[Dietary exposure assessment to aflatoxins in nine kinds of foodstuff by Mont Carlo non-parametric probability approach in Shenzhen City].

OBJECTIVE: To assess Shenzhen residents' dietary exposure to aflatoxins from 9 kinds of foods and health risk. METHODS: Dietary intake of aflatoxins from local concentration data and foodstuff consumption data was simulated by Mont Carlo nonparametric probability approach. RESULTS: Aflatoxins( AFT) average intake and high intake were 0. 8533 ng/( kg·BW·d) and 2. 1646 ng/( kg·BW·d), respectively. Aflatoxin B1( AFB1) average level intake and higher level intake were 0. 5113 ng/( kg·BW·d) and1. 3264 ng/( kg·BW·d), respectively. The potential risk for cancer using average data was estimated at 0. 0199 people/( ten thousand people·year), even though this was0. 0517 people/( ten thousand people·year) in higher level. Major exposure contribution to food were corn and products, rice and products, and peanut oil, corn oil. CONCLUSION: The current dietary intake of AFB1 and AFT in Shenzhen has no appreciable effect on health. Government action should be maintained and improved in order to guarantee, aiming at major exposure contribution to food, that special human exposure levels are kept was low as possible.

[Dietary exposure assessment of aflatoxin of foodstuff and edible oil from Shenzhen residents].

OBJECTIVE: To assess the dietary exposure aflatoxin B1 and total aflatoxins of foodstuff and edible oil in Shenzhen residents. METHODS: Aflatoxins in the samples were determined by the immuno-affinity column clean-up plus UPLC. The aflatoxin B1 and aflatoxins dietary exposure were calculated by the level of aflatoxins contamination in the food and consumption of dietary. RESULTS: The average diary aflatoxin B1 dietary exposure of the man of the 2 to 6, 7 to 14, 15 to 50 and > 50 age group in Shenzhen were 0.320, 0.385, 0.401 and 0.398 ng/(kg BW x d), the results of the woman were 0.282, 0.222, 0.367 and 0.470 ng/(kg BW x d) respectively. The total average daily dietary aflatoxin B1 exposure of the man were 0.012, 0.015, 0.016 and 0.016 ng/(kg BW x d) about each age group. The results of the woman were 78.4, 167, 113 and 103 ng/(kg BW d). According to the the average levels of consumption and the high levels of consumption, the risk of AFB, of the man were 0.012,0.015, 0.016, 0. 016 and 3.0, 8.2, 4.1, 4.4 cancer patient per one hundred thousand, respectively. The results of the woman were 0.010, 0.009, 0.014, 0.018 and 2.9, 6.7, 4.4, 4.0 cancer patient per one hundred thousand, respectively. CONCLUSION: 7 to 14 age group compared with adults age group face higher exposure levels. The rice and peanut oil are most primary aflatoxin dietary exposure sources in Shenzhen.

Dietary exposure to aluminium and health risk assessment in the residents of Shenzhen, China.

Although there are great changes of dietary in the past few decades in China, few are known about the aluminium exposure in Chinese diet. The aim of this study is to systematically evaluate the dietary aluminium intake level in residents of Shenzhen, China. A total of 853 persons from 244 household were investigated their diet by three days food records. Finally, 149 kinds of foods in 17 food groups were selected to be the most consumed foods. From them, 1399 food samples were collected from market to test aluminium concentration. High aluminium levels were found in jellyfish (median, 527.5 mg/kg), fried twisted cruller (median, 466.0 mg/kg), shell (median, 107.1 mg/kg). The Shenzhen residents' average dietary aluminium exposure was estimated at 1.263 mg/kg bw/week which is lower than the PTWI (provisional tolerable weekly intake). But 0-2 and 3-13 age groups have the highest aluminium intake exceeding the PTWI (3.356 mg/kg bw/week and 3.248 mg/kg bw/week) than other age groups. And the main dietary aluminium exposure sources are fried twisted cruller, leaf vegetables and bean products. Our study suggested that even three decades rapid economy development, children in Shenzhen still have high dietary aluminium exposure risk. How to control high dietary aluminium exposure still is a great public health challenge in Shenzhen, China.

[A simple method for assessment of RNA integrity in laser capture microdissection samples].

OBJECTIVE: To develop a simple method for assessment of RNA integrity in laser capture microdissection (LCM) samples. METHODS: The total RNA were isolated from the LCM samples and the sections before and after microdissection and examined by agarose gel electrophoresis. Real-time PCR was employed to assess the RNA from LCM samples, and the quantity of RNA was theoretically estimated according to the average total RNA product in mammalian cells (10 ng/1000 cells). RESULTS: When the total RNA from the sections before and after microdissection was intact, the RNA from LCM samples also had good quality, and the 28S and 18S rRNAs were visualized by ethidium bromide staining. Real-time PCR also showed good RNA quality in the LCM samples. CONCLUSION: A simple method for quantitative and qualitative assessment of the RNA from LCM samples is established, which can also be applied to assessment of DNA or proteins in LCM samples.