Immunohistochemical assessment of the MIB, CK-14, p63, p16, Cal A, and Cys A markers in spindle cell squamous cell carcinomas of the lip / Avaliação imuno-histoquímica dos marcadores MIB, CK-14, p63, p16, Cal A e Cys em carcinomas espinocelulares de lábio

Objective: with the objective of testing the expression of the protein markers MIB, CK14, p63, p16, Cal A, and Cys A in the pathogenesis of oral spindle cell carcinoma, we conducted an immunohistochemical study of the expression of the protein markers MIB, CK14, p63, p16, Cal A, and Cys A in human biopsy specimens of these lesions. Methods: fifteen histological specimens of spindle cell squamous cell carcinoma of the lower lip were obtained from the Department of Oral Pathology, Bahia Federal University. Immunohistochemical analyses were performed at the Molecular Biology Laboratory of the Department of Otorhinolaryngology, Heidelberg University, Germany. Results: statistical analysis revealed no association between markers. There was strong positive staining for Ck14, MIB, and Cal A in 93.3% of cases, thus establishing a strong association. Conclusion: p63, p16, MIB, Cal A, Cys A are markedly expressed and p16 is strongly suppressed in oral cavity tumors, which suggests that the latter protein may play a role in negative regulation of cell cycle progression.

Comparative chemical study of MTA and Portland cements.

Portland cement has been analyzed and compared to mineral trioxide aggregate (MTA) because of their chemical similarity. The possibility of using this material as a less expensive alternative to MTA in dental practice should be considered. In view of this, the present study compared the components of a Portland cement (Votoran) to two commercial brands of MTA (Pro-Root and MTA-Angelus). Twelve specimens of each material were fabricated and examined by scanning electron microscopy (SEM) with energy dispersive spectroscopy (EDS) to obtain their percentage of chemical elements. The means of the chemical elements found in each material was compared by descriptive statistics. Bismuth was present only in MTA cements to provide radiopacity. In conclusion, the tested cements have similar components, which supports, as far as composition is concerned, the possible clinical use of Portland as an option to MTA.

Comparative chemical study of MTA and portland cements

Portland cement has been analyzed and compared to mineral trioxide aggregate (MTA) because of their chemical similarity. The possibility of using this material as a less expensive alternative to MTA in dental practice should be considered. In view of this, the present study compared the components of a Portland cement (Votoran®) to two commercial brands of MTA (Pro-Root™ and MTA-Angelus®). Twelve specimens of each material were fabricated and examined by scanning electron microscopy (SEM) with energy dispersive spectroscopy (EDS) to obtain their percentage of chemical elements. The means of the chemical elements found in each material was compared by descriptive statistics. Bismuth was present only in MTA cements to provide radiopacity. In conclusion, the tested cements have similar components, which supports, as far as composition is concerned, the possible clinical use of Portland as an option to MTA.

O cimento Portland tem sido analisado e comparado com o agregado trióxido mineral (MTA) devido a sua similaridade química. Dada à possibilidade de uso na Odontologia como uma alternativa menos onerosa ao MTA, realizou-se uma análise comparativa dos componentes do cimento Portland (Votoran®) com os constituintes de dois cimentos MTA (Pro-Root™-MTA e Angelus®-MTA). Para tanto, foram confeccionados 12 corpos-de-prova de cada um dos materiais (n=36), e estes foram analisados em microscopia eletrônica de varredura (MEV) pela técnica de espectroscopia por dispersão de energia (EDS), que fornece o percentual dos componentes químicos encontrados nos corpos-de-prova. As médias dos elementos químicos encontrados nos três cimentos foram comparadas por meio de análise estatística descritiva. O bismuto estava presente somente nos cimentos MTA. Concluiu-se que os cimentos testados apresentaram similaridade em seus constituintes, o que indica, considerando-se a composição, a possibilidade de futura utilização clínica do cimento Portland como alternativa ao MTA.

Avaliaçäo do grau de satisfaçäo dos estudantes com o Programa de Pós-Graduaçäo, nível Mestrado e Doutorado, em Cirurgia e Traumatologia Bucomaxilofacial da PUCRS / Satisfaction assessment of the post graduate students enrolled in the oral and maxillofacial surgery program at PUCRS

Os autores realizam, neste trabalho, uma avaliaçäo do grau de satisfaçäo dos estudantes do Programa de Pós-Graduaçäo em Cirurgia e Traumatologia Bucomaxilofacial da PUCRS, através da aplicaçäo de um questionário aos estudantes, investigando seus posicionamentos referentes a estrutura curricular, qualificaçäo do corpo docente, preparo para a docência e pesquisa e desempenho individual discente. As respostas obtidas foram variadas, apontando para uma percepçäo majoritariamente positiva em relaçäo ao Programa