RESUMO
Passiflora is a highly diverse genus where taxonomic lack of consensus remains. This may be the reason why numerous studies do not specify to the infraspecific level the plant material used or lack consistency in the nomenclature of botanical formae of Passiflora edulis. Ultimately, this may contribute to inaccurate chemical composition and health effects attributed to different Passiflora edulis species and formae. Hence, this review aims to overcome these challenges by exploring the phytochemical profile, specific nutritional value and potential health benefits of purple passion fruit (PPF). PPF is often consumed fresh for its pulp (including seeds) or juice, either directly or added to food dishes. It is also used industrially to produce a wide range of products, where peels and seeds are abundant by-products, most often discarded or used in low-value applications. Herein, in a perspective of integral valorisation of the fruit, the potential use of all PPF fractions (peel, pulp and seeds) is discussed as a source of important macro and micronutrients, adequate to integrate a balanced and healthy diet. In addition, the phytochemical profile of such fractions is also discussed along with the associated in vitro biological activities (antioxidant, anti-inflammatory, antibacterial and antifungal) and in vivo beneficial effects in the management of several diseases (asthma, hypertension, osteoarthritis, diabetes and pulmonary fibrosis). In summary, this review gathers the current knowledge on the nutritional and phytochemical composition of PPF and highlights the potential of using all fractions as a source of ingredients in food formulations that promote health and well-being. At the same time, it also contributes to defining sustainable strategies for an integrated valorisation of this natural product.
Assuntos
Passiflora , Frutas/química , Promoção da Saúde , Valor Nutritivo , Passiflora/química , Compostos Fitoquímicos/análiseRESUMO
In this study, the antiradical and antiproliferative effects of the sesquiterpenic compounds trans, trans-farnesol, cis-nerolidol, α-humulene and guaiazulene, commonly found in plants and plant-derived foods and beverages, were evaluated. Chemical (DPPH and hydroxyl radicals) and biological (Caco-2 cells) models were used. Guaiazulene (IC50=0.73mM) showed higher scavenger capacity against DPPH, while trans, trans-farnesol (IC50=1.81mM) and cis-nerolidol (IC50=1.48mM) were more active towards hydroxyl radicals. All compounds, with the exception of α-humulene, were able to protect Caco-2 cells from oxidative stress induced by tert-butyl hydroperoxide. As antiproliferative agents, guaiazulene and cis-nerolidol were more effective than trans, trans-farnesol and α-humulene. The results obtained for the sesquiterpenic compounds by these in vitro assays opens a perspective for their promising use as antioxidants and antiproliferative agents. However, in vivo tests should be carried out in the future to confirm their safety and effectiveness.
Assuntos
Antioxidantes/química , Antioxidantes/farmacologia , Sesquiterpenos/química , Sesquiterpenos/farmacologia , Células CACO-2 , Humanos , Técnicas In Vitro , Extratos Vegetais/químicaRESUMO
Two novel methodologies for quantification of C(13) norisoprenoids in wines were developed. The first methodology, method A (reference method) was based on the headspace solid-phase microextraction combined with gas chromatography-quadrupole mass spectrometry operating in selected ion monitoring mode (HS-SPME-GC-qMS-SIM). This methodology allowed to select the GC conditions for an adequate chromatographic resolution of wine components. The second methodology, method B (rapid method) was based on the HS-SPME-GC-qMS-SIM, using GC conditions that allowed to obtain a C(13) norisoprenoid volatile signature. In the later, the GC capillary column of 30 m at 220 degrees C was used acting as a transfer line of the components sorbed by the SPME coating fibre to the mass spectrometer, which acts as a sensor for m/z fragments 142 and 192. It does not require any pre-treatment of the sample, and the C(13) norisoprenoid composition of the wine was evaluated based on the chromatographic profile and specific m/z fragments, without complete chromatographic separation of its components. For quantification purposes, external calibration curves were constructed with beta-ionone chemical standard. Calibration curves with regression coefficient (r(2)) of 0.9940 and 0.9968, RSD of 1.08% and 12.51%, and detection limits of 1.10 and 1.57 microg L(-1) were obtained for methods A and B, respectively. These methodologies were applied to seventeen white and red table wines. Two vitispirane isomers (158-1529 microg L(-1)) and 1,1,6-trimethyl-1,2-dihydronaphthalene (TDN) (6.42-39.45 microg L(-1)) were quantified. The data obtained for vitispirane isomers and TDN using the two methods were highly correlated (r(2) of 0.9756 and 0.9630, respectively). Associated to the fast and robust character of the proposed rapid method B and considering the extraction time, it is important to focus its selectivity and potential applicability if specific m/z fragments would be established for new analytes.
Assuntos
Cromatografia Gasosa-Espectrometria de Massas/métodos , Norisoprenoides/análise , Vinho/análise , Calibragem , Isótopos de Carbono , Modelos Lineares , Norisoprenoides/química , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Microextração em Fase Sólida , Fatores de TempoRESUMO
Stir bar sorptive extraction with liquid desorption followed by large volume injection coupled to gas chromatography-quadrupole mass spectrometry (SBSE-LD/LVI-GC-qMS) was applied for the quantification of varietal and fermentative volatiles in sparkling wines. The analytical data were performed by using suitable standards of monoterpene hydrocarbons (alpha-pinene), monoterpenols (linalool), sesquiterpenoids (E,E-farnesol, Z-nerolidol, and guaiazulene), C(13) norisoprenoids (beta-ionone), aliphatic and aromatic alcohols (hexanol and 2-phenylethanol), and esters (hexyl acetate and ethyl decanoate) as model compounds. The wine volatiles were quantified using the structurally related standards. The methodology showed good linearity over the concentration range tested, with correlation coefficients ranging from 0.950 to 0.997, and a reproducibility of 9-18%. The SBSE-LD/LVI-GC-qMS methodology allowed, in a single run, the quantification of 71 wine volatiles that can be quantified accurately at levels lower than their respective olfactory thresholds. This methodology was used for assessment of sparkling wine volatiles from different soils, ripening stages, and varieties. The variety and soil influenced significantly the volatile composition of sparkling wines; lower effect was observed for the ripening stage of grapes picked up one week before or after the maturity state.