Sub-optimal host plants have developmental and thermal fitness costs to the invasive fall armyworm.

The fall armyworm (FAW) Spodoptera frugiperda (J.E. Smith) is a global invasive pest of cereals. Although this pest uses maize and sorghum as its main hosts, it is associated with a wide range of host plants due to its polyphagous nature. Despite the FAW's polyphagy being widely reported in literature, few studies have investigated the effects of the non-preferred conditions or forms (e.g., drought-stressed forms) of this pest's hosts on its physiological and ecological fitness. Thus, the interactive effects of biotic and abiotic stresses on FAW fitness costs or benefits have not been specifically investigated. We therefore assessed the effects of host plant quality on the developmental rates and thermal tolerance of the FAW. Specifically, we reared FAW neonates on three hosts (maize, cowpeas, and pearl millet) under two treatments per host plant [unstressed (well watered) and stressed (water deprived)] until the adult stage. Larval growth rates and pupal weights were determined. Thermal tolerance traits viz critical thermal maxima (CTmax), critical thermal minima (CTmin), heat knockdown time (HKDT), chill-coma recovery time (CCRT), and supercooling points (SCPs) were measured for the emerging adults from each treatment. The results showed that suboptimal diets significantly prolonged the developmental time of FAW larvae and reduced their growth rates and ultimate body weights, but did not impair their full development. Suboptimal diets (comprising non-cereal plants and drought-stressed cereal plants) increased the number of larval instars to eight compared to six for optimal natural diets (unstressed maize and pearl millet). Apart from direct effects, in all cases, suboptimal diets significantly reduced the heat tolerance of FAWs, but their effect on cold tolerance was recorded only in select cases (e.g., SCP). These results suggest host plant effects on the physical and thermal fitness of FAW, indicating a considerable degree of resilience against multiple stressors. This pest's resilience can present major drawbacks to its cultural management using suboptimal hosts (in crop rotations or intercrops) through its ability to survive on most host plants despite their water stress condition and gains in thermal fitness. The fate of FAW population persistence under multivariate environmental stresses is therefore not entirely subject to prior environmental host plant history or quality.

Understanding costs and benefits of thermal plasticity for pest management: insights from the integration of laboratory, semi-field and field assessments of Ceratitis capitata (Diptera: Tephritidae).

The relative costs and benefits of thermal acclimation for manipulating field performance of pest insects depend upon a number of factors including which traits are affected and how persistent any trait changes are in different environments. By assessing plastic trait responses of Ceratitis capitata (Mediterranean fruit fly) across three distinct operational environments (laboratory, semi-field, and field), we examined the influence of different thermal acclimation regimes (cool, intermediate [or handling control], and warm) on thermal tolerance traits (chill-coma recovery, heat-knockdown time, critical thermal minimum and critical thermal maximum) and flight performance (mark-release-recapture). Under laboratory conditions, thermal acclimation altered thermal limits in a relatively predictable manner and there was a generally positive effect across all traits assessed, although some traits responded more strongly. By contrast, dispersal-related performance yielded strongly contrasting results depending on the specific operational environment assessed. In semi-field conditions, warm- or cold-acclimated flies were recaptured more often than the control group at cooler ambient conditions suggesting an overall stimulatory influence of thermal variability on low-temperature dispersal. Under field conditions, a different pattern was identified: colder flies were recaptured more in warmer field conditions relative to other treatment groups. This study highlights the trait- and context-specific nature of how thermal acclimation influences traits of thermal performance and tolerance. Consequently, laboratory and semi-field assessments of dispersal may not provide results that extend into the field setting despite the apparent continuum of environmental complexity among them (laboratory < semi-field < field).

Comparative assessment of the thermal tolerance of spotted stemborer, Chilo partellus (Lepidoptera: Crambidae) and its larval parasitoid, Cotesia sesamiae (Hymenoptera: Braconidae).

Under stressful thermal environments, insects adjust their behavior and physiology to maintain key life-history activities and improve survival. For interacting species, mutual or antagonistic, thermal stress may affect the participants in differing ways, which may then affect the outcome of the ecological relationship. In agroecosystems, this may be the fate of relationships between insect pests and their antagonistic parasitoids under acute and chronic thermal variability. Against this background, we investigated the thermal tolerance of different developmental stages of Chilo partellus Swinhoe (Lepidoptera: Crambidae) and its larval parasitoid, Cotesia sesamiae Cameron (Hymenoptera: Braconidae) using both dynamic and static protocols. When exposed for 2 h to a static temperature, lower lethal temperatures ranged from -9 to 6 °C, -14 to -2 °C, and -1 to 4 °C while upper lethal temperatures ranged from 37 to 48 °C, 41 to 49 °C, and 36 to 39 °C for C. partellus eggs, larvae, and C. sesamiae adults, respectively. Faster heating rates improved critical thermal maxima (CTmax ) in C. partellus larvae and adult C. partellus and C. sesamiae. Lower cooling rates improved critical thermal minima (CTmin ) in C. partellus and C. sesamiae adults while compromising CTmin in C. partellus larvae. The mean supercooling points (SCPs) for C. partellus larvae, pupae, and adults were -11.82 ± 1.78, -10.43 ± 1.73 and -15.75 ± 2.47, respectively. Heat knock-down time (HKDT) and chill-coma recovery time (CCRT) varied significantly between C. partellus larvae and adults. Larvae had higher HKDT than adults, while the latter recovered significantly faster following chill-coma. Current results suggest developmental stage differences in C. partellus thermal tolerance (with respect to lethal temperatures and critical thermal limits) and a compromised temperature tolerance of parasitoid C. sesamiae relative to its host, suggesting potential asynchrony between host-parasitoid population phenology and consequently biocontrol efficacy under global change. These results have broad implications to biological pest management insect-natural enemy interactions under rapidly changing thermal environments.

Fitness costs of rapid cold-hardening in Ceratitis capitata.

Rapid cold-hardening (RCH) is a unique form of phenotypic plasticity which confers survival advantages at low temperature. The fitness costs of RCH are generally poorly elucidated and are important to understanding the evolution of plastic physiology. This study examined whether RCH responses, induced by ecologically relevant diel temperature fluctuations, carry metabolic, survival, or fecundity costs. We predicted that potential costs in RCH would be manifested as differences in metabolic rate, fecundity, or survival in flies which have hardened versus those which have not, or flies that have experienced more RCH events would show greater costs than those which have experienced fewer events. One group of flies cooled to 10°C for 2 h for 11 consecutive days experienced daily RCH (Hardened), whereas the other group exposed to 15°C for the same 2-h period each day formed a Control group. Hardened flies had higher survival at -5°C for 2 h than control flies (69 ± 9% vs. 44 ± 19%, P = 0.04). Hardened flies showed no metabolic or fecundity costs, but had reduced average survival (P = 0.0403). Thus, a major cost to repeated low temperature exposures in Ceratitis capitata is through direct mortality caused by chilling injury, although this appears not to be a direct cost of RCH.