Life cycle assessment and technoeconomic analysis of biofuels produced from polyculture microalgae cultivated in greywater.

This study evaluated the environmental and economic impacts of substituting synthetic media with greywater for cultivating microalgae in the biofuel production process. Life cycle assessment (LCA) and technoeconomic assessment (TEA) were employed to compare the impacts of two scenarios - one containing bold's basal (BB) media and another containing greywater as growth mediums for microalgae cultivation. Scenarios 1 and 2 mitigated 1.74 and 2.14 kg CO2 per kg of biofuel production, respectively. Substituting BB media with greywater resulted in a 16.3% reduction in energy requirements, leading to a 79.3% increase in net energy recovered. LCA findings demonstrate a reduction in all seven environmental categories. TEA reveals that, despite a 21.7% higher capital investment, scenario 2 proves more economically viable due to a 39.8% lower operating cost and additional revenue from wastewater treatment and carbon credits. The minimum selling price of biofuel dropped from Rs 73.5/kg to Rs 36.5/kg, highlighting the economic and environmental advantages of substituting BB media with greywater in microalgal biofuel production.

Low-Cost Greywater Treatment Using Polyculture Microalgae-Microalgal Growth, Organics, and Nutrient Removal Subject to pH and Temperature Variations During the Treatment.

Organics and nutrient removal studies are rarely done using polyculture microalgae, and that too in outdoor conditions, as they are often not deemed effective for wastewater treatment purposes. This study examined the organics and nutrient removal efficiency of polyculture microalgae cultivated in greywater. The reactor was operated in outdoor conditions. Hence, it was subjected to natural pH and temperature variations. A growth rate of 0.05 g L-1 day-1 was observed for temperatures up to 37 °C, beyond which the growth rate declined by 0.07 g L-1 day-1. During the treatment, the pH of the system was observed to be between 7.4 and 8.4. However, the growth rate would again pick up (0.05 g L-1 day-1) when the pH and temperature moved towards the optimum range, indicating that the polycultures adapt very quickly to their environment. The maximum biomass concentration reached 0.82 gL-1. The highest removal efficiency of organic carbon, ammonia, and phosphate was 80.7, 61.9, and 58.4%, respectively. Nitrate and nitrite concentrations remained ≤ 1.3 mgL-1 and ≤ 2 mgL-1, respectively, indicating the absence of nitrification/denitrification and ammonia volatilization. The mass balance of microalgae indicated that the primary removal mechanism of nitrogen and phosphorus removal was assimilation by the microalgae. The study proved polyculture microalgae to be as effective as some monoculture species in wastewater treatment, which require costlier controlled growth conditions. The high organics and nutrient removal by polycultures in outdoor conditions could pave the way to reducing wastewater treatment costs.