1.
Diagn Microbiol Infect Dis
; 87(3): 272-274, 2017 Mar.
Artigo
em Inglês
| MEDLINE
| ID: mdl-27916543
RESUMO
We have developed recombinant fragment C based rapid point of care dipstick devices to assess tetanus immunization status using plasma or whole blood. The devices demonstrated specificity of 0.90 and sensitivity of 0.90 (whole blood)/0.94 (plasma) at field sites in Bangladesh and The Gambia when compared to a commercial ELISA with the immune cut-off titer set as ≥0.1IU/mL.
Assuntos
Anticorpos Antibacterianos/sangue , Fragmentos de Peptídeos/imunologia , Proteínas Recombinantes/imunologia , Toxina Tetânica/imunologia , Toxoide Tetânico/imunologia , Tétano/imunologia , Adulto , Bangladesh , Criança , Gâmbia , Humanos , Masculino , Fragmentos de Peptídeos/genética , Proteínas Recombinantes/genética , Sensibilidade e Especificidade , Toxina Tetânica/genética , Vacinação
2.
Diagn Microbiol Infect Dis
; 82(1): 11-3, 2015 May.
Artigo
em Inglês
| MEDLINE
| ID: mdl-25749462
RESUMO
Anti-tetanus antibodies in biological samples are typically detected using an enzyme-linked immunosorbent assay based on toxoided tetanus neurotoxin as antigen. We demonstrate that recombinantly produced fragment C of the toxin heavy chain is an effective alternative antigen for assessment of tetanus-immune status in plasma samples.