RESUMO
In this work, determination of aspartic acid by N-doped carbon dots (N-CDs) was studied at optimum condition. Characterization and morphology of surface of N-CDs were carried out by FT-IR and HRTEM. N-doped carbon dots size was 10 nm. Quenching was very fast after addition of aspartic acid that is an important property of this sensor. Optimum conditions for pH and excitation wavelength were 8 and 360 nm, respectively. Linear dynamic range and limit of detection for aspartic acid were 0.5-50 µM and 90 nM, respectively. This method was used for aspartic acid determination in human serum and sport supplement powder as real samples. Performance of this sensor was also compared with other fluorescent sensors.
Assuntos
Ácido Aspártico/análise , Carbono/química , Técnicas de Química Analítica/instrumentação , Custos e Análise de Custo , Micro-Ondas , Nitrogênio/química , Esportes , Ácido Aspártico/sangue , Técnicas de Química Analítica/economia , Técnicas de Química Sintética , Humanos , Limite de Detecção , Pontos Quânticos/química , Espectrometria de Fluorescência , Fatores de TempoRESUMO
The antioxidant and antibacterial activities of six edible plants were evaluated. The active constituents of the edible plants were extracted using boiling water or 80% methanol. Results demonstrated that extraction of antioxidants by boiling water was more efficient. Lycium depressum and Berula angustifolia had the highest antioxidant activities and, therefore, could be rich sources of natural antioxidants. The antibacterial activities of the extracts were tested against Staphylococcus aureus, Escherichia coli, Entrococcus faecalis and Proteus mirabilis. Further research is needed to isolate, characterize, and identify the bioactive compounds present in these plants.
