Nannochloropsis Extract-Mediated Synthesis of Biogenic Silver Nanoparticles, Characterization and In Vitro Assessment of Antimicrobial, Antioxidant and Cytotoxic Activities.

Objective: To investigate the biogenic synthesis of silver nanoparticles (AgNPs) using partially purified ethyl acetate extract of Nannochloropsis sp. hexane (EAENH) fraction of microalga. Methods: The green synthesis of AgNPs was confirmed with UV-Vis spectrum which shows the surface plasmon resonance (SPR) at 421 nm. Fourier Transform Infrared Spectra (FTIR) presented the involvement of functional groups like carboxyl groups of fatty acids, tetraterpenoids of xanthophylls, hydroxyl groups of polyphenols, carbonyl and amide linkage of proteins in the AgNP synthesis. Gas Chromatography-Mass Spectrometry analysis (GCMS) revealed that phytochemicals like octadecanoic acid and hexadecanoic acid imply in capping, bioreduction, and stabilization of AgNps. Result: High-resolution Transmission electron microscope (HRTEM), Dynamic light scattering (DLS), X-ray diffraction (XRD) and EDX analysis showed the crystalline form of the AgNPs with Z-average size 57.25 nm. The zeta potential value of -25.7 mV demonstrated the negative surface charge and colloidal stability of AgNPs. The antimicrobial activity of AgNPs displayed effective inhibition zone against selected bacterial and fungal pathogens. In vitro, antioxidant effects were assessed by 1,1-diphenyl-2-picryl-hydrazyl (DPPH), hydrogen peroxide and reducing power assays which revealed excellent scavenging potential for AgNPs than the extracts. The anti-proliferative potential of biofabricated AgNPs and extracts on Human Non-small lung cancer cell line (A549) was assessed using 3­(4,5-dimethylthiazol-2-yl)-2,5- diphenyl-tetrazolium bromide (MTT) assay with IC50 values of 15 µgmL-1 and 175 µgmL-1 respectively. Conclusion: The study reveals that the microalgae-mediated AgNPs possesses potent antimicrobial and antioxidant activity along with the ability to stimulate apoptosis in A-549 cell line.

Assessment of environmental parameters with special emphasis on avifaunal breeding season in the coastal wetland of Point Calimere Wildlife Sanctuary, Southeast coast of India.

The present study focuses on the hydrographic parameters and the population level of microbial indicators in wetland ecosystems and their effects on bird breeding habitat in Point Calimere Wildlife Sanctuary. Water samples were collected during the bird breeding seasons at five different stations in the sanctuaries, and samples were analyzed by standard methods. Results were compared with CPCB and USEPA standards and clearly denoted that the water quality is not suitable for bird feeding and breeding habitat. One-way ANOVA showed a strong evidence (p < 0.01) of risk for birds breeding in this habitat. As a result of salt pan chemical industries, aquaculture continues to have a major effect on the homogenization and breeding habitat of avian species. Urgent action is needed to prohibit the unregulated economical activities and to regulate water quality monitoring to strictly follow the wildlife conservation rules and regulations. This effective action will help in maintaining species diversity and composition of historical monuments to provide suitable breeding sites in the sanctuary.