Uncovering Potentially Therapeutic Phytochemicals, In silico Analysis, and Biological Assessment of South-Chinese Red Dragon Fruit (Hylocereus polyrhizus).

Red dragon fruit is gaining popularity globally due to its nutritional value and bioactive components. The study aimed to assess the phytochemical, nutritional composition, antioxidant, antibacterial, and cytotoxic properties of extracts from the South Chinese red dragon fruit peel, flesh, and seeds. Extract fractions with increasing polarity (ethyl acetate

Comparative analysis of gut fungal composition and structure of the yaks under different feeding models.

The yaks that inhabit the Tibetan plateau are a rare breed that is closely related to local economic development and human civilization. This ancient breed may have evolved a unique gut microbiota due to the hypoxic high-altitude environment. The gut microbiota is susceptible to external factors, but research regarding the effects of different feeding models on the gut fungal community in yaks remains scarce. In this study, we compared and analyzed the composition and variability of the gut fungal community among wild yaks (WYG), house-feeding domestic yaks (HFG), and grazing domestic yaks (GYG). The results revealed that Basidiomycota and Ascomycota were the most preponderant phyla in the gut fungal community, regardless of feeding models. Although the types of dominant fungal phyla did not change, their abundances did. Intergroup analysis of fungal diversity showed that the Shannon and Simpson indices of WYG and GYG were significantly higher than those of HFG. Fungal taxonomic analysis showed that there were 20 genera (Sclerostagonospora and Didymella) that were significantly different between WYG and GYG, and 16 genera (Thelebolus and Cystobasidium) that were significantly different between the WYG and HFG. Furthermore, the proportions of 14 genera (Claussenomyces and Papiliotrema) significantly decreased, whereas the proportions of eight genera (Stropharia and Lichtheimia) significantly increased in HFG as compared to GYG. Taken together, this study indicated that the gut fungal composition and structure differ significantly between yaks raised in different breeding groups.

Assessment of lithium bioaccumulation by quinoa (Chenopodium quinoa willd.) and its implication for human health.

Lithium (Li) is the lightest alkali metal and 27th most abundant element in the earth crust. In traces, the element has medicinal value for various disorders in humans, however, its higher concentrations may lead to treatment-resistant depression and altered thyroid functioning. Quinoa (Chenopodium quinoa) has gained popularity owing to its halophytic nature and its potential use as an alternative to the traditional staple foods. However, quinoa response to Li-salt in terms of growth, Li accumulation potential and health risks associated with consumption of the quinoa seeds grown on Li-contaminated soils has not been explored yet. During this study, quinoa was exposed to various concentrations of Li (0, 2, 4, 8 and 16 mM) at germination as well as seedling stages. The results showed that seed germination was the highest (64% higher than control) at Li concentration of 8 mM. Similarly, at 8 mM doses of Li shoot length, shoot dry weight, root length, root dry weight and grain yield were increased by 130%, 300%, 244%, 858% and 185% than control. It was also revealed that Li increased the accumulation of calcium and sodium in quinoa shoots. Carotenoids contents were increased, but chlorophyll contents remained un-changed under Li application. The activities of antioxidants viz. Peroxide dismutase, catalase and super oxide dismutase were also increased with an increase in the levels of Li in the soil. Estimated daily intake and hazard quotient of Li in quinoa were less than the threshold level. It was concluded that Li concentration of 8 mM is useful for quinoa growth and it can be successfully grown on Li contaminated soils without causing any human health risks.

Diphtheria in Pakistan post-COVID-19, a potential public health threat: an update.

Diphtheria, a vaccine-preventable disease, remains a concern in Pakistan as cases have risen post-COVID-19 pandemic causing more than 45 deaths in Pakistan in the year 2022. The respiratory variant of the disease is more common and can lead to serious complications, such as myocarditis and respiratory insufficiency. Diphtheria has caused havoc in the past killing millions of people worldwide before the development of its vaccine. Although the diphtheria toxoid vaccine is effective against toxigenic strains, there have been cases of treatment-resistant strains, particularly the non-toxigenic strains of C. diphtheriae. Pakistan's economic and health systems have suffered setbacks, which have been exacerbated by the COVID-19 pandemic. The pandemic has disrupted routine vaccination programs, and recent floods have contributed to an increase in diphtheria cases and rendered millions homeless. Poor immunization services, inadequate training of vaccination teams, and wealth inequality have all contributed to unequal vaccination coverage in Pakistan. The rising cases of diphtheria call for prompt action, including booster shots, updating vaccination records and administering immediate doses of the toxoid to close contacts.

Municipal solid waste treatment for bioenergy and resource production: Potential technologies, techno-economic-environmental aspects and implications of membrane-based recovery.

World estimated municipal solid waste generating at an alarming rate and its disposal is a severe concern of today's world. It is equivalent to 0.79 kg/d per person footprint and causing climate change; health hazards and other environmental issues which need attention on an urgent basis. Waste to energy (WTE) considers as an alternative renewable energy potential to recover energy from waste and reduce the global waste problems. WTE reduced the burden on fossil fuels for energy generation, waste volumes, environmental, and greenhouse gases emissions. This critical review aims to evaluate the source of solid waste generation and the possible routes of waste management such as biological landfill and thermal treatment (Incineration, pyrolysis, and gasification). Moreover, a comparative evaluation of different technologies was reviewed in terms of economic and environmental aspects along with their limitations and advantages. Critical literature revealed that gasification seemed to be the efficient route and environmentally sustainable. In addition, a framework for the gasification process, gasifier types, and selection of gasifiers for MSW was presented. The country-wise solutions recommendation was proposed for solid waste management with the least impact on the environment. Furthermore, key issues and potential perspectives that require urgent attention to facilitate global penetration are highlighted. Finally, practical implications of membrane and comparison membrane-based separation technology with other conventional technologies to recover bioenergy and resources were discussed. It is expected that this study will lead towards practical solution for future advancement in terms of economic and environmental concerns, and also provide economic feasibility and practical implications for global penetration.

Risk assessment of trace element accumulation in soil and Brassica oleracea after wastewater irrigation.

The risk assessment of trace elements has received substantial attention for the achievement of UN Sustainable Developmental Goals (UN-SDGs). The present study aimed to evaluate health and ecological risks associated with trace element accumulation in Brassica oleracea under wastewater irrigations from three different areas. This study, for the first time, compared the pros and cons of mixed water crop irrigation (wastewater with fresh/groundwater). A pot experiment was conducted to evaluate the buildup of eight trace elements (As, Cu, Cd, Mn, Fe, Pb, Ni and Zn) in soil and B. oleracea plants irrigated with wastewater alone and mixed with fresh/groundwater. Specific ecological [degree of contamination (Cd), potential ecological risk index (PERI), pollution load index (PLI), geo-accumulation index (Igeo)], phytoaccumulation [bioconcentration factor (BCF) and transfer factor (TF)] and health risk models [chronic daily intake (CDI), hazard quotient (HQ), cancer risk (CR)] were applied to assess the overall contamination of trace elements in the soil-plant-human system. Moreover, these indices were compared with the literature data. The concentration of Cd, Fe and Mn exceeded the threshold limits of 10, 500 and 200 mg kg-1, respectively, for agricultural soil. Overall, all the irrigation waters caused significant pollution load in soil indicating high ecological risk (Cd > 24, PERI > 380, Igeo > 5, PLI > 2). Not all the mixing treatments caused a reduction in trace element buildup in soil. The mixing of wastewater-1 with either groundwater or freshwater increased trace element levels in the soil as well as risk indices compared to wastewater alone. The BCF and TF values were > 1, respectively, for 66% and 7% treatments. Trace element concentration in plants and associated health risk were minimized in mixed wastewater treatments. There were 22% and 32% reduction in HQ and CR when wastewater was mixed with freshwater and 29% and 8% when mixed with groundwater. Despite total reduction, a great variation in % change in risk indices was observed with respect to the area of wastewater collection. Therefore, mixed water irrigation may be a good management strategy, but its recommendation depends on soil properties and composition of waters used for mixing. Moreover, it is recommended that the freshwater and wastewater of the particular area may be continuously monitored to avoid potential associated health hazards.

Spatial distribution, health risk assessment, and public perception of groundwater in Bahawalnagar, Punjab, Pakistan: a multivariate analysis.

The current study investigated the hydrogeochemical behavior of groundwater quality attributes including arsenic (As) and their associated health risks in unexplored groundwater aquifers of Bahawalnagar, Punjab, Pakistan. The groundwater samples were collected from 40 colonies of Bahawalnagar city from electric/hand pumps, tube wells and turbines installed at varying depth (20 to > 100 m). The groundwater possessed the highest concentrations of PO4 (0.5 mg/L), HCO3 (425 mg/L), Cl (623 mg/L), NO3 (136.68 mg/L) and SO4 (749.7 mg/L) concentrations. There was no difference in concentration of As in shallow and deep aquifers. Interestingly, none of the water samples showed As concentration higher than the WHO limit of 10 µg/L for drinking water with groundwater As concentration spanning from 2.5 to 7.9 µg/L. The HQ values for As were less than 1 and there was no apparent non-carcinogenic risk from the long-term consumption of As contaminated water. The questionnaire survey indicated that 82% respondents believe that drinking water quality affects human health and 55% of respondents considered that groundwater in the area is not suitable for drinking. Survey results revealed that 29.11, 22.78, 17.08, 15.19, 7.59, 5.06 and 3.16% respondents/family members suffered from hepatitis, skin problems, diabetes, tuberculosis, kidney disorders, muscular weakness and gastro, respectively. However, the data cannot be correlated with As contamination and disease burden in the local community and it can be anticipated that the groundwater may contain other potentially toxic ions that are deteriorating the water quality and compromising human health. The hydrogeochemical analysis revealed Na-Cl/SO4, K-SO4 type of groundwater suggesting the potential role of sulfate containing minerals in releasing As in the groundwater aquifers.

Situational assessment for fecal sludge management in major cities of Pakistan.

With enhanced focus on global sanitation, access to toilets at the household level is increasing in developing countries although the provision of sewer networks is not expanding at the same pace. This is resulting in the adaptation of on-site sanitation facilities to contain the fecal sludge. The fecal sludge generated by the on-site sanitation facilities requires emptying, treatment, and safe end-use or disposal. In this study, the sanitation situation and need for fecal sludge management was evaluated in major cities of Pakistan including Karachi (provincial capital), Lahore (provincial capital), and Islamabad (national capital). Primary and secondary data were collected from key informant interviews of the stakeholders, national and international reports, research, and review articles. Infographics on wastewater and fecal sludge from origin to disposal were developed using a shit flow diagram tool and enabling environment was evaluated with a modified service delivery assessment tool. The results indicate that sewerage network coverage exists for 60%, 63%, and 50% of the areas in Karachi, Lahore, and Islamabad respectively. The sewerage network in major cities is old, leaking, and insufficient, thus a limited amount of wastewater reaches the treatment plants. Total wastewater treatment in Karachi and Islamabad is 10% and 9% respectively whereas, in Lahore, there is no infrastructure for the same. The safe sanitation in Lahore (8%) and Islamabad (25%) is coming from on-site sanitation systems with fecal sludge buried safely onsite. National level sanitation programs exist in the country but are limited to reducing open defecation and containments of fecal sludge only. The inclusion of complete fecal sludge management related framework, guidelines, and policies can help achieve the goal of safe sanitation for all.

Biowaste-based sorbents for arsenic removal from aqueous medium and risk assessment.

Water contamination by arsenic (As) is widespread and is posing serious health threats globally. Hence, As removal techniques/adsorbents need to be explored to minimize potentials hazards of drinking As-contaminated waters. A column scale sorption experiment was performed to assess the potential of three biosorbents (tea waste, wheat straw and peanut shells) to remove As (50, 100, 200 and 400 µg L-1) from aqueous medium at a pH range of 5-8. The efficiency of agricultural biosorbents to remove As varies greatly regarding their type, initial As concentration in water and solution pH. It was observed that all of the biosorbents efficiently removed As from water samples. The maximum As removal (up to 92%) was observed for 400 µg L-1 initial As concentration. Noticeably, at high initial As concentrations (200 and 400 µg L-1), low pH (5 and 6) facilitates As removal. Among the three biosorbents, tea waste biosorbent showed substantial ability to minimize health risks by removing As (up to 92%) compared to peanut shells (89%) and wheat straw (88%). Likewise, the values of evaluated risk parameters (carcinogenic and non-carcinogenic risk) were significantly decreased (7-92%: average 66%) after biosorption experiment. The scanning electron microscopy, Fourier transform infrared spectroscopy, energy-dispersive X-ray and X-ray diffraction analyses confirmed the potential of biosorbents to remediate As via successful loading of As on their surfaces. Hence, it can be concluded that synthesized biosorbents exhibit efficient and ecofriendly potential for As removal from contaminated water to minimize human health risk.

A Brief Assessment on Recent Developments in Efficient Electrocatalytic Nitrogen Reduction with 2D Non-Metallic Nanomaterials.

In recent years, the synthesis of ammonia (NH3) has been developed by electrocatalytic technology that is a potential way to effectively replace the Haber-Bosch process, which is an industrial synthesis of NH3. Industrial ammonia has caused a series of problems for the population and environment. In the face of sustainable green synthesis methods, the advantages of electrocatalytic nitrogen reduction for synthesis of NH3 in aqueous media have attracted a great amount of attention from researchers. This review summarizes the recent progress on the highly efficient electrocatalysts based on 2D non-metallic nanomaterial and provides a brief overview of the synthesis principle of electrocatalysis and the performance measurement indicators of electrocatalysts. Moreover, the current development of N2 reduction reaction (NRR) electrocatalyst is discussed and prospected.

Accumulation pattern and risk assessment of potentially toxic elements in selected wastewater-irrigated soils and plants in Vehari, Pakistan.

There are scarce data about the accumulation pattern and risk assessment of potentially toxic elements (PTEs) in soil and associated potential ecological risks, especially in less-developed countries. This study aims to assess the pollution levels and potential ecological risks of PTEs (As, Cr, Cd, Cu, Ni, Mn, Pb and Zn) in wastewater-irrigated arable soils and different edible-grown plants in selected areas of Vehari, Pakistan. The results revealed that the values of PTEs in soil samples were higher than their respective limit values by 20% for As, 87% for Cd, 15% for Cu, 2% for Cr, 83% for Mn, 98% for Fe, and 7% for Zn. The values of soil risk indices such as the potential ecological risk (PERI >380 for all samples), pollution load index (PLI >4 for 94% of studied samples), and degree of contamination (Dc > 24 for all samples) showed severe soil contamination in the study area. Some vegetables exhibited a high metal accumulation index (e.g., 8.1 for onion), signifying potential associated health hazards. Thus, long-term wastewater irrigation has led to severe soil contamination, which can pose potential ecological risks via PTE accumulation in crops, particularly Cd. Therefore, to ensure food safety, frequent wastewater irrigation practices need to be minimized and managed in the study area.

Comparison of Different Nutritional Screening Approaches and the Determinants of Malnutrition in Under-Five Children in a Marginalized District of Punjab Province, Pakistan.

OBJECTIVES: This research measures the occurrence of malnutrition amongst under-five children in the Rahimyar Khan district of Southern Punjab in Pakistan. Employing different anthropometric measurement approaches such as (1) conventional indices (HAZ, WAZ, and WHZ), (2) CIAF, (3) BMI-for-age, and (4) MUAC, we compare their estimated results and examine the relationship between socioeconomic determinants and different anthropometric indicators. METHODS: The study employs a proportional purposive random sampling method to collect data from 384 rural households in the community-based study using a self-administered survey and following the Lady Health Workers (LHWs) registered records. The nutritional status of 517 under-five children is measured with references to WHO (2009) child growth standards. Furthermore, the investigation used the model of binary logistic regression to measure the impact of socioeconomic factors on child malnutrition. RESULTS: Compared with other approaches, the CIAF identifies more malnourished children (63%). The results of binary logistic regression illustrate that all the explanatory variables indicate a more significant empirical association with CIAF than conventional indices, BMI-for-age, and MUAC. CONCLUSION: CIAF is a more reliable tool for assessing child nutrition because it not only demonstrates more accurate estimates of malnutrition but also recognizes children with multiple anthropometric failures.

Comparative in-vitro anti-inflammatory, anticholinesterase and antidiabetic evaluation: computational and kinetic assessment of succinimides cyano-acetate derivatives.

This research was planned to synthesize cyano-acetate derivatives of succinimide and evaluate its comparative biological efficacy as anti-inflammatory, anti-cholinesterase and anti-diabetic, which was further validated by molecular docking studies. The three cyano-acetate derivatives of succinimide including compound 23 Methyl 2-cyano-2-(2,5-dioxopyrrolidin-3-yl)acetate, compound 31 Methyl 2-cyano-2-(1-methyl-2,5-dioxopyrrolidin-3-yl)acetate and compound 44 Methyl 2-cyano-2-(1-ethyl-2,5-dioxopyrrolidin-3-yl) acetate were synthesized. The mentioned compounds were checked for in vitro anti-inflammatory, anti-cholinesterase and anti-diabetic (α-amylase inhibition) activity. To validate the in vitro results, computational studies were carried out using molecular operating environment to analyse the BE, i.e. binding energies of all synthesized compounds against the respective enzymes. The Compounds 23, 31, 44 exhibited anti-inflammatory via inhibiting COX-2 (IC50 value of 204.08, 68.60 and 50.93 µM, respectively), COX-1 (IC50 value of 287, 185, and 143 µM, respectively) and 5-LOX (IC50 value of 138, 50.76 and 20, 87 µM respectively). They exhibited choline-mimetic potential, such as compound 23, 31 and 44 inhibited AChE enzyme (IC50 value of 240, 174, and 134 µM, respectively) and BChE enzyme (IC50 value of 203, 134 and 97 µM, respectively). The Compounds 23, 31, 44 exhibited anti-diabetic effect via inhibiting α-amylase enzyme (IC50 values of 250, 106 and 60 µM, respectively). Molecular docking studies revealed that the synthesized compounds have good binding affinity in the binding pockets of AChE, BChE, COX-2, 5-LOX and α-amylase enzyme and showed high binding energies. The synthesized succinimide derivatives, i.e. compound 23, 31, 44 showed marked inhibitory activities against cyclooxygenase, lipoxygenase, α-amylase and cholinesterase enzymes. Among these three, compound 44 and 31 showed strong anti-inflammatory and anti-diabetic activity while they displayed moderate anti-cholinesterase activity supported by molecular docking results.Communicated by Ramaswamy H. Sarma.

Distribution and ecological risk assessment of trace elements in the paddy soil-rice ecosystem of Punjab, Pakistan.

Trace elements (TEs) contamination of agricultural soils requires suitable criteria for regulating their toxicity limits in soil and food crops, which depends on their potential ecological risk spanning regional to global scales. However, no comprehensive study is available that links TE concentrations in paddy soil with ecological and human health risks in less developed regions like Pakistan. Here we evaluated the data set to establish standard guidelines for defining the hazard levels of various potentially toxic TEs (such as As, Cd, Co, Cu, Cr, Fe, Mn, Ni, Pb, Se, Zn) in agricultural paddy soils of Punjab, Pakistan. In total, 100 topsoils (at 0-15 cm depth) and 204 rice plant (shoot and grain) samples were collected from five ecological zones of Punjab (Gujranwala, Hafizabad, Vehari, Mailsi, and Burewala), representing the major rice growing regions in Pakistan. The degree of contamination (Cd) and potential ecological risk index (PERI) established from ecological risk models were substantially higher in 100% and 97% of samples, respectively. The positive matrix factorization (PMF) model revealed that the elevated TEs concentration, notably Cd, As, Cr, Ni, and Pb, in the agricultural paddy soil was attributed to the anthropogenic activities and groundwater irrigation. Moreover, the concentration of these TEs in rice grains was higher than the FAO/WHO's safe limits. This study provided a baseline, albeit critical knowledge, on the impact of TE-allied ecological and human health risks in the paddy soil-rice system in Pakistan; and it opens new avenues for setting TEs guidelines in agro-ecological zones globally, especially in underdeveloped regions.

Socio-Economic and Environmental Determinants of Malnutrition in under Three Children: Evidence from PDHS-2018.

OBJECTIVES: This research investigates the association of malnutrition with social and economic factors in general and environmental factors in specific such as sanitation facilities and drinking water sources for Pakistan. METHODS: Authors used the latest data of 1010 Under-Three children from Pakistan Demographic and Health Survey (PDHS) 2017-2018. Cumulative Index of Anthropometric Failure (CIAF) was developed to measure the malnutrition status among children based on z-scores of WHZ, WAZ, and HAZ, respectively. The study has applied the discrete-choice logistic methodology to find the relationship of malnutrition with socio-economic characteristics. The interaction terms of drinking water source and sanitation facility have been measured to see the impact of environmental factors on child malnutrition. RESULTS: The study results depict that the likelihood of malnutrition increases when the child had diarrhea recently and the child belongs to the deprived region such as KPK, Sind, and Baluchistan. However, the chances of child malnutrition drop with (1) an escalation of mothers' education, (2) a rise in wealth status of the household, and (3) the improved water source and sanitation facility in the household. The only water-improved sanitation category of the interaction term is significant in the model which depicts that households having both improved water and improved sanitation facilities had very fewer chances of malnutrition among their children. CONCLUSION: Authors conclude that malnutrition in younger children is associated with improved water as well as sanitation facilities, maternal education, and household wealth in Pakistan.

Morphological and palynological assessment of taxonomically problematic genus Paspalum based on light and scanning electron microscopy.

Present research work was carried out to clarify the variations among species of genus Paspalum morphologically and palynologically as this genus is taxonomically difficult due to having multiple similar morphologically overlapping characters which make it difficult to identify. Henceforth present research work was carried out to delimit taxa within the same genus by morphological and palynological tools through light microscopy (LM) and scanning electron microscopy (SEM). Both these tools are considered as the most useful taxonomic characters for taxonomically problematic genera. The results showed a lot of variations among morphological characters. In Paspalum dilatatum, the upper glume was ovate whereas in the other two species, the upper glume was elliptic. The upper glume apex found in P. dilatatum and Paspalum scrobiculatum was obtuse whereas in Paspalum distichum, upper glume apex was acute. Glume nerves showed variation in all three species. Paspalum distichum was 3 nerved, P. scrobiculatum was 5-7 nerved, and P. dilatatum was 5-9 nerved. All three species showed variation in lemma nerves. Paspalum scrobiculatum had 3 nerved lemma whereas in P. distichum 3-5 nerved and P. dilatatum 5-9 nerved lemma were present. In polar and equatorial view, pollen grains ranged from 25 (20-30) to 37.5 (30-45) µm. Paspalum distichum appeared to be the smallest in size whereas P. dilatatum was the largest. Exine thickness ranged from 0.75 (0.5-1) to 1.35 (1.2-1.5) µm. The higher value of pollen fertility was found in P. scrobiculatum as 87.69% and the lowest value was in P. distichum as 78.08%. Morphological keys were also given for correct identification.

Rural mothers' beliefs and practices about diagnosis, treatment, and management of children health problems: A qualitative study in marginalized Southern Pakistan.

Introduction: Appropriate health-seeking beliefs and practices are indispensable for the survival and development of children. In this study, we explore childcare beliefs and practices of rural mothers and analyze the different ways childhood illness is diagnosed and managed in a marginalized rural community in Southern Pakistan. Methods: Using purposive sampling, in-depth interviews are conducted to obtain qualitative data from 20 illiterate and rural mothers in addition to 15 healthcare providers in the district Rajanpur of South Punjab. Results and discussion: The findings reveal that rural mothers' access to healthcare and therapeutic programs is impeded due to geographical isolation, structural inequalities, poverty, and illiteracy. Consequently, evil eyes, witchcraft, and spirits are recognized as potential threats to children's health and nutrition. Therefore, the treatment of childhood morbidity and malnutrition is mostly performed with folk, domestic, herbal, magico-religious remedies, and spiritual healing methods. The current study also highlights that many low-income and rural mothers tend to normalize childhood illness when they become unable to advocate for their children's health and nutrition. Besides improving low-income mothers' access to healthcare facilities, health education and risk communication at the field level through field health staff could be most effective for health promotion.

The Effects of Financial Stress and Household Socio-Economic Deprivation on the Malnutrition Statuses of Children under Five during the COVID-19 Lockdown in a Marginalized Region of South Punjab, Pakistan.

The lockdown after the COVID-19 pandemic not only caused public health crises and income stress but also put millions at risk of food insecurity and malnutrition across the globe, especially in low and middle-income countries [LMICs]. This study evaluated the effects of financial stress and household socio-economic deprivation on the nutritional status of 1551 children under the age of five during COVID-19 in Pakistan. A self-administered questionnaire was used between November 2020 and April 2021 to collect information on age, height, children's weight, and socio-economic status from 1152 rural households from underdeveloped regions in Punjab, Pakistan. With the help of the proportionate simple random sampling method, this study employed a model (binary logistic regression) to calculate the likelihood of malnourishment. The findings showed that the stunting, underweight, and wasting prevalence rates during COVID-19 were 58.86%, 41.89%, and 8.11%, respectively, in the Bahawalpur region. According to the binary logistic regression analysis, among the household deprivation status (HDS) categories, the risks of childhood malnutrition were lesser in HDS-2 (OR = 0.05, 95% CI: 0. 005-0.879) and HDS-3 (OR = 0.04, 95% CI: 0.008-0.193). Similar to this, within the financial stress index (FSI) categories, the children in homes with medium financial stress had reduced odds of malnutrition (OR = 0.10, 95% CI: 0.018-0.567), and the children in households with low financial stress had reduced risks of malnutrition (OR = 0.006, 95% CI: 0.005-0.061). The proposed research found that stunting and underweight increased by 17.26% and 12.29% during the COVID-19 lockdown in the Bahawalpur region. Additionally, financial stress and socio-economic deprivation strongly affected children's nutritional statuses during the COVID-19 lockdown in the Bahawalpur region of Southern Punjab.

A Qualitative Exploration in Causes of Water Insecurity Experiences, and Gender and Nutritional Consequences in South-Punjab, Pakistan.

Although water insecurity has been discussed in general, its impacts on mothers' physical and mental health, and infants' and young children's feeding (IYCF), has largely been ignored. This study explores household water insecurity experiences and their association with optimal health and nutrition of women and children in the Rajanpur district of Punjab Province. Using focus group discussions (FGDs) and key informants (KIIs) interviews from an area with high maternal-child malnutrition prevalence, a qualitative study was conducted to describe local experiences of water acquirement and arrangement, and of the consequences of water insecurity. The findings highlight that rural Western marginalized populations of the Rajanpur district rely on brackish, canal, or flood surface water as the water supply is absent, which intensifies mothers' work burden and stress, and often makes them victims of violence, stigma, and sickness. Water fetching impacts women in unforeseen ways, impacting the psychosocial and physical health of mothers engaged in maternal breastfeeding. Water insecurity, originally rooted in regional disparities, compounds with gender inequities, which leads to maternal stress and child sickness. Justice in water resources is imperative and urgent in the deprived South of Punjab province for improving public health nutrition.

Family Care, Economic Stress, and Depressive Symptoms Among Chinese Adults During the COVID-19 Outbreak: Difference by Urban and Rural Areas.

Background: Although several studies have shown an association of family care with a high level of depressive symptoms, the relationship between them remains indistinguishable. Objective: This study aims to examine the associations between family care, economic stress, and depressive symptoms among Chinese adults in urban and rural areas during the COVID-19 outbreak. Methods: Based on cross-sectional data collected through online surveys from February 1st to 10th 2020 in China the present study recruited 2,858 adults. It used multiple linear regression to examine the association between family care and depressive symptoms, while economic stress was examined as moderators on the above relationship. Results: The results showed that caring for both the elderly and children was significantly associated with higher depressive symptoms compared with non-caregivers (B = 2.584, 95%CI: 1.254, 3.915), and a similar result was also found in urban areas. Also, caring for the elderly only was also had a higher level of depressive symptoms than non-caregivers in rural areas (B = 3.135, 95%CI: 0.745, 5.525). Meeting the care needs was significantly associated with lower depressive symptoms compared with unmet care needs, while for rural caregivers, the results were not significant. Besides, economic stress strengthened the effect of family care needs on depressive symptoms for sandwich-generation caregivers who provide care to both the elderly and children (B = 0.605, 95%CI: 0.077, 1.134). While in rural areas, the moderation effects of economic stress were only found for elderly caregivers (B = 1.106, 95%CI: 0.178, 2.035). Conclusion: These findings suggest that we should pay more attention to the family caregiver's mental health during the COVID-19 outbreak. In addition, more effective policies should be developed to provide financial support for family caregivers, especially for sandwich-generation caregivers and rural elderly caregivers.