Assessment of the environmental impact of an abandoned mine using an integrative approach: A case-study of the "Las Musas" mine (Extremadura, Spain).

The mine abandonment is generally associated with the release of potentially toxic metals into the environment, which may depend on metals speciation, soil properties and climate conditions. The goal of the present work was to assess the environmental impact of the abandoned Pb-Zn mine "Las Musas" (Spain) using an integrative approach. The impact on soils and surface waters was performed using: chemical parameters, quantification of potentially toxic metals (Cd, Cu, Pb and Zn), and ecotoxicological responses using lethal and sub-lethal bioassays with organisms' representative of different trophic level ((soil: Eisenia fetida (mortality and reproduction test); Latuca sativa and Lollium perenne (seedling emergence); and water: Vibrio fischeri (luminescence inhibition), Daphnia magna (immobility and reproduction test), Thamnocephalus platyurus (mortality), Pseudokirchneriella subcapitata (growth inhibition)). The results showed soils with neutral to slight alkaline pH (7.64-8.18), low electric conductivity (125-953 µS/cm) and low organic matter levels (0.20-1.85%). For most of the soil samples, Pb was the only metal which surpassed the limit proposed by the Canadian soil quality guidelines, with values ranging from 42.2 to 181.4 mg/kg. The ecotoxicological results showed that the soils with the highest levels of Pb induced a decrease on E. fetida reproduction and on L. sativa germination, indicating negative impacts on the habitat function. The analysis of the surface waters showed levels of Zn surpassing the legal limit adopted from the Water Framework Directive (37.0 to 69.0 µg/L). The ecotoxicological results highlight the importance of bioassays that evaluate the behavior of species, when assessing the risk of mining areas with non-acid soils and waters with high nutrients/organic matter concentrations and low concentrations of potentially toxic metals. The results indicated a moderate environmental risk from potentially toxic metals, at the areas analyzed around the Azuaga mine.

Organic wastes as soil amendments - Effects assessment towards soil invertebrates.

Using organic wastes, as soil amendments, is an important alternative to landfilling with benefits to soil structure, water retention, soil nutrient and organic matter concentrations. However, this practice should be monitored for its environmental risk due to the frequent presence, of noxious substances to soil organisms. To evaluate the potential of eight organic wastes with different origins, as soil amendments, reproduction tests with four soil invertebrate species (Folsomia candida, Enchytraeus crypticus, Hypoaspis aculeifer, Eisenia fetida) were performed using gradients of soil-waste mixtures. Results obtained demonstrated that contaminant concentrations required by current legislation might not be a protective measure for the soil ecosystem, as they do not properly translate the potential toxicity of wastes to soil invertebrates. Some wastes with contaminant loadings below thresholds showed higher toxicity than wastes with contaminants concentrations above legal limits. Also, test organism reproduction was differently sensitive to the selected wastes, which highlights the need to account for different organism sensitivities and routes of exposure when evaluating the toxicity of such complex mixtures. Finally this study shows that when combining chemical and ecotoxicological data, it is possible to postulate on potential sources of toxicity, contributing to better waste management practices and safer soil organic amendment products.

Ecotoxicological assessment of the potential impact on soil porewater, surface and groundwater from the use of organic wastes as soil amendments.

This study aimed to assess the potential impact on soil porewater, surface and groundwater from the beneficial application of organic wastes to soil, using their eluates and acute bioassays with aquatic organisms and plants: luminescence inhibition of Vibrio fischeri (15 and 30 min), Daphnia magna immobilization (48 h), Thamnocephalus platyurus survival (24 h), and seed germination of Lolium perenne (7 d) and Lactuca sativa (5 d). Some organic wastes' eluates promoted high toxic responses, but that toxicity could not be predicted by their chemical characterization, which is compulsory by regulatory documents. In fact, when organisms were exposed to the water-extractable chemical compounds of the organic wastes, the toxic responses were more connected to the degree of stabilization of the organic wastes, or to the treatment used to achieve that stabilization, than to their contaminant load. That is why the environmental risk assessment of the use of organic wastes as soil amendments should integrate bioassays with eluates, in order to correctly evaluate the effects of the most bioavailable fraction of all the chemical compounds, which can be difficult to predict from the characterization required in regulatory documents. According to our results, some rapid and standardized acute bioassays can be suggested to integrate a Tier 1 ecotoxicological evaluation of organic wastes with potential to be land applied, namely luminescence inhibition of V. fischeri, D. magna immobilization, and the germination of L. perenne and L. sativa.