Utility of Proteomics in Emerging and Re-Emerging Infectious Diseases Caused by RNA Viruses.

Emerging and re-emerging infectious diseases due to RNA viruses cause major negative consequences for the quality of life, public health, and overall economic development. Most of the RNA viruses causing illnesses in humans are of zoonotic origin. Zoonotic viruses can directly be transferred from animals to humans through adaptation, followed by human-to-human transmission, such as in human immunodeficiency virus (HIV), severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome coronavirus (MERS-CoV), and, more recently, SARS coronavirus 2 (SARS-CoV-2), or they can be transferred through insects or vectors, as in the case of Crimean-Congo hemorrhagic fever virus (CCHFV), Zika virus (ZIKV), and dengue virus (DENV). At the present, there are no vaccines or antiviral compounds against most of these viruses. Because proteins possess a vast array of functions in all known biological systems, proteomics-based strategies can provide important insights into the investigation of disease pathogenesis and the identification of promising antiviral drug targets during an epidemic or pandemic. Mass spectrometry technology has provided the capacity required for the precise identification and the sensitive and high-throughput analysis of proteins on a large scale and has contributed greatly to unravelling key protein-protein interactions, discovering signaling networks, and understanding disease mechanisms. In this Review, we present an account of quantitative proteomics and its application in some prominent recent examples of emerging and re-emerging RNA virus diseases like HIV-1, CCHFV, ZIKV, and DENV, with more detail with respect to coronaviruses (MERS-CoV and SARS-CoV) as well as the recent SARS-CoV-2 pandemic.

Self Assessment of Hearing Quality and Noise-Related Attitudes among Traffic Policemen of Patiala, India.

The study was conducted on a group of 90 traffic policemen aged 20-50 years and working in and around Patiala district to assess their knowledge, attitude, and practices toward the ill effects caused by traffic noise on health. The subjects were required to fill up a questionnaire regarding their self-assessment of hearing ability, noise-related attitudes, and the use of personal protective equipment such as earplugs. The mean age of subjects was 39.8 ± 5.80 years, while the mean duration of exposure to traffic noise was 10.56 ± 6.43 years. Only 5.5% subjects felt that their hearing was below average. As many as 61% subjects had work-related tinnitus. It was observed that none of them had ever used ear plugs, mainly due to their non availability. The self-assessment of hearing by traffic policemen indicated that most of them had normal hearing. However, to find out the exact number of cases with hearing impairment, further evaluation with audiometry is recommended in them.

Peak expiratory flow rate of healthy school children living at high altitude.

BACKGROUND: Anthropometric and socioeconomic factors are known to influence peak expiratory flow rate, but the effect of altitude has not been established decisively, due to conflicting results of various studies. AIM: This study was conducted to evaluate the effect of altitude on peak expiratory flow rate of healthy school children. MATERIALS AND METHODS: A cross-sectional study was conducted in which the peak expiratory flow rate of a group of 290 school children (140 male and 150 female), aged 7-14 years, residing in Shimla (altitude 2150 meters), were compared with the results obtained in an age- and sex-matched control group, consisting of 280 school children (138 male and 142 female), residing in Patiala (altitude 278 meters). Mini Wright Peak Flow Meter was used for the study. RESULTS: The mean peak expiratory flow rate value of boys at high altitude (265 ± 92.6 L/min) was significantly higher than those in plain areas (245 ± 82.1 L/min). Similarly, the mean peak expiratory flow rate of highlander girls (250.4 ± 70.2 L/min) was significantly higher than girls of plains (232.6 ± 65 L/min). CONCLUSION: The study suggests that besides anthropometric and socioeconomic factors, altitude is an important determinant of lung function.