In Vitro Assessment on Designing Novel Antibiofilms of Pseudomonas aeruginosa Using a Computational Approach.

An anti-biofilm that can inhibit the matrix of biofilm formation is necessary to prevent recurrent and chronic Pseudomonas aeruginosa infection. This study aimed to design compounds with a new mechanism through competitive inhibitory activity against phosphomannomutase/phosphoglucomutase (PMM/PGM), using in vitro assessment and a computational (in silico) approach. The active site of PMM/PGM was assessed through molecular redocking using L-tartaric acid as the native ligand and other small molecules, such as glucaric acid, D-sorbitol, and ascorbic acid. The docking program set the small molecules to the active site, showing a stable complex formation. Analysis of structural similarity, bioavailability, absorption, distribution, metabolism, excretion, and toxicity properties proved the potential application of ligands as an anti-biofilm. In vitro assessment with crystal violet showed that the ligands could reach up to 95.87% inhibition at different concentrations. The nitrocellulose membrane and scanning electron microscopic visualization showed that the untreated P. aeruginosa biofilm was denser than the ligand-treated biofilm.

Ultra-fast RP-HPLC-FD-DAD for quantification of total aflatoxins in maize, rice, wheat, peanut and poultry feed without sample clean up, and population exposure risk assessment in Katsina, Nigeria: an optimization study.

This study reports the development and validation of a simple, yet efficient method called the ultra-fast reverse phase high-performance liquid chromatography with fluorescence and photodiode array detector (UF-RP-HPLC-FD-DAD) to extract and quantify the total aflatoxin from grains and poultry feed. The proposed method is used to determine the total aflatoxin content in 150 samples of maize, rice, wheat, peanut and poultry feed obtained from open markets in a state in Nigeria. The extent of consumer exposure to aflatoxins and the risk of developing hepatocellular carcinoma (HCC) are evaluated. The UF-RP-HPLC-FD-DAD method was found to be satisfactorily accurate, sensitive and reliable as ascertained by its excellent validation outcomes (R2 > 0.999, LoD < 0.08 ng g-1, LoQ < 0.2 ng g-1, recovery = 90-102%). The aflatoxin levels in food grains and poultry feed samples obtained in this study implied a moderate dietary exposure of between 10.67 and 20.77 ng/kg BW/day, in which the risk of developing HCC was estimated to be between 6.27 and 21.40% per 100,000 adults/year. Hence, greater monitoring of marketed food and feed is required, besides the deployment of strict controls and preventive techniques to minimize the population's exposure to a high dietary level of aflatoxins.