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J Cyst Fibros ; 18(6): 808-816, 2019 11.
Artigo em Inglês | MEDLINE | ID: mdl-30905581

RESUMO

BACKGROUND: Routine clinical culture detects a subset of the cystic fibrosis (CF) airways microbiota based on culture-independent (molecular) methods. This study aimed to determine how extended sputum culture of viable bacteria changes over time in relation to clinical status and predicts exacerbations. METHODS: Sputa from patients at a baseline stable and up to three subsequent time-points were analysed by extended-quantitative culture; aerobe/anaerobe densities, ecological indexes and community structure were assessed together with clinical outcomes. RESULTS: Eighty patients were prospectively recruited. Sputa were successfully collected and cultured at 199/267 (74.5%) study visits. Eighty-two sputa from 25 patients comprised a complete sample-set for longitudinal analyses. Bacterial density, ecological indexes and clinical outcomes were unchanged in 18 patients with three sequential stable visits. Conversely, in 7 patients who had an exacerbation, total bacterial and aerobe densities differed over four study visits (P < .001) with this difference particularly apparent between the baseline visit and completion of acute antibiotic treatment where a decrease in density was observed. Bacterial communities were more similar within than between patients but stable patients had the least variation in community structure over time. Using logistic regression in a further analysis, baseline features in 37 patients without compared to 15 patients with a subsequent exacerbation showed that clinical measures rather than bacterial density or ecological indexes were independent predictors of an exacerbation. CONCLUSIONS: Greater fluctuation in the viable bacterial community during treatment of an exacerbation than between stable visits was observed. Extended-quantitative culture did not provide prognostic information of a future exacerbation.


Assuntos
Antibacterianos/uso terapêutico , Biota/efeitos dos fármacos , Contagem de Colônia Microbiana/métodos , Fibrose Cística , Microbiota/efeitos dos fármacos , Escarro/microbiologia , Avaliação de Sintomas , Adolescente , Adulto , Criança , Fibrose Cística/diagnóstico , Fibrose Cística/tratamento farmacológico , Fibrose Cística/microbiologia , Progressão da Doença , Feminino , Humanos , Pulmão/microbiologia , Masculino , Gravidade do Paciente , Prognóstico , Avaliação de Sintomas/métodos , Avaliação de Sintomas/estatística & dados numéricos , Exacerbação dos Sintomas
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