Quantitative microbial risk assessment of gastrointestinal illness due to recreational exposure to E. coli and enterococci on the southern coasts of the Caspian Sea.

Background: Gastrointestinal illness refers to a broad range of diseases that affect the digestive system, including infections caused by bacteria, viruses, and parasites. Quantitative Microbial Risk Assessment (QMRA) is a powerful tool used to evaluate the risks associated with microbial pathogens in various environments. The main objective of this study was to conduct a quantitative assessment of gastrointestinal illnesses that occur as a result of exposure to E. coli and enterococci during recreational activities on the southern coasts of the Caspian Sea. Methods: Samples were collected from the recreational beaches along the border line of the Caspian Sea. The samples were analyzed for the presence and enumeration of E. coli and enterococci using the microplate method and membrane filtration techniques. Then, the annual and daily infection risks were computed using the Monte Carlo simulation approach. Results: The results revealed that the risk of daily and annual infections on the coasts of Babolsar was higher than that on the coasts of Sari. Also, in the recreational waters of these beaches, the risk of infection by enterococci was higher than that posed by E. coli. In Babolsar, the average annual infection risk caused by E. coli and enterococci was 0.365 and 1 for children and 0.181 and 0.986 for adults. Also, in Sari, the average annual infection risk caused by E. coli and enterococci was 0.060 and 0.908 for children and 0.027 and 0.815 for adults. In addition, children were more likely than adults to become infected. Conclusion: In light of the study's findings, due to the entry of untreated urban wastewater into the southern part of the Caspian Sea (northern Iran) and the high risk of infectious diseases for children, more control and health measures are necessary for children's swimming.

Water quality evaluation and non-cariogenic risk assessment of exposure to nitrate in groundwater resources of Kamyaran, Iran: spatial distribution, Monte-Carlo simulation, and sensitivity analysis.

Water is exceptionally vital for all living beings and socio-economic development. This study aimed to investigate the groundwater suitability for drinking in rural areas of Kamyaran city, Kurdistan province, Iran, by using the water quality index (WQI) and evaluating the non-carcinogenic health risk caused by nitrate from the drinking route. Forty-five groundwater samples were collected (2019) from operated dug-wells, and twelve parameters (TDS, pH, TH, EC, HCO3 -, K+, Na+, Mg2+, Ca2+, Cl-, SO4 2-, and NO3 -) were measured to the calculation of WQI. Hazard Quotient (HQ) and sensitivity analysis (SA) using the Monte-Carlo Simulation technique with 10,000 iterations were employed to determine the non-carcinogenic effects of Nitrate in different exposed groups (Infant, children, teenagers, and adults). The results of WQI showed that 74% of groundwater samples fall within the excellent water quality class, and 26% of rural areas fall in the category of good water type. The nitrate concentration in drinking water ranged from 22.42 ± 11.44 mg/L. The HQ mean for infants, children, teenagers, and adults were 0.5606, 0.7288, 0.5606, and 0.438, respectively. Probability estimation showed the HQ values for the 5th, and 95th percentile in infants, children, teenagers, and adult groups were (0.25-1.81), (0.13-1.08), (0.13-0.97), and (0.07-0.51), respectively. The SA showed that the most significant parameter of non-carcinogenic risk in all exposed populations was nitrate concentration. Generally, nitrate concentration in the study area was relatively high, and remarkably in agriculture and fertilizer management required more attention.