.79 ± 0.72 (P = 0.023) at 1 month, 2 months, 3 months, and 6 months after treatment, respectively. The mean CMT was 358.16 ± 206.11 µm before treatment and decreased to 295.38 ± 178.70 µm (P = 0.003), 288.34 ± 165.60 µm (P = 0.004), 284.36 ± 163.07 µm (P = 0.005), and 283.00 ± 160.32 µm (P = 0.004) at 1 month, 2 months, 3 months, and 6 months after treatment, respectively. Nineteen eyes (90.5%) had stable or improved vision at 6 months of follow-up. One month after conbercept injection, in OCTA images, the small-diameter blood vessels of the MNV decreased, the intertwined small blood vessels decreased or even disappeared, and the main or larger-diameter blood vessels were still present. The mean selected MNV and blood flow areas were 0.62 ± 0.81 and 0.22 ± 0.27 mm2, respectively, before treatment and decreased to 0.23 ± 0.33 and 0.07 ± 0.08 mm2 (P = 0.04 for both), respectively, 1 month after treatment. No drug-related systemic or ocular adverse effects were observed. Our results suggest that conbercept can effectively and safely improve BCVA and reduce CMT in patients with myopic MVN (mMNV). OCTA can be used to observe MNV area, blood flow area, and MNV morphological changes after treatment with conbercept, thus providing a reference for treatment follow-up.

[Assessment of the endangered status and conservation priorities for the rare and endangered plant species in Inner Mongolia, China]. / å† è’™å¤çç¨€æ¿’å±æ¤ç‰©æ¿’å±çŽ°çŠ¶åŠä¼˜å ˆä¿æŠ¤è¯„ä¼°.

With 127 rare and endangered plant species of Inner Mongolian in Catalogue of Rare and Endangered Plants in China, China Plant Red Book, National Key Protected Wild Plants List (List No. 1), China Species Red List, Red List of Biodiversity in China: Volume of Higher Plants, List of Rare and Endangered Plants in Inner Mongolia and Atlas of Rare and Endangered Plants in Inner Mongolia as objects, an evaluation system of the threatened grades and conservation priority of rare and endangered plants were built based on data collection and consultation with experts. We set the five criteria, including endangered coefficient, genetic coefficient, utilization coefficient, habitat coefficient, and reproduction coefficient, under which there were 17 subordinate indicators. The analytic hierarchy process was employed to determine the weight of indicators in the system and calculate the endangered grades and conservation priority grades for the rare and endangered species. According to the results of evaluations, two critically endangered (CR) species, 13 endangered (EN) species, 37 vulnerable (VU) species, 44 near threatened (NT) species, and 31 least concern (LC) species were identified, accounting for 1.6%, 10.2%, 29.1%, 34.7% and 24.4% of the total, respectively. Among those species, 52 species were threatened, namely CR, EN and VU species, accounting for 40.9% of the total. The evaluation results of conservation priority grades were: 35 species of Class 1 protected plants, 72 species of Class 2 protected plants, and 20 species of Class 3 protected plants, accounting for 27.6%, 56.7% and 15.8% of the total, respectively. According to the results of evaluation comparison between Red List of Biodiversity in China: Volume of Higher Plants and List of Rare and Endangered Plants in Inner Mongolia, endangered grades of 75 plant species and the protection classes of 62 plant species were calibrated. In this evaluation, the endangered grades of nine plant species and the protection classes of 32 plant species were newly added.

Predicting medication nonadherence risk in a Chinese inflammatory rheumatic disease population: development and assessment of a new predictive nomogram.

PURPOSE: The aim of this study was to develop and internally validate a medication nonadherence risk nomogram in a Chinese population of patients with inflammatory rheumatic diseases. PATIENTS AND METHODS: We developed a prediction model based on a training dataset of 244 IRD patients, and data were collected from March 2016 to May 2016. Adherence was evaluated using 19-item Compliance Questionnaire Rheumatology. The least absolute shrinkage and selection operator regression model was used to optimize feature selection for the medication nonadherence risk model. Multivariable logistic regression analysis was applied to build a predicting model incorporating the feature selected in the least absolute shrinkage and selection operator regression model. Discrimination, calibration, and clinical usefulness of the predicting model were assessed using the C-index, calibration plot, and decision curve analysis. Internal validation was assessed using the bootstrapping validation. RESULTS: Predictors contained in the prediction nomogram included use of glucocorticoid (GC), use of nonsteroidal anti-inflammatory drugs, number of medicine-related questions, education level, and the distance to hospital. The model displayed good discrimination with a C-index of 0.857 (95% confidence interval: 0.807-0.907) and good calibration. High C-index value of 0.847 could still be reached in the interval validation. Decision curve analysis showed that the nonadherence nomogram was clinically useful when intervention was decided at the nonadherence possibility threshold of 14%. CONCLUSION: This novel nonadherence nomogram incorporating the use of GC, the use of nonsteroidal anti-inflammatory drugs, the number of medicine-related questions, education level, and distance to hospital could be conveniently used to facilitate the individual medication nonadherence risk prediction in IRD patients.

Effect of Dietary Types on Feed Intakes, Growth Performance and Economic Benefit in Tibetan sheep and Yaks on the Qinghai-Tibet Plateau during Cold Season.

Pastoralists on the Tibetan alpine rangeland suffered great economic loss in cold season, due to serious live-weight loss of domestic livestock under traditional grazing management. This study aimed to evaluate the effect of dietary types (crude protein levels) on feed intakes, growth performance and economic returns of local Tibetan sheep and yaks during cold season. Twenty-four yearling Tibetan sheep (25.29±3.95 kg LW) and twenty two-year-old yaks (100.62±4.55 kg LW) with familiar body conditions were randomly assigned to four groups, fed oats hay (OH), oats silage (OS), total mixed ration (TMR) and traditionally grazed on the local cool-season pasture (TG), respectively, over a 135-day experiment. Daily dry matter intake was determined; all animals were weighed at the beginning and every 15 days of the 135-day experiment. Then, the total live-weight gain, average daily live-weight gain, gain rate, feed efficiency and net economic benefit were calculated. Results indicated that feed and nutrient intakes (DMI, DMI/kg LW, DMI/kg LW0.75 and CPI) of TMR, OH and OS were higher than TG (P < 0.05). Grazing animals suffered serious live-weight loss, while TMR, OS and OH significantly (P < 0.05) improved total live-weight gain and gain rate in both Tibetan sheep and yaks during the entire experiment. TMR worked better in animal performance and feed efficiency, obtained the highest breeding profit in both Tibetan sheep and yaks among four treatments (P < 0.05). When expressed on net economic benefit, TMR shared the highest net economic benefit in Tibetan sheep, OH shared the highest net economic benefit in yaks, but, no significant difference of net economic benefit in yaks fed TMR and OH diets was determined (P > 0.05). Results indicated that TMR was a reasonable diet in promoting feed intakes, animal performance, feed efficiency and economic returns in domestic livestock, which should be considered by local herdsmen to increase their breeding profit during cold season.

Accelerated life assessment of coating on the radar structure components in coastal environment.

AIMS: This paper aimed to build an accelerated life test scheme and carry out quantitative analysis between accelerated life test in the laboratory and actual service for the coating composed of epoxy primer and polyurethane paint on structure components of some kind of radar served in the coastal environment of South China Sea. METHODS: The accelerated life test scheme was built based on the service environment and failure analysis of the coating. The quantitative analysis between accelerated life test and actual service was conducted by comparing the gloss loss, discoloration, chalking, blistering, cracking and electrochemical impedance spectroscopy of the coating. RESULTS: The main factors leading to the coating failure were ultraviolet radiation, temperature, moisture, salt fog and loads, the accelerated life test included ultraviolet radiation, damp heat, thermal shock, fatigue and salt spray. The quantitative relationship was that one cycle of the accelerated life test was equal to actual service for one year. CONCLUSIONS: It was established that one cycle of the accelerated life test was equal to actual service for one year. It provided a precise way to predict actual service life of newly developed coatings for the manufacturer.

Early myocardial damage assessment in dystrophinopathies using (99)Tc(m)-MIBI gated myocardial perfusion imaging.

BACKGROUND: Early detection of muscular dystrophy (MD)-associated cardiomyopathy is important because early medical treatment may slow cardiac remodeling and attenuate symptoms of cardiac dysfunction; however, no sensitive and standard diagnostic method for MD at an earlier stage has been well-recognized. Thus, the aim of this study was to test the early diagnostic value of technetium 99m-methoxyisobutylisonitrile ((99)Tc(m)-MIBI) gated myocardial perfusion imaging (G-MPI) for MD. METHODS AND RESULTS: Ninety-one patients underwent (99)Tc(m)-MIBI G-MPI examinations when they were diagnosed with Duchenne muscular dystrophy (DMD) (n=77) or Becker muscular dystrophy (BMD; n=14). (99)Tc(m)-MIBI G-MPI examinations were repeated in 43 DMD patients who received steroid treatments for 2 years as a follow-up examination. Myocardial defects were observed in nearly every segment of the left ventricular wall in both DMD and BMD patients compared with controls, especially in the inferior walls and the apices by using (99)Tc(m)-MIBI G-MPI. Cardiac wall movement impairment significantly correlated with age in the DMD and BMD groups (r s=0.534 [P<0.05] and r s=0.784 [P<0.05], respectively). Intermittent intravenous doses of glucocorticoids and continuation with oral steroid treatments significantly improved myocardial function in DMD patients (P<0.05), but not in BMD patients. CONCLUSION: (99)Tc(m)-MIBI G-MPI is a sensitive and safe approach for early evaluation of cardiomyopathy in patients with DMD or BMD, and can serve as a candidate method for the evaluation of progression, prognosis, and assessment of the effect of glucocorticoid treatment in these patients.

Joint Associations of Diet, Lifestyle, and Genes with Age-Related Macular Degeneration.

PURPOSE: Unhealthy lifestyles have been associated with increased odds for age-related macular degeneration (AMD). Whether this association is modified by genetic risk for AMD is unknown and was investigated. DESIGN: Interactions between healthy lifestyles AMD risk genotypes were studied in relation to the prevalence of AMD, assessed 6 years later. PARTICIPANTS: Women 50 to 79 years of age in the Carotenoids in Age-Related Eye Disease Study with exposure and AMD data (n=1663). METHODS: Healthy lifestyle scores (0-6 points) were assigned based on Healthy Eating Index scores, physical activity (metabolic equivalent of task hours/week), and smoking pack years assessed in 1994 and 1998. Genetic risk was based on Y402H in complement factor H (CFH) and A69S in age-related maculopathy susceptibility locus 2 (ARMS2). Additive and multiplicative interactions in odds ratios were assessed using the synergy index and a multiplicative interaction term, respectively. MAIN OUTCOME MEASURES: AMD presence and severity were assessed from grading of stereoscopic fundus photographs taken in 2001-2004. AMD was present in 337 women, 91% of whom had early AMD. RESULTS: The odds of AMD were 3.3 times greater (95% confidence interval [CI], 1.8-6.1) in women with both low healthy lifestyle score (0-2) and high-risk CFH genotype (CC), relative to those who had low genetic risk (TT) and high healthy lifestyle scores (4-6). There were no significant additive (synergy index [SI], 1.08; 95% CI, 0.70-1.67) or multiplicative (Pinteraction=0.94) interactions in the full sample. However, when limiting the sample to women with stable diets before AMD assessment (n=728) the odds for AMD associated with low healthy lifestyle scores and high-risk CFH genotype were strengthened (odds ratio, 4.6; 95% CI, 1.8-11.6) and the synergy index was significant (SI, 1.34; 95% CI, 1.05-1.70). Adjusting for dietary lutein and zeaxanthin attenuated, and therefore partially explained, the joint association. There were no significant additive or multiplicative interactions for ARMS2 and lifestyle score. CONCLUSIONS: Having unhealthy lifestyles and 2 CFH risk alleles increased AMD risk (primarily in the early stages), in an or additive or greater (synergistic) manner. However, unhealthy lifestyles increased AMD risk regardless of AMD risk genotype.