Stability and fitness cost associated with spirotetramat resistance in Oxycarenus hyalinipennis Costa (Hemiptera: Lygaeidae).

BACKGROUND: Dusky cotton bug, Oxycarenus hyalinipennis Costa (Hemiptera: Lygaeidae) is an important pest of cotton and causing economic losses to this crop. It also remains active round the year, infesting a number of host plants. Spirotetramat is a systemic insecticide and is effective against many sucking insect pests. A field collected population of O. hyalinipennis was reared in the laboratory under continuous spirotetramat selection pressure for 21 generations for the development of resistance to spirotetramat. The Spiro-Sel population was further reared for seven generations without insecticide exposure to assess the stability of spirotetramat resistance. Leaf dip method was used for the bioassays and selection. In this study, the impact of spirotetramat resistance on its stability and life history traits of Spiro-Sel, C1 (15 Spiro-Sel♀ × 15 UNSEL ♂) and C2 (15 Spiro-Sel♂ × 15 UNSEL ♀) O. hyalinipennis was assessed. RESULTS: Spiro-Sel (G21 ) population developed 2333-fold and 20.83-fold resistance compared with the susceptible and unselected (UNSEL) populations, respectively. Resistance to spirotetramat was unstable after seven generations (G28 ) when reared without exposure to any insecticide. A significant reduction in overall nymphal survival, fecundity, egg hatching and net reproductive rate of Spiro-Sel population was observed when compared with UNSEL population. Intrinsic rate of natural increase, biotic potential and mean relative growth rate were also lower in Spiro-Sel population compared to UNSEL population. The Spiro-Sel, C1 and C2 population had a relative fitness of 0.44, 0.51 and 0.44, respectively. CONCLUSION: Results of our study suggested that fitness cost is involved in the development of spirotetramat resistance. Unstable resistance and high fitness cost may provide great benefits to limit the evolution of resistance to spirotetramat in O. hyalinipennis. © 2021 Society of Chemical Industry.

Sulfoxaflor resistance in Oxycarenus hyalinipennis (Costa) induces negligible cross-resistance to other tested insecticides: stability, risk assessment, and fitness cost.

BACKGROUND: Oxycarenus hyalinipennis (Costa) (Hemiptera: Lygaeidae) is a polyphagous insect pest. In Pakistan, O. hyalinipennis is managed by using different insecticides, a major concern for cotton producers, and has developed resistance to many of these. Sulfoxaflor belongs to a newly released sulfoximine family that has high efficacy for controlling sap-feeding insect pests. RESULTS: A sulfoxaflor selected-population (Sulfo-Sel-Pop) of O. hyalinipennis showed a 3064.92-fold level of resistance after continuous selection for 18 generations with sulfoxaflor compared to unselected population (UNSel-Pop). The Sulfo-Sel-Pop showed a reduction in resistance (from 3064.92 to 635-fold) without exposure for five generations, indicating unstable resistance to sulfoxaflor. Cross-resistance studies of the Sulfo-Sel-Pop revealed no or very low cross-resistance to triazophos (0.42 to 0.30-fold), deltamethrin (0.85 to 0.18-fold) and acetamiprid (1.16 to 4.86-fold) from G4 to G19 compared to the field population (Field-Pop). The mean value of realized heritability was 0.15 in the Sulfo-Sel-Pop (G19 ). Significantly reduced relative fitness was determined in the Sulfo-Sel-Pop (Rf = 0.21) followed by Cross1 [(Sulfo-Sel-Pop ♂ × UNSel-Pop ♀) (Rf = 0.58)], and Cross2 [(Sulfo-Sel-Pop ♀ × UNSel-Pop ♂) (Rf = 0.70)] compared with the UNSel-Pop. The values of intrinsic rate of natural increase (rm ), biotic potential (Bp) fecundity, egg hatching, and net reproductive rate (R0) were also significantly less in the Sulfo-Sel-Pop compared to UNSel-Pop. CONCLUSION: A very high fitness cost, unstable resistance and no or very low cross-resistance in the Sulfo-Sel-Pop have great implications in designing effective strategies for managing insecticide resistance to O. hyalinipennis. © 2021 Society of Chemical Industry.

Monitoring and assessment of resistance to insecticides in the lacewing Chrysoperla carnea (Stephens).

Chrysoperla carnea (Stephens) is a key predator of several soft-bodied arthropods across various cropping ecosystems. Biological control is a prime strategy to reduce the harmful effects of pesticides on environment especially plants and arthropods. Collection of lacewings (Field Populations) was made during 2014 and repeated in 2016 from five different districts composed mainly by row and vegetable crops under conventional cropping system. For these populations, resistance level to four insecticides representing different mode of action was measured using topical treatment of 1st-instar larvae with prepared dilutions of commercial formulation. The highest resistance levels were found for chlorfenapyr (100.86- to 423.78-fold) and indoxacarb (131.28- to 255.05-fold) in populations from all districts, while buprofezin and nitenpyram resistance was low to high and moderate to high, respectively. The levels of resistance to nitenpyram increased from 2014 to 2016 for populations of 3 out of 4 surveyed areas, while the susceptibility to buprofezin remained unchanged. Decrease in resistance levels for chlorfenapyr and indoxacarb in 2 years was marginal. These results suggest that field populations of C. carnea maintained resistance to buprofezin, chlorfenapyr, indoxacarb, and nitenpyram. Current outcomes support the fact that this bio-control agent could be a main nominee for mass release under judicious application of selective insecticides.