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1.
BMC Biol ; 21(1): 147, 2023 06 26.
Artigo em Inglês | MEDLINE | ID: mdl-37365558

RESUMO

BACKGROUND: Mesophotic coral communities are increasingly gaining attention for the unique biological diversity they host, exemplified by the numerous mesophotic fish species that continue to be discovered. In contrast, many of the photosynthetic scleractinian corals observed at mesophotic depths are assumed to be depth-generalists, with very few species characterised as mesophotic-specialists. This presumed lack of a specialised community remains largely untested, as phylogenetic studies on corals have rarely included mesophotic samples and have long suffered from resolution issues associated with traditional sequence markers. RESULTS: Here, we used reduced-representation genome sequencing to conduct a phylogenomic assessment of the two dominant mesophotic genera of plating corals in the Indo-Pacific and Western Atlantic, respectively, Leptoseris and Agaricia. While these genome-wide phylogenies broadly corroborated the morphological taxonomy, they also exposed deep divergences within the two genera and undescribed diversity across the current taxonomic species. Five of the eight focal species consisted of at least two sympatric and genetically distinct lineages, which were consistently detected across different methods. CONCLUSIONS: The repeated observation of genetically divergent lineages associated with mesophotic depths highlights that there may be many more mesophotic-specialist coral species than currently acknowledged and that an urgent assessment of this largely unstudied biological diversity is warranted.


Assuntos
Antozoários , Recifes de Corais , Animais , Filogenia , Ecossistema , Antozoários/genética , Biodiversidade
2.
J Clin Microbiol ; 37(5): 1409-14, 1999 May.
Artigo em Inglês | MEDLINE | ID: mdl-10203496

RESUMO

We conducted a multicenter evaluation of commercial and in-house PCR methods for the detection of enteroviruses. Three coded panels of test and control RNA samples, artificial clinical specimens, and representative enterovirus serotypes were used to assess amplification methods, RNA extraction methods, and reactivities with different enterovirus serotypes. Despite several differences between PCR methods, there was good agreement, although some variation in sensitivity was observed. Most PCR methods were able to detect enterovirus RNA derived from 0.01 50% tissue culture infective dose (TCID50) and were able to detect at least 1 TCID50 of enterovirus in cerebrospinal fluid, stool, or throat swab specimens. Most were also able to detect a wide range of enterovirus serotypes, although serotypic identification was not possible. Some laboratories experienced false-positive results due to PCR contamination, which appeared to result mainly from cross-contamination of specimens during RNA extraction. Provided that this problem is overcome, these PCR methods will prove to be a sensitive and rapid alternative to cell culture for the diagnosis of enterovirus infection.


Assuntos
Enterovirus/isolamento & purificação , Reação em Cadeia da Polimerase/normas , Sequência de Bases , Enterovirus/classificação , Humanos , Dados de Sequência Molecular , RNA Viral/isolamento & purificação
3.
Vet Rec ; 130(6): 119-21, 1992 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-1561744

RESUMO

Increases in serum unconjugated bile acid concentrations have recently been shown to be diagnostic for small intestinal bacterial overgrowth in man. Similar increases in serum unconjugated bile acids were detected in three of nine cats with chronic diarrhoea and vomiting.


Assuntos
Ácidos e Sais Biliares/sangue , Doenças do Gato/diagnóstico , Diarreia/veterinária , Síndromes de Malabsorção/veterinária , Vômito/veterinária , Animais , Doenças do Gato/sangue , Gatos , Doença Crônica , Diarreia/sangue , Ingestão de Alimentos , Síndromes de Malabsorção/sangue , Síndromes de Malabsorção/diagnóstico , Valores de Referência , Vômito/sangue , Redução de Peso
4.
Tissue Antigens ; 26(1): 60-70, 1985 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-3875912

RESUMO

FMC3 and FMC29 are monoclonal antibodies which react with a 30,000 Dalton molecule expressed on the lymphocyte surface membrane. The molecule is a protein which does not appear to be N-glycosylated. The antigen, which is also expressed intracellularly, does not appear to be a marker of differentiation or maturation. Polyclonal cell populations, such as peripheral blood lymphocytes, show a bimodal distribution of surface antigen density, whilst monoclonal cell populations analysed quantitatively showed unimodal antigen density distributions. This suggests the antigen may be a clonal marker.


Assuntos
Leucócitos/imunologia , Anticorpos Monoclonais/imunologia , Antígenos de Superfície/imunologia , Antígenos de Superfície/isolamento & purificação , Diferenciação Celular/efeitos dos fármacos , Células Clonais/imunologia , Dimetil Sulfóxido/farmacologia , Humanos , Leucemia/imunologia , Peso Molecular , Fenótipo , Ésteres de Forbol/farmacologia , Linfócitos T/imunologia
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