Detection of the mcr-1 gene in colistin-resistant Escherichia coli from retail meat in Japan.

In this study, the presence of the mcr-1 gene in Escherichia coli from retail meat in Japan was investigated. Nine E. coli isolates (eight from chickens and one from pork) carried the mcr-1 gene on the plasmid. In six isolates from domestic chickens, mcr-1 was located on the IncI2 plasmid, which is approximately 60 kb in size. In the remaining three isolates from imported chicken and pork, mcr-1 was located on the IncX4 plasmid (30 kb).

In vitro assessment of mandibular single/two implant-retained overdentures using stress-breaking attachments.

OBJECTIVE: This study investigated the strain surround implants and the pressure distribution of the alveolar ridge related to implant overdentures with stress-breaking ball (SBB) attachments. METHODS: Simulation models of edentulous mandibles were embedded with 1 and 2 implants to simulate a single implant-retained overdenture (S-IOD) and a two-implant-retained overdenture (T-IOD), respectively. The implants were connected to the denture base with the following conditions: complete separation between the denture base and implant with cover screws as a conventional complete denture (CD), 3 types of settlements with an SBB attachment between them, and a conventional ball attachment (BALL). Strain surround the implant, pressure at 5 different soft tissue areas, and displacement of the denture base were measured with loading up to 50 N. RESULTS: In S-IOD and T-IOD, the strain of each SBB attachment was less than the strain shown by the BALL. The pressure at each region of the SBB attachment was less than the pressure shown with the CD and BALL. CONCLUSIONS: SBB attachments prevented strain surround the implants in loading and provided optimal stress distribution.

Effect of implant support on distal-extension removable partial dentures: in vivo assessment.

PURPOSE: The use of a limited number of implants for support of a removable partial denture (RPD) changes a Kennedy Class I or II situation to that of a Class III. This in vivo pilot study evaluated implant-supported distal-extension removable partial dentures (RPD) in 5 partially edentulous patients. MATERIALS AND METHODS: Two implants (Brånemark TU MK III, Nobel Biocare) were placed in a mandibular Kennedy Class I arch. To fabricate an implant-supported RPD (ISRPD), a conventional RPD base was fitted to the healing abutment with autopolymerizing acrylic resin (Uni-fast II, GC) to support the posterior aspect of the RPD. By changing the healing abutment to a healing cap, there was no connection between the denture base and implant, and the ISRPD became a conventional RPD (CRPD). Using a crossover study design, the masticatory movements (mandibular movements during mastication) of both dentures were measured using a commercially available tracking device (BioPACK, Bioresearch, Japan). The occlusal force and contact area were also measured using pressure-sensitive sheets and an image scanner (T-scan system). Using a visual analog scale (VAS), the 4 criteria of comfort, chewing, retention, and stability were evaluated. All the data obtained were analyzed using Wilcoxen signed rank tests (alpha = .05). RESULTS: There were no significant differences (P > .05) in masticatory movements between the ISRPD and the CRPD (5 patients: 4 women, 1 man). However, the ISRPD had significantly greater force and greater area than the CRPD (P = .043). The center of occlusal force of the ISRPD tended to move more distally compared to the CRPD. All the patients preferred the ISRPD for comfort, chewing, retention, and stability. CONCLUSIONS: One implant per edentulous area and a simple attachment technique yielded a stable distal extension RPD.