Nutritional Evaluation and Risk Assessment of the Exposure to Essential and Toxic Elements in Dogs and Cats through the Consumption of Pelleted Dry Food: How Important Is the Quality of the Feed?

Dry feed for pets lacks specific legislation regarding maximum residue limits for inorganic elements. The aim of the present study was to determine the content of 43 inorganic elements in dog and cat feed, studying whether there were differences according to the supposed quality of the food and performing the risk assessment for health. Thirty-one and thirty packages of pelleted dry food for cats and dogs, respectively, were analyzed. After acidic microwave-assisted digestion, elements were detected and quantified by Inductively Coupled Plasma-Mass Spectrometry (ICP-MS). In general, we did not observe important differences in the content of elements according to the supposed quality of the brand. Among trace elements, selenium and manganese are above the dietary reference value. Arsenic and mercury showed the highest acute hazard indexes, which make them risk factors for the health of dogs and cats. Aluminum, uranium, antimony and vanadium contents were above the toxic reference value and showed the highest acute hazard indexes. It is necessary to improve the legislation regarding the food safety of pets, for their health and to protect the rights of consumers.

Risk assessment of the exposure to mycotoxins in dogs and cats through the consumption of commercial dry food.

Dry feed for dogs and cats sold in Europe are mostly formulated with cereals and cereal by-products, so the contamination of this food with mycotoxins represents a potential risk for these pets. We analyzed a representation of the best-selling feed brands in Spain. The presence of Aflatoxins (B1, B2, G1, and G2), Ochratoxin A, T-2 and HT-2 toxins, Deoxynivalenol, Zearalenone, and Fumonisins (B1 and B2) was quantified, using immunoaffinity columns and LC-MS/MS. In general, mycotoxins were frequently and simultaneously (6-11) detected, with AFB1, FB1, FB2, Deoxynivalenol, and HT-2 detected in 100% of the samples. However, the concentrations of most of them are among the lowest reported so far. Fumonisins were the exception since we report the highest concentrations to date, particularly in cat feed. We practically found no significant differences in the level of mycotoxin contamination in relation to the presumed quality of the feed. We also calculated the daily exposure, and evaluated the acute and chronic health risk posed by these feeds. None of the brands analyzed presented acute risk for any of the mycotoxins. However, the high levels of fumonisins found in some samples could become problematic, if there are hidden forms of them. This is also evident in relation to long-term risk, since in the case of fumonisins the level of exposure exceeds the tolerable daily intake level in 3.5 and 12 times, for dogs and cats respectively. The exposure levels to zearalenone and deoxynivalenol could also be of long-term concern, especially considering the possibility that the continuous exposure to several mycotoxins simultaneously might produce potentiated toxic effects as a result of their synergistic action. Further research on the potential adverse health effects deriving from chronic exposure to low doses of multi-mycotoxin mixtures in pets is needed.

Dietary Intake of Essential, Toxic, and Potentially Toxic Elements from Mussels (Mytilus spp.) in the Spanish Population: A Nutritional Assessment.

The levels of forty-three elements were determined in fresh, preserved, and frozen mussels (n = 208) with the purpose of evaluating their contribution to the recommended dietary intake of essential elements and their potential risk to Spanish consumers' health. We found relevant differences in the element content in relation to the mode of conservation of mussels as well as in relation to their geographical origin, brand, or mode of production. According to our estimates, mussels are important contributors to the intake of most essential elements, contributing almost 70% of daily requirements of Se, 30-35% of Mo, Zn, and Co, and around 15% of Fe. At the same time, the pattern of average consumption of mussels in Spain does not seem to imply an excessive risk associated with any of the 36 toxic elements studied. However, it should be noted that, in the high percentile of consumption the exposure to Cd and As may be high, in particular that associated with the consumption of fresh and/or frozen mussels. According to the results of this study, a moderate consumption of mussels can be recommended as a valuable and safe source of trace elements.

Assessment of human health hazards associated with the dietary exposure to organic and inorganic contaminants through the consumption of fishery products in Spain.

In this work we have evaluated the potential carcinogenic and acutely toxic risks associated to the exposure to highly prevalent organic and inorganic contaminants through the consumption of fishery products by the Spanish population. The concentrations of 8 organochlorine pesticides (OCPs), 18 polychlorinated biphenils (PCBs), 7 polycyclic aromatic hydrocarbons (expressed as benzo[a]pyrene toxic equivalents (B[a]Peq)), and three inorganic toxic elements [arsenic (As), cadmium (Cd), and mercury (Hg)] were determined in 93 samples of the most consumed species of white fish, blue fish, cephalopods and seafood species, which were acquired directly in markets and supermarkets in the Canary Islands, Spain. The chemical concentration data were combined with the pattern of consumption of these foodstuffs in order to calculate the daily intake of these contaminants, and on this basis the risk quotients for carcinogenicity and acute toxicity were determined for Spanish adults and children. Our results showed that the daily intake of OCPs, PCBs and B[a]Peq, which is associated to blue fish consumption was the highest within the fish group. The estimated intake of pollutants can be considered low or very low for the individual contaminants, when compared to reference values, except in the case of HCB and As. All the estimated intakes were below the reported Tolerable Daily Intakes. Considering the additive effects of multiple contaminants, the risk of acute toxic effects can also be considered as low or very low. However, our results reflect that the current consumption of white fish in adults and children, and also the blue fish in the case of adults, poses a moderate carcinogenic risk to Spanish consumers, mainly related to their concentrations of As. The conclusions of this research may be useful for the design of appropriate risk communication campaigns.