Low-cost mitigation strategies for community resilience in the Trans-Himalaya to address food and water scarcity in changing environment.

Food and water scarcity in high altitudes of the Trans-Himalayan landscape are being seen as impacts of climate change, which is characterized by prolonged winters with sub-zero temperatures for a longer period. Average daily temperature from Oct to Mar (2020-21) ranged from - 6.73 ± 1.55 to 6.85 ± 1.93 °C; however, fluctuates between - 10.5 °C (minimum) and 4.6 °C (maximum) on a typical day. With minimal rainfall, agriculture is limited to one season in these cold deserts thus limiting the land productivity. The changing scenario affects water availability for agriculture and otherwise in the villages located at further high altitudes dependent on snowmelt flow in nearby streams. This study explores the potential of extended cultivation during winters using low-cost inputs by comparing different technological options for temperature retention for vegetable cultivation. It further demonstrates the role of peoples' participation in developing zero-energy artificial ice reservoirs (water harvesting technology through capturing and storing water in the form of ice) in winters to make water available in the following growing season. With site-specific modifications, an artificial ice reservoir was created by the villagers in 2020-21 which provided additional water for irrigation and was timely available (14 days in advance). Using this additional water, a barren area was brought under the plantation to meet futuristic wood and fodder requirements. Confidence building (knowledge gathered, interventions to solve the major problem of water scarcity) through locally adaptable solutions (portable polyhouse, ice reservoir, increased plant productivity) motivated high altitude villagers in Trans-Himalayan to combat threats of climate change.

Genetic and morphological assessment of a vulnerable large catfish, Silonia silondia (Hamilton, 1822), in natural populations from India.

Silonia silondia is a commercially important fish distributed in Asian countries, which is under threat due to overexploitation. This study focuses on the morphological analysis and genetic variation of S. silondia individuals, through truss network and sequencing of two mitochondrial regions, respectively, from six wild populations of the Ganga and Mahanadi river systems in India. A total of 38 haplotypes was observed by analysing combined mitochondrial genes (cytochrome b + ATPase 6/8) in 247 individuals of S. silondia collected from six populations. Average haplotype and nucleotide diversities were 0.8508 and 0.00231, respectively. Genetic structure analysis showed the predominant cause of genetic variation to be within populations. The two clades were observed among the haplotypes and time of divergence from their most probable ancestor was estimated to be around 0.3949 mya. Analysis of combined mitochondrial genes in six populations of S. silondia resulted into three management units or genetic stocks. The truss network analysis was carried out by interconnecting 12 landmarks from digital images of specimens to identify phenotypic stocks. Sixty-five truss morphometric variables were analysed for geometric shape variation which revealed morphological divergence in River Son specimens. The present study presents molecular markers and genetic diversity data which can be critical input for conservation and management of differentiated populations and future monitoring of the genetic bottleneck. The morphological shape analysis clearly shows that variation in the insertion of adipose fin is an important parameter influencing the morphological discrimination.

Microsatellite marker set for genetic diversity assessment of primitive Chitala chitala (Hamilton, 1822) derived through SMRT sequencing technology.

In present study, single molecule-real time sequencing technology was used to obtain a validated set of microsatellite markers for application in population genetics of the primitive fish, Chitala chitala. Assembly of circular consensus sequencing reads resulted into 1164 sequences which contained 2005 repetitive motifs. A total of 100 sequences were used for primer designing and amplification yielded a set of 28 validated polymorphic markers. These loci were used to genotype n = 72 samples from three distant riverine populations of India, namely Son, Satluj and Brahmaputra, for determining intraspecific genetic variation. The microsatellite loci exhibited high level of polymorphism with PIC values ranging from 0.281 to 0.901. The genetic parameters revealed that mean heterozygosity ranged from 0.6802 to 0.6826 and the populations were found to be genetically diverse (Fst 0.03-0.06). This indicated the potential application of these microsatellite marker set that can used for stock characterization of C. chitala, in the wild. These newly developed loci were assayed for cross transferability in another notopterid fish, Notopterus notopterus.