RESUMO
By means of bioluminiscence ATP present in a sample can be quantified. Thus, it would be a method able to evaluate microbiological or organic matter (from vegetal or animal origin) contamination. The present work analyzes the possibility to assess--from the microbiological point of view--the air of pharmaceutical clean rooms by means of bioluminiscence, using the luminomiter HY-LITE 2 [Merck]. It is thought that the use of this methodology versus microbiological classical methods, will allow to obtain results in the working day. Classical methods demand a minimum of 72 hs incubation to read results. But the real conclusion is that with the used technology, it is not possible to evaluate microbiologically the air of pharmaceutical clean rooms.